Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1829 and GFF1830 are separated by 728 nucleotides GFF1830 and GFF1831 are separated by 16 nucleotides GFF1831 and GFF1832 are separated by 102 nucleotides
GFF1829 - Outer membrane protein C precursor, at 11,836 to 12,972
GFF1829
GFF1830 - Two-component sensor protein RcsD (EC 2.7.3.-), at 13,701 to 16,370
GFF1830
GFF1831 - DNA-binding capsular synthesis response regulator RcsB, at 16,387 to 17,037
GFF1831
GFF1832 - Two-component sensor protein RcsC (EC 2.7.3.-), at 17,140 to 19,986
GFF1832
Position (kb) 13
14
15
16
17 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 12.806 kb on + strand, within GFF1829 at 12.806 kb on + strand, within GFF1829 at 12.807 kb on - strand, within GFF1829 at 12.807 kb on - strand, within GFF1829 at 12.898 kb on - strand at 12.991 kb on + strand at 12.999 kb on + strand at 13.536 kb on + strand at 13.624 kb on - strand at 13.692 kb on + strand at 13.771 kb on + strand at 13.813 kb on + strand at 13.906 kb on - strand at 13.999 kb on - strand, within GFF1830 at 14.081 kb on + strand, within GFF1830 at 14.081 kb on + strand, within GFF1830 at 14.812 kb on - strand, within GFF1830 at 14.889 kb on - strand, within GFF1830 at 14.949 kb on + strand, within GFF1830 at 15.104 kb on + strand, within GFF1830 at 15.203 kb on - strand, within GFF1830 at 15.212 kb on + strand, within GFF1830 at 15.213 kb on - strand, within GFF1830 at 15.297 kb on - strand, within GFF1830 at 15.297 kb on - strand, within GFF1830 at 15.338 kb on + strand, within GFF1830 at 15.359 kb on + strand, within GFF1830 at 15.362 kb on + strand, within GFF1830 at 15.470 kb on + strand, within GFF1830 at 15.580 kb on - strand, within GFF1830 at 15.680 kb on + strand, within GFF1830 at 15.711 kb on - strand, within GFF1830 at 15.747 kb on - strand, within GFF1830 at 15.747 kb on - strand, within GFF1830 at 15.880 kb on + strand, within GFF1830 at 15.892 kb on - strand, within GFF1830 at 16.020 kb on - strand, within GFF1830 at 16.036 kb on + strand, within GFF1830 at 16.036 kb on + strand, within GFF1830 at 16.036 kb on + strand, within GFF1830 at 16.128 kb on + strand at 16.163 kb on + strand at 16.173 kb on + strand at 16.385 kb on - strand at 16.396 kb on - strand at 16.431 kb on - strand at 16.923 kb on + strand, within GFF1831 at 17.046 kb on + strand at 17.047 kb on - strand at 17.075 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 12,806 + GFF1829 0.85 +0.8 12,806 + GFF1829 0.85 -0.4 12,807 - GFF1829 0.85 -2.5 12,807 - GFF1829 0.85 -0.1 12,898 - -0.5 12,991 + +0.5 12,999 + -0.1 13,536 + +0.6 13,624 - +0.0 13,692 + -0.6 13,771 + -2.4 13,813 + -0.3 13,906 - -1.4 13,999 - GFF1830 0.11 -1.3 14,081 + GFF1830 0.14 -0.8 14,081 + GFF1830 0.14 -2.2 14,812 - GFF1830 0.42 +0.3 14,889 - GFF1830 0.44 -2.5 14,949 + GFF1830 0.47 -3.5 15,104 + GFF1830 0.53 -0.3 15,203 - GFF1830 0.56 -2.1 15,212 + GFF1830 0.57 -1.4 15,213 - GFF1830 0.57 -3.0 15,297 - GFF1830 0.60 -0.4 15,297 - GFF1830 0.60 -1.4 15,338 + GFF1830 0.61 -2.6 15,359 + GFF1830 0.62 -3.1 15,362 + GFF1830 0.62 -3.5 15,470 + GFF1830 0.66 -2.5 15,580 - GFF1830 0.70 +0.6 15,680 + GFF1830 0.74 -3.7 15,711 - GFF1830 0.75 -0.4 15,747 - GFF1830 0.77 -2.4 15,747 - GFF1830 0.77 -1.4 15,880 + GFF1830 0.82 -1.4 15,892 - GFF1830 0.82 -0.9 16,020 - GFF1830 0.87 -1.4 16,036 + GFF1830 0.87 -1.4 16,036 + GFF1830 0.87 -0.5 16,036 + GFF1830 0.87 -3.9 16,128 + -1.6 16,163 + -1.9 16,173 + -1.2 16,385 - -1.9 16,396 - -3.9 16,431 - +0.4 16,923 + GFF1831 0.82 -2.7 17,046 + -1.9 17,047 - -0.5 17,075 + -1.9
Or see this region's nucleotide sequence