Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1480 and GFF1481 overlap by 4 nucleotides GFF1481 and GFF1482 are separated by 355 nucleotides
GFF1480 - Fumarate respiration transcriptional regulator DcuR, at 149,308 to 150,027
GFF1480
GFF1481 - Fumarate respiration sensor kinase protein DcuS, at 150,024 to 151,655
GFF1481
GFF1482 - Anaerobic dimethyl sulfoxide reductase chain A (EC 1.8.99.-), at 152,011 to 154,440
GFF1482
Position (kb) 150
151
152 Strain fitness (log2 ratio) -2
-1
0
1
2 at 149.318 kb on + strand at 149.527 kb on + strand, within GFF1480 at 149.527 kb on + strand, within GFF1480 at 149.665 kb on + strand, within GFF1480 at 149.666 kb on - strand, within GFF1480 at 149.735 kb on + strand, within GFF1480 at 149.751 kb on + strand, within GFF1480 at 149.853 kb on + strand, within GFF1480 at 149.855 kb on + strand, within GFF1480 at 149.856 kb on - strand, within GFF1480 at 149.856 kb on - strand, within GFF1480 at 149.970 kb on - strand at 150.013 kb on + strand at 150.060 kb on + strand at 150.265 kb on + strand, within GFF1481 at 150.271 kb on - strand, within GFF1481 at 150.360 kb on - strand, within GFF1481 at 150.381 kb on - strand, within GFF1481 at 150.381 kb on - strand, within GFF1481 at 150.406 kb on - strand, within GFF1481 at 150.515 kb on - strand, within GFF1481 at 150.518 kb on + strand, within GFF1481 at 150.519 kb on - strand, within GFF1481 at 150.536 kb on + strand, within GFF1481 at 150.538 kb on + strand, within GFF1481 at 150.540 kb on - strand, within GFF1481 at 150.555 kb on - strand, within GFF1481 at 150.556 kb on + strand, within GFF1481 at 150.557 kb on - strand, within GFF1481 at 150.557 kb on - strand, within GFF1481 at 150.592 kb on - strand, within GFF1481 at 150.637 kb on + strand, within GFF1481 at 150.687 kb on - strand, within GFF1481 at 150.743 kb on + strand, within GFF1481 at 150.818 kb on + strand, within GFF1481 at 150.915 kb on - strand, within GFF1481 at 151.061 kb on - strand, within GFF1481 at 151.071 kb on + strand, within GFF1481 at 151.107 kb on - strand, within GFF1481 at 151.379 kb on + strand, within GFF1481 at 151.493 kb on + strand at 151.689 kb on - strand at 151.707 kb on - strand at 152.418 kb on + strand, within GFF1482 at 152.559 kb on - strand, within GFF1482 at 152.632 kb on + strand, within GFF1482 at 152.648 kb on - strand, within GFF1482
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 149,318 + +0.5 149,527 + GFF1480 0.30 +2.1 149,527 + GFF1480 0.30 -0.3 149,665 + GFF1480 0.50 -1.3 149,666 - GFF1480 0.50 -1.8 149,735 + GFF1480 0.59 -0.7 149,751 + GFF1480 0.62 -0.1 149,853 + GFF1480 0.76 +0.0 149,855 + GFF1480 0.76 -1.5 149,856 - GFF1480 0.76 +0.1 149,856 - GFF1480 0.76 -1.1 149,970 - -1.1 150,013 + -0.5 150,060 + -0.5 150,265 + GFF1481 0.15 +0.0 150,271 - GFF1481 0.15 -0.0 150,360 - GFF1481 0.21 -0.3 150,381 - GFF1481 0.22 +0.9 150,381 - GFF1481 0.22 +0.3 150,406 - GFF1481 0.23 +0.6 150,515 - GFF1481 0.30 -0.1 150,518 + GFF1481 0.30 -0.0 150,519 - GFF1481 0.30 -0.5 150,536 + GFF1481 0.31 -0.1 150,538 + GFF1481 0.31 -2.4 150,540 - GFF1481 0.32 -0.4 150,555 - GFF1481 0.33 -2.4 150,556 + GFF1481 0.33 +0.4 150,557 - GFF1481 0.33 -0.3 150,557 - GFF1481 0.33 +1.0 150,592 - GFF1481 0.35 -1.2 150,637 + GFF1481 0.38 -0.8 150,687 - GFF1481 0.41 +0.0 150,743 + GFF1481 0.44 -0.3 150,818 + GFF1481 0.49 -0.2 150,915 - GFF1481 0.55 +0.6 151,061 - GFF1481 0.64 -0.3 151,071 + GFF1481 0.64 -0.4 151,107 - GFF1481 0.66 -0.7 151,379 + GFF1481 0.83 +0.4 151,493 + -0.4 151,689 - +0.4 151,707 - -0.1 152,418 + GFF1482 0.17 -0.4 152,559 - GFF1482 0.23 -0.1 152,632 + GFF1482 0.26 +2.2 152,648 - GFF1482 0.26 +0.4
Or see this region's nucleotide sequence