Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF165 and GFF166 are separated by 2 nucleotides GFF166 and GFF167 are separated by 10 nucleotides GFF167 and GFF168 are separated by 20 nucleotides GFF168 and GFF169 are separated by 201 nucleotides
GFF165 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 156,923 to 158,014
GFF165
GFF166 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 158,017 to 159,417
GFF166
GFF167 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 159,428 to 160,033
GFF167
GFF168 - hypothetical protein, at 160,054 to 160,167
GFF168
GFF169 - Sugar-phosphate stress protein SgrT (embedded in SgrS), at 160,369 to 160,491
GFF169
Position (kb) 158
159
160 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 157.028 kb on - strand at 157.033 kb on + strand, within GFF165 at 157.033 kb on + strand, within GFF165 at 157.034 kb on - strand, within GFF165 at 157.034 kb on - strand, within GFF165 at 157.069 kb on + strand, within GFF165 at 157.498 kb on + strand, within GFF165 at 157.515 kb on + strand, within GFF165 at 157.516 kb on - strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.596 kb on - strand, within GFF165 at 157.596 kb on - strand, within GFF165 at 157.596 kb on - strand, within GFF165 at 157.596 kb on - strand, within GFF165 at 157.597 kb on + strand, within GFF165 at 157.598 kb on - strand, within GFF165 at 157.648 kb on - strand, within GFF165 at 157.648 kb on - strand, within GFF165 at 157.854 kb on - strand, within GFF165 at 157.982 kb on - strand at 158.012 kb on + strand at 158.032 kb on + strand at 158.092 kb on + strand at 158.232 kb on + strand, within GFF166 at 158.278 kb on - strand, within GFF166 at 158.278 kb on - strand, within GFF166 at 158.309 kb on - strand, within GFF166 at 158.326 kb on + strand, within GFF166 at 158.472 kb on - strand, within GFF166 at 158.653 kb on - strand, within GFF166 at 158.740 kb on + strand, within GFF166 at 158.744 kb on + strand, within GFF166 at 158.744 kb on + strand, within GFF166 at 158.744 kb on + strand, within GFF166 at 158.745 kb on - strand, within GFF166 at 158.839 kb on - strand, within GFF166 at 158.989 kb on + strand, within GFF166 at 158.989 kb on + strand, within GFF166 at 158.990 kb on - strand, within GFF166 at 159.416 kb on - strand at 159.424 kb on + strand at 159.443 kb on - strand at 159.502 kb on + strand, within GFF167 at 159.508 kb on + strand, within GFF167 at 159.646 kb on - strand, within GFF167 at 159.721 kb on + strand, within GFF167 at 159.722 kb on - strand, within GFF167 at 159.759 kb on - strand, within GFF167 at 160.024 kb on + strand at 160.295 kb on - strand at 160.317 kb on + strand at 160.317 kb on + strand at 160.337 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 157,028 - -0.3 157,033 + GFF165 0.10 +0.2 157,033 + GFF165 0.10 -3.1 157,034 - GFF165 0.10 -0.6 157,034 - GFF165 0.10 +1.0 157,069 + GFF165 0.13 -2.4 157,498 + GFF165 0.53 -0.8 157,515 + GFF165 0.54 -0.3 157,516 - GFF165 0.54 -0.5 157,595 + GFF165 0.62 +1.0 157,595 + GFF165 0.62 +0.4 157,595 + GFF165 0.62 -1.0 157,595 + GFF165 0.62 -1.2 157,595 + GFF165 0.62 -0.2 157,595 + GFF165 0.62 -0.2 157,596 - GFF165 0.62 -0.1 157,596 - GFF165 0.62 -0.6 157,596 - GFF165 0.62 +1.3 157,596 - GFF165 0.62 +1.5 157,597 + GFF165 0.62 -0.2 157,598 - GFF165 0.62 -1.5 157,648 - GFF165 0.66 -0.2 157,648 - GFF165 0.66 +0.7 157,854 - GFF165 0.85 +0.1 157,982 - +0.2 158,012 + -0.2 158,032 + +0.8 158,092 + +0.5 158,232 + GFF166 0.15 -0.6 158,278 - GFF166 0.19 +0.4 158,278 - GFF166 0.19 -0.6 158,309 - GFF166 0.21 -1.2 158,326 + GFF166 0.22 +0.1 158,472 - GFF166 0.32 +0.2 158,653 - GFF166 0.45 -0.2 158,740 + GFF166 0.52 -0.3 158,744 + GFF166 0.52 -1.4 158,744 + GFF166 0.52 +0.7 158,744 + GFF166 0.52 -0.5 158,745 - GFF166 0.52 +1.2 158,839 - GFF166 0.59 -1.3 158,989 + GFF166 0.69 -0.4 158,989 + GFF166 0.69 -0.3 158,990 - GFF166 0.69 +0.4 159,416 - -0.5 159,424 + -1.4 159,443 - +0.1 159,502 + GFF167 0.12 +0.5 159,508 + GFF167 0.13 +0.8 159,646 - GFF167 0.36 +0.2 159,721 + GFF167 0.48 -0.6 159,722 - GFF167 0.49 +0.7 159,759 - GFF167 0.55 +1.4 160,024 + +0.8 160,295 - +0.4 160,317 + -0.2 160,317 + -1.2 160,337 + +0.8
Or see this region's nucleotide sequence