Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1317 and GFF1318 are separated by 86 nucleotides GFF1318 and GFF1319 overlap by 8 nucleotides
GFF1317 - Oxidoreductase, aldo/keto reductase family, at 24,418 to 25,398
GFF1317
GFF1318 - Lysophospholipid transporter LplT, at 25,485 to 26,687
GFF1318
GFF1319 - 2-acylglycerophosphoethanolamine acyltransferase (EC 2.3.1.40) / Acyl-[acyl-carrier-protein] synthetase (EC 6.2.1.20), at 26,680 to 28,839
GFF1319
Position (kb) 25
26
27 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 24.490 kb on - strand at 24.515 kb on - strand at 24.559 kb on + strand, within GFF1317 at 24.560 kb on - strand, within GFF1317 at 24.636 kb on + strand, within GFF1317 at 24.793 kb on + strand, within GFF1317 at 24.794 kb on - strand, within GFF1317 at 24.795 kb on + strand, within GFF1317 at 24.839 kb on + strand, within GFF1317 at 24.849 kb on - strand, within GFF1317 at 24.849 kb on - strand, within GFF1317 at 24.883 kb on - strand, within GFF1317 at 24.883 kb on - strand, within GFF1317 at 24.981 kb on + strand, within GFF1317 at 25.000 kb on + strand, within GFF1317 at 25.031 kb on - strand, within GFF1317 at 25.126 kb on - strand, within GFF1317 at 25.269 kb on + strand, within GFF1317 at 25.269 kb on + strand, within GFF1317 at 25.378 kb on + strand at 25.378 kb on + strand at 25.381 kb on - strand at 25.419 kb on + strand at 25.447 kb on - strand at 25.447 kb on - strand at 25.489 kb on + strand at 25.490 kb on - strand at 25.617 kb on + strand, within GFF1318 at 25.618 kb on - strand, within GFF1318 at 25.620 kb on + strand, within GFF1318 at 25.620 kb on + strand, within GFF1318 at 25.621 kb on - strand, within GFF1318 at 25.763 kb on - strand, within GFF1318 at 26.078 kb on + strand, within GFF1318 at 26.217 kb on + strand, within GFF1318 at 26.326 kb on + strand, within GFF1318 at 26.369 kb on + strand, within GFF1318 at 26.403 kb on - strand, within GFF1318 at 26.503 kb on + strand, within GFF1318 at 26.670 kb on - strand at 26.673 kb on + strand at 26.822 kb on + strand at 26.823 kb on - strand at 26.830 kb on + strand at 26.862 kb on + strand at 26.862 kb on + strand at 27.044 kb on - strand, within GFF1319 at 27.044 kb on - strand, within GFF1319 at 27.062 kb on + strand, within GFF1319 at 27.153 kb on + strand, within GFF1319 at 27.255 kb on + strand, within GFF1319 at 27.309 kb on - strand, within GFF1319 at 27.472 kb on - strand, within GFF1319 at 27.472 kb on - strand, within GFF1319 at 27.477 kb on + strand, within GFF1319 at 27.486 kb on - strand, within GFF1319 at 27.486 kb on - strand, within GFF1319 at 27.536 kb on - strand, within GFF1319 at 27.678 kb on + strand, within GFF1319 at 27.679 kb on - strand, within GFF1319
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 24,490 - -0.2 24,515 - +0.2 24,559 + GFF1317 0.14 +0.3 24,560 - GFF1317 0.14 -0.3 24,636 + GFF1317 0.22 +0.6 24,793 + GFF1317 0.38 +0.4 24,794 - GFF1317 0.38 -0.6 24,795 + GFF1317 0.38 +0.2 24,839 + GFF1317 0.43 -0.6 24,849 - GFF1317 0.44 -0.9 24,849 - GFF1317 0.44 -0.3 24,883 - GFF1317 0.47 -0.5 24,883 - GFF1317 0.47 +0.5 24,981 + GFF1317 0.57 +1.0 25,000 + GFF1317 0.59 +0.9 25,031 - GFF1317 0.62 -1.5 25,126 - GFF1317 0.72 -0.9 25,269 + GFF1317 0.87 -0.1 25,269 + GFF1317 0.87 +1.0 25,378 + -2.5 25,378 + +0.3 25,381 - -3.2 25,419 + -1.7 25,447 - -0.3 25,447 - -1.2 25,489 + +2.5 25,490 - -2.8 25,617 + GFF1318 0.11 +3.1 25,618 - GFF1318 0.11 -0.8 25,620 + GFF1318 0.11 -2.9 25,620 + GFF1318 0.11 +0.4 25,621 - GFF1318 0.11 +0.4 25,763 - GFF1318 0.23 -1.7 26,078 + GFF1318 0.49 -1.8 26,217 + GFF1318 0.61 -1.1 26,326 + GFF1318 0.70 -1.0 26,369 + GFF1318 0.73 -2.9 26,403 - GFF1318 0.76 -0.5 26,503 + GFF1318 0.85 -1.4 26,670 - -1.2 26,673 + -1.0 26,822 + -0.0 26,823 - -2.8 26,830 + -0.1 26,862 + -2.0 26,862 + +2.5 27,044 - GFF1319 0.17 +0.5 27,044 - GFF1319 0.17 -0.4 27,062 + GFF1319 0.18 +0.0 27,153 + GFF1319 0.22 -0.1 27,255 + GFF1319 0.27 -0.8 27,309 - GFF1319 0.29 +0.5 27,472 - GFF1319 0.37 +0.4 27,472 - GFF1319 0.37 -0.0 27,477 + GFF1319 0.37 -1.8 27,486 - GFF1319 0.37 +0.3 27,486 - GFF1319 0.37 -0.1 27,536 - GFF1319 0.40 +0.0 27,678 + GFF1319 0.46 +0.8 27,679 - GFF1319 0.46 -1.2
Or see this region's nucleotide sequence