Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1847 and GFF1848 are separated by 425 nucleotides GFF1848 and GFF1849 are separated by 109 nucleotides GFF1849 and GFF1850 are separated by 5 nucleotides
GFF1847 - Anaerobic glycerol-3-phosphate dehydrogenase subunit C (EC 1.1.5.3), at 39,503 to 40,693
GFF1847
GFF1848 - FIG074102: hypothetical protein, at 41,119 to 42,141
GFF1848
GFF1849 - Molybdopterin binding motif, CinA N-terminal domain / C-terminal domain of CinA type E, at 42,251 to 42,427
GFF1849
GFF1850 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.-), at 42,433 to 43,236
GFF1850
Position (kb) 41
42
43 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 40.135 kb on + strand, within GFF1847 at 40.204 kb on + strand, within GFF1847 at 40.234 kb on + strand, within GFF1847 at 40.235 kb on - strand, within GFF1847 at 40.301 kb on - strand, within GFF1847 at 40.385 kb on + strand, within GFF1847 at 40.385 kb on + strand, within GFF1847 at 40.385 kb on + strand, within GFF1847 at 40.391 kb on - strand, within GFF1847 at 40.473 kb on + strand, within GFF1847 at 40.639 kb on + strand at 40.695 kb on + strand at 40.763 kb on - strand at 40.772 kb on - strand at 40.772 kb on - strand at 40.812 kb on + strand at 40.848 kb on - strand at 40.906 kb on + strand at 40.916 kb on + strand at 41.174 kb on + strand at 41.562 kb on - strand, within GFF1848 at 42.127 kb on + strand at 42.207 kb on + strand at 42.211 kb on + strand at 42.215 kb on + strand at 42.331 kb on + strand, within GFF1849 at 42.365 kb on + strand, within GFF1849 at 42.377 kb on - strand, within GFF1849 at 42.379 kb on - strand, within GFF1849 at 42.519 kb on + strand, within GFF1850 at 42.520 kb on - strand, within GFF1850 at 42.520 kb on - strand, within GFF1850 at 42.627 kb on - strand, within GFF1850 at 42.680 kb on + strand, within GFF1850 at 42.823 kb on - strand, within GFF1850 at 42.823 kb on - strand, within GFF1850 at 42.824 kb on + strand, within GFF1850 at 42.825 kb on - strand, within GFF1850 at 42.825 kb on - strand, within GFF1850 at 42.999 kb on + strand, within GFF1850 at 43.000 kb on - strand, within GFF1850 at 43.054 kb on + strand, within GFF1850 at 43.065 kb on - strand, within GFF1850 at 43.102 kb on + strand, within GFF1850 at 43.103 kb on - strand, within GFF1850 at 43.105 kb on + strand, within GFF1850
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 40,135 + GFF1847 0.53 +0.1 40,204 + GFF1847 0.59 -1.2 40,234 + GFF1847 0.61 -0.9 40,235 - GFF1847 0.61 +0.2 40,301 - GFF1847 0.67 +0.6 40,385 + GFF1847 0.74 -0.8 40,385 + GFF1847 0.74 -2.8 40,385 + GFF1847 0.74 -0.2 40,391 - GFF1847 0.75 +0.1 40,473 + GFF1847 0.81 -1.9 40,639 + -0.6 40,695 + -0.1 40,763 - +0.0 40,772 - -0.5 40,772 - -0.1 40,812 + -1.2 40,848 - -0.2 40,906 + -1.9 40,916 + -0.5 41,174 + +1.9 41,562 - GFF1848 0.43 +0.2 42,127 + +1.1 42,207 + -0.8 42,211 + +0.2 42,215 + -0.2 42,331 + GFF1849 0.45 +0.4 42,365 + GFF1849 0.64 +0.5 42,377 - GFF1849 0.71 -1.0 42,379 - GFF1849 0.72 -0.8 42,519 + GFF1850 0.11 -1.2 42,520 - GFF1850 0.11 -0.7 42,520 - GFF1850 0.11 +1.3 42,627 - GFF1850 0.24 -0.2 42,680 + GFF1850 0.31 -0.1 42,823 - GFF1850 0.49 +0.5 42,823 - GFF1850 0.49 +0.4 42,824 + GFF1850 0.49 +0.6 42,825 - GFF1850 0.49 -0.3 42,825 - GFF1850 0.49 -0.5 42,999 + GFF1850 0.70 +0.5 43,000 - GFF1850 0.71 -0.8 43,054 + GFF1850 0.77 +0.3 43,065 - GFF1850 0.79 -0.1 43,102 + GFF1850 0.83 -0.9 43,103 - GFF1850 0.83 +0.2 43,105 + GFF1850 0.84 -1.4
Or see this region's nucleotide sequence