Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF852

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF850 and GFF851 are separated by 9 nucleotidesGFF851 and GFF852 overlap by 1 nucleotidesGFF852 and GFF853 are separated by 128 nucleotidesGFF853 and GFF854 are separated by 470 nucleotides GFF850 - 3-keto-L-gulonate-6-phosphate decarboxylase UlaD (EC 4.1.1.85) (L-ascorbate utilization protein D), at 30,788 to 31,438 GFF850 GFF851 - L-ribulose-5-phosphate 3-epimerase UlaE (EC 5.1.3.22) (L-ascorbate utilization protein E), at 31,448 to 32,302 GFF851 GFF852 - L-ribulose-5-phosphate 4-epimerase UlaF (EC 5.1.3.4) (L-ascorbate utilization protein F), at 32,302 to 32,988 GFF852 GFF853 - UPF0379 protein yjfY precursor, at 33,117 to 33,392 GFF853 GFF854 - SSU ribosomal protein S6p, at 33,863 to 34,258 GFF854 Position (kb) 32 33Strain fitness (log2 ratio) -2 -1 0 1at 31.741 kb on + strand, within GFF851at 31.748 kb on - strand, within GFF851at 31.811 kb on + strand, within GFF851at 31.811 kb on + strand, within GFF851at 31.815 kb on - strand, within GFF851at 31.861 kb on - strand, within GFF851at 31.946 kb on - strand, within GFF851at 31.967 kb on + strand, within GFF851at 31.968 kb on - strand, within GFF851at 31.976 kb on - strand, within GFF851at 32.034 kb on + strand, within GFF851at 32.089 kb on + strand, within GFF851at 32.093 kb on - strand, within GFF851at 32.189 kb on + strand, within GFF851at 32.325 kb on - strandat 32.325 kb on - strandat 32.370 kb on + strandat 32.422 kb on - strand, within GFF852at 32.457 kb on - strand, within GFF852at 32.479 kb on - strand, within GFF852at 32.545 kb on + strand, within GFF852at 32.716 kb on + strand, within GFF852at 32.785 kb on - strand, within GFF852at 32.841 kb on - strand, within GFF852at 32.845 kb on - strand, within GFF852at 32.926 kb on + strandat 32.926 kb on + strandat 32.953 kb on - strandat 33.012 kb on - strandat 33.125 kb on - strandat 33.128 kb on + strandat 33.178 kb on - strand, within GFF853at 33.178 kb on - strand, within GFF853at 33.188 kb on - strand, within GFF853at 33.188 kb on - strand, within GFF853at 33.190 kb on + strand, within GFF853at 33.190 kb on + strand, within GFF853at 33.191 kb on - strand, within GFF853at 33.258 kb on - strand, within GFF853at 33.318 kb on - strand, within GFF853at 33.392 kb on + strandat 33.398 kb on - strandat 33.562 kb on - strandat 33.562 kb on - strandat 33.562 kb on - strandat 33.576 kb on - strandat 33.666 kb on + strandat 33.667 kb on - strandat 33.669 kb on + strandat 33.703 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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31,741 + GFF851 0.34 +0.2
31,748 - GFF851 0.35 +0.1
31,811 + GFF851 0.42 -0.2
31,811 + GFF851 0.42 +0.3
31,815 - GFF851 0.43 -0.2
31,861 - GFF851 0.48 +0.5
31,946 - GFF851 0.58 -0.0
31,967 + GFF851 0.61 -0.8
31,968 - GFF851 0.61 +0.2
31,976 - GFF851 0.62 +0.2
32,034 + GFF851 0.69 +0.1
32,089 + GFF851 0.75 +0.5
32,093 - GFF851 0.75 -0.1
32,189 + GFF851 0.87 +0.6
32,325 - -1.3
32,325 - +0.4
32,370 + -0.0
32,422 - GFF852 0.17 -1.8
32,457 - GFF852 0.23 -0.5
32,479 - GFF852 0.26 -0.8
32,545 + GFF852 0.35 -0.1
32,716 + GFF852 0.60 -0.1
32,785 - GFF852 0.70 -0.8
32,841 - GFF852 0.78 -0.3
32,845 - GFF852 0.79 -0.2
32,926 + -0.6
32,926 + -0.0
32,953 - +0.1
33,012 - -0.0
33,125 - -0.3
33,128 + -0.9
33,178 - GFF853 0.22 +0.3
33,178 - GFF853 0.22 -0.3
33,188 - GFF853 0.26 -0.6
33,188 - GFF853 0.26 +0.5
33,190 + GFF853 0.26 -0.5
33,190 + GFF853 0.26 +1.0
33,191 - GFF853 0.27 +0.5
33,258 - GFF853 0.51 -0.7
33,318 - GFF853 0.73 +0.2
33,392 + -0.6
33,398 - -0.8
33,562 - -0.2
33,562 - -0.6
33,562 - -0.3
33,576 - -0.0
33,666 + -2.7
33,667 - -1.3
33,669 + -0.3
33,703 - +0.4

Or see this region's nucleotide sequence