Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1675 and GFF1676 overlap by 26 nucleotides GFF1676 and GFF1677 are separated by 632 nucleotides
GFF1675 - Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15), at 59,721 to 61,142
GFF1675
GFF1676 - Trehalose-6-phosphate phosphatase (EC 3.1.3.12), at 61,117 to 61,920
GFF1676
GFF1677 - DJ-1/YajL/PfpI superfamily, includes chaperone protein YajL (former ThiJ), parkinsonism-associated protein DJ-1, peptidases PfpI, Hsp31, at 62,553 to 63,110
GFF1677
Position (kb)
61
62 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 60.142 kb on + strand, within GFF1675 at 60.227 kb on - strand, within GFF1675 at 60.475 kb on + strand, within GFF1675 at 60.475 kb on + strand, within GFF1675 at 60.476 kb on - strand, within GFF1675 at 60.829 kb on + strand, within GFF1675 at 60.962 kb on + strand, within GFF1675 at 60.968 kb on + strand, within GFF1675 at 61.118 kb on + strand at 61.193 kb on - strand at 61.576 kb on + strand, within GFF1676 at 61.592 kb on - strand, within GFF1676 at 61.648 kb on - strand, within GFF1676 at 61.787 kb on - strand, within GFF1676 at 61.976 kb on + strand at 61.976 kb on + strand at 61.981 kb on - strand at 62.008 kb on + strand at 62.109 kb on + strand at 62.111 kb on + strand at 62.222 kb on - strand at 62.346 kb on - strand at 62.436 kb on - strand at 62.524 kb on + strand at 62.566 kb on + strand at 62.567 kb on - strand at 62.571 kb on - strand at 62.571 kb on - strand at 62.704 kb on + strand, within GFF1677 at 62.750 kb on - strand, within GFF1677 at 62.904 kb on + strand, within GFF1677
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 60,142 + GFF1675 0.30 +0.1 60,227 - GFF1675 0.36 -2.5 60,475 + GFF1675 0.53 +1.1 60,475 + GFF1675 0.53 -2.1 60,476 - GFF1675 0.53 -0.6 60,829 + GFF1675 0.78 +1.1 60,962 + GFF1675 0.87 +2.5 60,968 + GFF1675 0.88 +0.1 61,118 + -0.1 61,193 - -0.7 61,576 + GFF1676 0.57 -0.6 61,592 - GFF1676 0.59 -0.3 61,648 - GFF1676 0.66 +0.1 61,787 - GFF1676 0.83 -1.9 61,976 + +0.5 61,976 + -1.2 61,981 - +0.0 62,008 + -0.5 62,109 + +0.2 62,111 + -0.1 62,222 - -1.6 62,346 - -0.5 62,436 - -2.5 62,524 + -0.3 62,566 + -0.7 62,567 - -0.4 62,571 - +0.3 62,571 - +0.1 62,704 + GFF1677 0.27 +1.3 62,750 - GFF1677 0.35 -0.3 62,904 + GFF1677 0.63 -1.3
Or see this region's nucleotide sequence