Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1589 and GFF1590 are separated by 164 nucleotides GFF1590 and GFF1591 are separated by 496 nucleotides
GFF1589 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 46,462 to 47,457
GFF1589
GFF1590 - MFS permease protein, at 47,622 to 48,806
GFF1590
GFF1591 - Transcription repressor of tripartite multidrug resistance system, at 49,303 to 49,833
GFF1591
Position (kb) 47
48
49 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 46.630 kb on + strand, within GFF1589 at 46.631 kb on - strand, within GFF1589 at 46.632 kb on + strand, within GFF1589 at 46.637 kb on + strand, within GFF1589 at 46.638 kb on - strand, within GFF1589 at 46.667 kb on + strand, within GFF1589 at 46.672 kb on + strand, within GFF1589 at 46.672 kb on + strand, within GFF1589 at 46.673 kb on - strand, within GFF1589 at 46.737 kb on + strand, within GFF1589 at 46.737 kb on + strand, within GFF1589 at 46.738 kb on - strand, within GFF1589 at 46.738 kb on - strand, within GFF1589 at 46.741 kb on + strand, within GFF1589 at 46.741 kb on + strand, within GFF1589 at 46.742 kb on - strand, within GFF1589 at 47.020 kb on + strand, within GFF1589 at 47.020 kb on + strand, within GFF1589 at 47.020 kb on + strand, within GFF1589 at 47.078 kb on - strand, within GFF1589 at 47.078 kb on - strand, within GFF1589 at 47.078 kb on - strand, within GFF1589 at 47.111 kb on + strand, within GFF1589 at 47.229 kb on + strand, within GFF1589 at 47.230 kb on - strand, within GFF1589 at 47.238 kb on + strand, within GFF1589 at 47.238 kb on + strand, within GFF1589 at 47.239 kb on - strand, within GFF1589 at 47.239 kb on - strand, within GFF1589 at 47.385 kb on - strand at 47.455 kb on + strand at 47.455 kb on + strand at 47.558 kb on + strand at 47.559 kb on - strand at 47.665 kb on + strand at 47.704 kb on - strand at 47.704 kb on - strand at 47.930 kb on - strand, within GFF1590 at 48.208 kb on - strand, within GFF1590 at 48.229 kb on + strand, within GFF1590 at 48.354 kb on + strand, within GFF1590 at 48.444 kb on - strand, within GFF1590 at 48.578 kb on + strand, within GFF1590 at 48.578 kb on + strand, within GFF1590 at 48.578 kb on + strand, within GFF1590 at 48.578 kb on + strand, within GFF1590 at 48.579 kb on - strand, within GFF1590 at 48.600 kb on + strand, within GFF1590 at 48.601 kb on - strand, within GFF1590 at 48.642 kb on + strand, within GFF1590 at 48.645 kb on - strand, within GFF1590 at 48.655 kb on - strand, within GFF1590 at 48.655 kb on - strand, within GFF1590 at 48.834 kb on - strand at 49.401 kb on + strand, within GFF1591 at 49.515 kb on + strand, within GFF1591 at 49.731 kb on + strand, within GFF1591 at 49.770 kb on + strand, within GFF1591
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 46,630 + GFF1589 0.17 -1.8 46,631 - GFF1589 0.17 +0.1 46,632 + GFF1589 0.17 -0.5 46,637 + GFF1589 0.18 -0.7 46,638 - GFF1589 0.18 +0.9 46,667 + GFF1589 0.21 -2.0 46,672 + GFF1589 0.21 -2.3 46,672 + GFF1589 0.21 +0.4 46,673 - GFF1589 0.21 +0.3 46,737 + GFF1589 0.28 -0.2 46,737 + GFF1589 0.28 +0.5 46,738 - GFF1589 0.28 +0.7 46,738 - GFF1589 0.28 -0.5 46,741 + GFF1589 0.28 +0.0 46,741 + GFF1589 0.28 +0.8 46,742 - GFF1589 0.28 -1.0 47,020 + GFF1589 0.56 +0.2 47,020 + GFF1589 0.56 +0.2 47,020 + GFF1589 0.56 -0.3 47,078 - GFF1589 0.62 +1.1 47,078 - GFF1589 0.62 -3.2 47,078 - GFF1589 0.62 +0.3 47,111 + GFF1589 0.65 -0.2 47,229 + GFF1589 0.77 +0.7 47,230 - GFF1589 0.77 +0.2 47,238 + GFF1589 0.78 -0.5 47,238 + GFF1589 0.78 -0.0 47,239 - GFF1589 0.78 +0.3 47,239 - GFF1589 0.78 -0.6 47,385 - +0.6 47,455 + +0.4 47,455 + +1.4 47,558 + +0.4 47,559 - -0.1 47,665 + -0.0 47,704 - +0.2 47,704 - +0.1 47,930 - GFF1590 0.26 -1.0 48,208 - GFF1590 0.49 -0.3 48,229 + GFF1590 0.51 -1.0 48,354 + GFF1590 0.62 -4.6 48,444 - GFF1590 0.69 +0.1 48,578 + GFF1590 0.81 +1.3 48,578 + GFF1590 0.81 +0.1 48,578 + GFF1590 0.81 -0.8 48,578 + GFF1590 0.81 +1.1 48,579 - GFF1590 0.81 -0.4 48,600 + GFF1590 0.83 +0.1 48,601 - GFF1590 0.83 +0.1 48,642 + GFF1590 0.86 -1.2 48,645 - GFF1590 0.86 -0.5 48,655 - GFF1590 0.87 -1.6 48,655 - GFF1590 0.87 -2.1 48,834 - +0.2 49,401 + GFF1591 0.18 -0.7 49,515 + GFF1591 0.40 -0.8 49,731 + GFF1591 0.81 +2.2 49,770 + GFF1591 0.88 +1.8
Or see this region's nucleotide sequence