Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1960 and GFF1961 are separated by 267 nucleotides GFF1961 and GFF1962 overlap by 11 nucleotides
GFF1960 - Protease VII (Omptin) precursor (EC 3.4.23.49), at 152,445 to 153,383
GFF1960
GFF1961 - Phosphoglycerate transport system transcriptional regulatory protein PgtA, at 153,651 to 154,898
GFF1961
GFF1962 - Phosphoglycerate transport system sensor protein PgtB (EC 2.7.3.-), at 154,888 to 156,894
GFF1962
Position (kb)
153
154
155 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 152.698 kb on + strand, within GFF1960 at 152.742 kb on - strand, within GFF1960 at 152.820 kb on - strand, within GFF1960 at 152.989 kb on + strand, within GFF1960 at 153.001 kb on + strand, within GFF1960 at 153.154 kb on - strand, within GFF1960 at 154.088 kb on - strand, within GFF1961 at 154.302 kb on - strand, within GFF1961 at 154.315 kb on - strand, within GFF1961 at 154.315 kb on - strand, within GFF1961 at 154.370 kb on + strand, within GFF1961 at 154.371 kb on - strand, within GFF1961 at 154.371 kb on - strand, within GFF1961 at 154.650 kb on - strand, within GFF1961 at 154.724 kb on + strand, within GFF1961 at 154.794 kb on - strand at 154.849 kb on - strand at 155.057 kb on - strand at 155.106 kb on - strand, within GFF1962 at 155.229 kb on + strand, within GFF1962 at 155.336 kb on + strand, within GFF1962 at 155.485 kb on + strand, within GFF1962 at 155.555 kb on - strand, within GFF1962 at 155.755 kb on + strand, within GFF1962 at 155.756 kb on - strand, within GFF1962 at 155.756 kb on - strand, within GFF1962 at 155.756 kb on - strand, within GFF1962 at 155.761 kb on + strand, within GFF1962 at 155.868 kb on + strand, within GFF1962
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 152,698 + GFF1960 0.27 -0.2 152,742 - GFF1960 0.32 -0.4 152,820 - GFF1960 0.40 -1.1 152,989 + GFF1960 0.58 +0.7 153,001 + GFF1960 0.59 +0.5 153,154 - GFF1960 0.76 -2.9 154,088 - GFF1961 0.35 +0.6 154,302 - GFF1961 0.52 -1.2 154,315 - GFF1961 0.53 -0.7 154,315 - GFF1961 0.53 +0.0 154,370 + GFF1961 0.58 -0.4 154,371 - GFF1961 0.58 -1.6 154,371 - GFF1961 0.58 -2.5 154,650 - GFF1961 0.80 -1.3 154,724 + GFF1961 0.86 -0.2 154,794 - +0.4 154,849 - +0.2 155,057 - +0.4 155,106 - GFF1962 0.11 +0.3 155,229 + GFF1962 0.17 +0.1 155,336 + GFF1962 0.22 +0.1 155,485 + GFF1962 0.30 +1.1 155,555 - GFF1962 0.33 -0.1 155,755 + GFF1962 0.43 -2.0 155,756 - GFF1962 0.43 -1.5 155,756 - GFF1962 0.43 +0.4 155,756 - GFF1962 0.43 +1.0 155,761 + GFF1962 0.43 +0.3 155,868 + GFF1962 0.49 +0.9
Or see this region's nucleotide sequence