Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1533 and GFF1534 are separated by 239 nucleotides GFF1534 and GFF1535 are separated by 529 nucleotides
GFF1533 - Fructokinase (EC 2.7.1.4), at 37,131 to 38,078
GFF1533
GFF1534 - D-galactarate dehydratase (EC 4.2.1.42), at 38,318 to 39,889
GFF1534
GFF1535 - 2-dehydro-3-deoxyglucarate aldolase (EC 4.1.2.20), at 40,419 to 41,189
GFF1535
Position (kb) 38
39
40 Strain fitness (log2 ratio) -1
0
1
2 at 37.598 kb on - strand, within GFF1533 at 37.839 kb on + strand, within GFF1533 at 37.840 kb on - strand, within GFF1533 at 37.840 kb on - strand, within GFF1533 at 37.982 kb on + strand, within GFF1533 at 37.983 kb on - strand, within GFF1533 at 38.003 kb on + strand at 38.076 kb on + strand at 38.080 kb on + strand at 38.090 kb on + strand at 38.104 kb on + strand at 38.105 kb on - strand at 38.115 kb on + strand at 38.423 kb on + strand at 38.424 kb on - strand at 38.489 kb on + strand, within GFF1534 at 38.489 kb on + strand, within GFF1534 at 38.490 kb on - strand, within GFF1534 at 38.496 kb on + strand, within GFF1534 at 38.497 kb on - strand, within GFF1534 at 38.591 kb on + strand, within GFF1534 at 38.597 kb on - strand, within GFF1534 at 38.655 kb on + strand, within GFF1534 at 38.656 kb on - strand, within GFF1534 at 38.656 kb on - strand, within GFF1534 at 38.819 kb on - strand, within GFF1534 at 38.826 kb on + strand, within GFF1534 at 38.826 kb on + strand, within GFF1534 at 38.826 kb on + strand, within GFF1534 at 38.856 kb on - strand, within GFF1534 at 39.136 kb on + strand, within GFF1534 at 39.173 kb on - strand, within GFF1534 at 39.377 kb on + strand, within GFF1534 at 39.378 kb on - strand, within GFF1534 at 39.438 kb on + strand, within GFF1534 at 39.439 kb on - strand, within GFF1534 at 39.439 kb on - strand, within GFF1534 at 39.439 kb on - strand, within GFF1534 at 39.476 kb on - strand, within GFF1534 at 39.522 kb on + strand, within GFF1534 at 39.536 kb on - strand, within GFF1534 at 39.589 kb on + strand, within GFF1534 at 39.590 kb on - strand, within GFF1534 at 40.102 kb on - strand at 40.245 kb on + strand at 40.360 kb on + strand at 40.430 kb on + strand at 40.592 kb on - strand, within GFF1535 at 40.809 kb on - strand, within GFF1535 at 40.862 kb on + strand, within GFF1535 at 40.863 kb on - strand, within GFF1535 at 40.863 kb on - strand, within GFF1535
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 37,598 - GFF1533 0.49 +0.7 37,839 + GFF1533 0.75 -0.3 37,840 - GFF1533 0.75 -0.6 37,840 - GFF1533 0.75 -0.0 37,982 + GFF1533 0.90 -0.4 37,983 - GFF1533 0.90 -0.1 38,003 + -0.3 38,076 + +0.4 38,080 + +1.0 38,090 + -0.5 38,104 + +1.3 38,105 - -0.5 38,115 + +0.1 38,423 + -0.3 38,424 - +0.4 38,489 + GFF1534 0.11 +2.2 38,489 + GFF1534 0.11 +0.4 38,490 - GFF1534 0.11 -0.1 38,496 + GFF1534 0.11 +2.6 38,497 - GFF1534 0.11 -0.6 38,591 + GFF1534 0.17 +0.6 38,597 - GFF1534 0.18 -0.2 38,655 + GFF1534 0.21 +0.1 38,656 - GFF1534 0.22 +0.8 38,656 - GFF1534 0.22 -0.2 38,819 - GFF1534 0.32 -0.3 38,826 + GFF1534 0.32 +0.9 38,826 + GFF1534 0.32 -0.0 38,826 + GFF1534 0.32 +0.3 38,856 - GFF1534 0.34 -0.1 39,136 + GFF1534 0.52 -1.4 39,173 - GFF1534 0.54 -0.6 39,377 + GFF1534 0.67 +0.0 39,378 - GFF1534 0.67 +0.6 39,438 + GFF1534 0.71 +0.1 39,439 - GFF1534 0.71 -0.7 39,439 - GFF1534 0.71 -0.8 39,439 - GFF1534 0.71 -0.5 39,476 - GFF1534 0.74 +0.2 39,522 + GFF1534 0.77 +0.7 39,536 - GFF1534 0.77 +0.6 39,589 + GFF1534 0.81 +0.4 39,590 - GFF1534 0.81 +0.3 40,102 - -0.3 40,245 + -0.1 40,360 + -0.8 40,430 + +1.9 40,592 - GFF1535 0.22 +0.6 40,809 - GFF1535 0.51 +0.5 40,862 + GFF1535 0.57 +0.3 40,863 - GFF1535 0.58 +0.0 40,863 - GFF1535 0.58 +0.3
Or see this region's nucleotide sequence