Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1551 and GFF1552 are separated by 470 nucleotides GFF1552 and GFF1553 are separated by 98 nucleotides GFF1553 and GFF1554 overlap by 4 nucleotides
GFF1551 - Similar to pipB, at 14,984 to 16,084
GFF1551
GFF1552 - virulence protein, at 16,555 to 17,484
GFF1552
GFF1553 - FIG01046564: hypothetical protein, at 17,583 to 17,780
GFF1553
GFF1554 - Putative transcriptional regulator, at 17,777 to 18,673
GFF1554
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 15.720 kb on - strand, within GFF1551 at 15.785 kb on - strand, within GFF1551 at 15.836 kb on - strand, within GFF1551 at 15.929 kb on - strand, within GFF1551 at 15.955 kb on + strand, within GFF1551 at 15.955 kb on + strand, within GFF1551 at 16.170 kb on - strand at 16.604 kb on + strand at 16.715 kb on - strand, within GFF1552 at 16.815 kb on + strand, within GFF1552 at 16.816 kb on - strand, within GFF1552 at 16.955 kb on + strand, within GFF1552 at 16.956 kb on - strand, within GFF1552 at 16.959 kb on + strand, within GFF1552 at 16.960 kb on - strand, within GFF1552 at 16.963 kb on + strand, within GFF1552 at 16.964 kb on - strand, within GFF1552 at 17.132 kb on + strand, within GFF1552 at 17.132 kb on + strand, within GFF1552 at 17.308 kb on + strand, within GFF1552 at 17.354 kb on + strand, within GFF1552 at 17.354 kb on + strand, within GFF1552 at 17.354 kb on + strand, within GFF1552 at 17.355 kb on - strand, within GFF1552 at 17.456 kb on + strand at 17.562 kb on + strand at 17.622 kb on + strand, within GFF1553 at 17.623 kb on - strand, within GFF1553 at 17.969 kb on + strand, within GFF1554 at 18.039 kb on + strand, within GFF1554
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 15,720 - GFF1551 0.67 -1.0 15,785 - GFF1551 0.73 +2.1 15,836 - GFF1551 0.77 +0.6 15,929 - GFF1551 0.86 +0.1 15,955 + GFF1551 0.88 -1.2 15,955 + GFF1551 0.88 +1.4 16,170 - +0.7 16,604 + +2.8 16,715 - GFF1552 0.17 +0.3 16,815 + GFF1552 0.28 -1.7 16,816 - GFF1552 0.28 -1.0 16,955 + GFF1552 0.43 +0.8 16,956 - GFF1552 0.43 -1.6 16,959 + GFF1552 0.43 +0.6 16,960 - GFF1552 0.44 -0.9 16,963 + GFF1552 0.44 -1.9 16,964 - GFF1552 0.44 +0.4 17,132 + GFF1552 0.62 -1.3 17,132 + GFF1552 0.62 -2.9 17,308 + GFF1552 0.81 -0.1 17,354 + GFF1552 0.86 +0.4 17,354 + GFF1552 0.86 -0.2 17,354 + GFF1552 0.86 -1.3 17,355 - GFF1552 0.86 -0.8 17,456 + -0.1 17,562 + -0.4 17,622 + GFF1553 0.20 -0.8 17,623 - GFF1553 0.20 +0.8 17,969 + GFF1554 0.21 -1.6 18,039 + GFF1554 0.29 -0.5
Or see this region's nucleotide sequence