Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1185 and GFF1186 are separated by 8 nucleotides GFF1186 and GFF1187 overlap by 1 nucleotides GFF1187 and GFF1188 are separated by 78 nucleotides GFF1188 and GFF1189 are separated by 19 nucleotides GFF1189 and GFF1190 are separated by 54 nucleotides
GFF1185 - IncF plasmid conjugative transfer protein TraD, at 30,607 to 32,850
GFF1185
GFF1186 - VapC toxin protein, at 32,859 to 33,257
GFF1186
GFF1187 - VapB protein (antitoxin to VapC), at 33,257 to 33,487
GFF1187
GFF1188 - IncF plasmid conjugative transfer DNA-nicking and unwinding protein TraI, at 33,566 to 38,824
GFF1188
GFF1189 - IncF plasmid conjugative transfer pilin acetylase TraX, at 38,844 to 39,584
GFF1189
GFF1190 - IncF plasmid conjugative transfer fertility inhibition protein FinO, at 39,639 to 40,202
GFF1190
Position (kb) 33
34
35
36
37
38
39 Strain fitness (log2 ratio) -2
-1
0
1
2 at 32.617 kb on - strand, within GFF1185 at 32.691 kb on + strand at 32.725 kb on + strand at 32.793 kb on + strand at 33.044 kb on - strand, within GFF1186 at 33.124 kb on + strand, within GFF1186 at 33.156 kb on - strand, within GFF1186 at 33.233 kb on + strand at 33.234 kb on - strand at 33.234 kb on - strand at 33.236 kb on + strand at 33.246 kb on - strand at 33.304 kb on + strand, within GFF1187 at 33.368 kb on - strand, within GFF1187 at 33.470 kb on + strand at 33.471 kb on - strand at 33.505 kb on + strand at 33.506 kb on - strand at 33.512 kb on + strand at 33.513 kb on - strand at 33.517 kb on - strand at 33.574 kb on + strand at 33.575 kb on - strand at 33.614 kb on + strand at 33.615 kb on - strand at 33.628 kb on + strand at 33.628 kb on + strand at 33.628 kb on + strand at 33.628 kb on + strand at 33.628 kb on + strand at 33.629 kb on - strand at 33.632 kb on + strand at 33.633 kb on - strand at 33.635 kb on + strand at 33.636 kb on - strand at 33.636 kb on - strand at 33.636 kb on - strand at 33.636 kb on - strand at 33.862 kb on + strand at 33.863 kb on - strand at 34.037 kb on - strand at 34.121 kb on - strand, within GFF1188 at 34.133 kb on + strand, within GFF1188 at 34.134 kb on - strand, within GFF1188 at 34.134 kb on - strand, within GFF1188 at 34.134 kb on - strand, within GFF1188 at 34.147 kb on - strand, within GFF1188 at 34.231 kb on + strand, within GFF1188 at 34.231 kb on + strand, within GFF1188 at 34.231 kb on + strand, within GFF1188 at 34.565 kb on - strand, within GFF1188 at 34.565 kb on - strand, within GFF1188 at 34.570 kb on + strand, within GFF1188 at 35.071 kb on + strand, within GFF1188 at 35.161 kb on - strand, within GFF1188 at 35.161 kb on - strand, within GFF1188 at 35.165 kb on - strand, within GFF1188 at 35.403 kb on + strand, within GFF1188 at 35.496 kb on - strand, within GFF1188 at 35.506 kb on + strand, within GFF1188 at 35.506 kb on + strand, within GFF1188 at 35.507 kb on - strand, within GFF1188 at 35.510 kb on + strand, within GFF1188 at 35.510 kb on + strand, within GFF1188 at 35.510 kb on + strand, within GFF1188 at 35.511 kb on - strand, within GFF1188 at 35.567 kb on + strand, within GFF1188 at 35.717 kb on - strand, within GFF1188 at 35.717 kb on - strand, within GFF1188 at 35.773 kb on - strand, within GFF1188 at 35.773 kb on - strand, within GFF1188 at 36.006 kb on - strand, within GFF1188 at 36.312 kb on + strand, within GFF1188 at 36.317 kb on + strand, within GFF1188 at 36.317 kb on + strand, within GFF1188 at 36.318 kb on - strand, within GFF1188 at 36.318 kb on - strand, within GFF1188 at 36.318 kb on - strand, within GFF1188 at 36.371 kb on + strand, within GFF1188 at 36.496 kb on + strand, within GFF1188 at 36.501 kb on + strand, within GFF1188 at 36.531 kb on + strand, within GFF1188 at 36.532 kb on - strand, within GFF1188 at 36.951 kb on + strand, within GFF1188 at 36.952 kb on - strand, within GFF1188 at 37.010 kb on - strand, within GFF1188 at 37.187 kb on + strand, within GFF1188 at 37.209 kb on - strand, within GFF1188 at 37.341 kb on + strand, within GFF1188 at 37.342 kb on - strand, within GFF1188 at 37.342 kb on - strand, within GFF1188 at 37.433 kb on + strand, within GFF1188 at 37.456 kb on + strand, within GFF1188 at 37.486 kb on + strand, within GFF1188 at 37.495 kb on + strand, within GFF1188 at 37.507 kb on + strand, within GFF1188 at 37.507 kb on + strand, within GFF1188 at 37.507 kb on + strand, within GFF1188 at 37.507 kb on + strand, within GFF1188 at 37.508 kb on - strand, within GFF1188 at 37.545 kb on + strand, within GFF1188 at 37.548 kb on - strand, within GFF1188 at 37.619 kb on + strand, within GFF1188 at 37.732 kb on + strand, within GFF1188 at 37.732 kb on + strand, within GFF1188 at 37.850 kb on + strand, within GFF1188 at 37.850 kb on + strand, within GFF1188 at 37.851 kb on - strand, within GFF1188 at 37.851 kb on - strand, within GFF1188 at 38.117 kb on + strand, within GFF1188 at 38.129 kb on + strand, within GFF1188 at 38.130 kb on - strand, within GFF1188 at 38.130 kb on - strand, within GFF1188 at 38.130 kb on - strand, within GFF1188 at 38.173 kb on + strand, within GFF1188 at 38.311 kb on + strand at 38.867 kb on + strand at 38.868 kb on - strand at 38.985 kb on - strand, within GFF1189 at 39.083 kb on + strand, within GFF1189 at 39.083 kb on + strand, within GFF1189 at 39.084 kb on - strand, within GFF1189 at 39.084 kb on - strand, within GFF1189 at 39.171 kb on + strand, within GFF1189 at 39.266 kb on - strand, within GFF1189 at 39.349 kb on - strand, within GFF1189 at 39.475 kb on + strand, within GFF1189 at 39.543 kb on + strand at 39.543 kb on + strand at 39.633 kb on + strand at 39.633 kb on + strand at 39.633 kb on + strand at 39.633 kb on + strand at 39.633 kb on + strand at 39.633 kb on + strand at 39.723 kb on - strand, within GFF1190 at 39.723 kb on - strand, within GFF1190
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 32,617 - GFF1185 0.90 -0.2 32,691 + +0.2 32,725 + -1.3 32,793 + -0.1 33,044 - GFF1186 0.46 -0.6 33,124 + GFF1186 0.66 -0.1 33,156 - GFF1186 0.74 -1.2 33,233 + +0.3 33,234 - +0.3 33,234 - +1.6 33,236 + +0.6 33,246 - -0.6 33,304 + GFF1187 0.20 +0.5 33,368 - GFF1187 0.48 +0.2 33,470 + -1.5 33,471 - +0.8 33,505 + +0.6 33,506 - -0.1 33,512 + +0.3 33,513 - -0.1 33,517 - -1.6 33,574 + +0.5 33,575 - -0.4 33,614 + -1.5 33,615 - -0.5 33,628 + +0.3 33,628 + -0.9 33,628 + -0.3 33,628 + +0.8 33,628 + -1.9 33,629 - +0.2 33,632 + +0.1 33,633 - -0.3 33,635 + +0.2 33,636 - +0.3 33,636 - +0.7 33,636 - +0.5 33,636 - +0.2 33,862 + +0.8 33,863 - -0.7 34,037 - -1.9 34,121 - GFF1188 0.11 +0.7 34,133 + GFF1188 0.11 -0.1 34,134 - GFF1188 0.11 +1.7 34,134 - GFF1188 0.11 -0.0 34,134 - GFF1188 0.11 +0.3 34,147 - GFF1188 0.11 +0.2 34,231 + GFF1188 0.13 +0.0 34,231 + GFF1188 0.13 -0.6 34,231 + GFF1188 0.13 +0.1 34,565 - GFF1188 0.19 +0.1 34,565 - GFF1188 0.19 -0.6 34,570 + GFF1188 0.19 -0.1 35,071 + GFF1188 0.29 -0.7 35,161 - GFF1188 0.30 -1.6 35,161 - GFF1188 0.30 -0.2 35,165 - GFF1188 0.30 -0.0 35,403 + GFF1188 0.35 -0.8 35,496 - GFF1188 0.37 +1.1 35,506 + GFF1188 0.37 -0.2 35,506 + GFF1188 0.37 -0.1 35,507 - GFF1188 0.37 -1.3 35,510 + GFF1188 0.37 +0.1 35,510 + GFF1188 0.37 +0.3 35,510 + GFF1188 0.37 -0.4 35,511 - GFF1188 0.37 +0.4 35,567 + GFF1188 0.38 +0.2 35,717 - GFF1188 0.41 -0.8 35,717 - GFF1188 0.41 -1.3 35,773 - GFF1188 0.42 -0.9 35,773 - GFF1188 0.42 +0.1 36,006 - GFF1188 0.46 -0.4 36,312 + GFF1188 0.52 +0.3 36,317 + GFF1188 0.52 -0.2 36,317 + GFF1188 0.52 +0.3 36,318 - GFF1188 0.52 +0.1 36,318 - GFF1188 0.52 +0.2 36,318 - GFF1188 0.52 -0.0 36,371 + GFF1188 0.53 -0.1 36,496 + GFF1188 0.56 -0.5 36,501 + GFF1188 0.56 -0.0 36,531 + GFF1188 0.56 -1.5 36,532 - GFF1188 0.56 +1.2 36,951 + GFF1188 0.64 +1.9 36,952 - GFF1188 0.64 +0.4 37,010 - GFF1188 0.65 -0.6 37,187 + GFF1188 0.69 -1.3 37,209 - GFF1188 0.69 -0.9 37,341 + GFF1188 0.72 -0.6 37,342 - GFF1188 0.72 -1.0 37,342 - GFF1188 0.72 +0.9 37,433 + GFF1188 0.74 +0.4 37,456 + GFF1188 0.74 +0.0 37,486 + GFF1188 0.75 -0.1 37,495 + GFF1188 0.75 +1.3 37,507 + GFF1188 0.75 +0.0 37,507 + GFF1188 0.75 +0.5 37,507 + GFF1188 0.75 +1.4 37,507 + GFF1188 0.75 -0.2 37,508 - GFF1188 0.75 -0.1 37,545 + GFF1188 0.76 +0.0 37,548 - GFF1188 0.76 +0.1 37,619 + GFF1188 0.77 +1.2 37,732 + GFF1188 0.79 -0.0 37,732 + GFF1188 0.79 +0.4 37,850 + GFF1188 0.81 +0.4 37,850 + GFF1188 0.81 +0.2 37,851 - GFF1188 0.81 +0.7 37,851 - GFF1188 0.81 -0.4 38,117 + GFF1188 0.87 +2.2 38,129 + GFF1188 0.87 +0.5 38,130 - GFF1188 0.87 -0.7 38,130 - GFF1188 0.87 -0.2 38,130 - GFF1188 0.87 +0.0 38,173 + GFF1188 0.88 +0.2 38,311 + +1.4 38,867 + +0.9 38,868 - +0.1 38,985 - GFF1189 0.19 -1.7 39,083 + GFF1189 0.32 -1.5 39,083 + GFF1189 0.32 +0.2 39,084 - GFF1189 0.32 +0.4 39,084 - GFF1189 0.32 -0.9 39,171 + GFF1189 0.44 -0.3 39,266 - GFF1189 0.57 +0.8 39,349 - GFF1189 0.68 -0.4 39,475 + GFF1189 0.85 +0.0 39,543 + +0.1 39,543 + +0.1 39,633 + +0.2 39,633 + -0.3 39,633 + +0.5 39,633 + +0.1 39,633 + +0.0 39,633 + -1.6 39,723 - GFF1190 0.15 -0.9 39,723 - GFF1190 0.15 -1.1
Or see this region's nucleotide sequence