Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF749 and GFF750 are separated by 167 nucleotides GFF750 and GFF751 are separated by 116 nucleotides GFF751 and GFF752 are separated by 477 nucleotides GFF752 and GFF753 are separated by 31 nucleotides
GFF749 - Putative transport protein/putative regulator, at 107,657 to 108,868
GFF749
GFF750 - Transcriptional regulator, TetR family, at 109,036 to 109,656
GFF750
GFF751 - TrkA, Potassium channel-family protein, at 109,773 to 111,326
GFF751
GFF752 - Inner membrane protein, at 111,804 to 112,106
GFF752
GFF753 - Glutaredoxin 1, at 112,138 to 112,401
GFF753
Position (kb)
109
110
111
112 Strain fitness (log2 ratio)
-2
-1
0
1 at 108.814 kb on + strand at 108.814 kb on + strand at 108.814 kb on + strand at 108.868 kb on + strand at 108.906 kb on + strand at 108.942 kb on + strand at 108.943 kb on - strand at 108.997 kb on + strand at 109.071 kb on + strand at 109.193 kb on - strand, within GFF750 at 109.369 kb on + strand, within GFF750 at 109.376 kb on + strand, within GFF750 at 109.376 kb on + strand, within GFF750 at 109.376 kb on + strand, within GFF750 at 109.376 kb on + strand, within GFF750 at 109.377 kb on - strand, within GFF750 at 109.377 kb on - strand, within GFF750 at 109.700 kb on - strand at 109.700 kb on - strand at 109.734 kb on + strand at 109.822 kb on - strand at 109.973 kb on - strand, within GFF751 at 109.973 kb on - strand, within GFF751 at 110.102 kb on + strand, within GFF751 at 110.136 kb on - strand, within GFF751 at 110.155 kb on + strand, within GFF751 at 110.166 kb on - strand, within GFF751 at 110.166 kb on - strand, within GFF751 at 110.262 kb on - strand, within GFF751 at 110.262 kb on - strand, within GFF751 at 110.328 kb on - strand, within GFF751 at 110.395 kb on - strand, within GFF751 at 110.561 kb on + strand, within GFF751 at 110.562 kb on - strand, within GFF751 at 110.583 kb on + strand, within GFF751 at 110.700 kb on - strand, within GFF751 at 110.725 kb on + strand, within GFF751 at 110.907 kb on + strand, within GFF751 at 110.959 kb on + strand, within GFF751 at 110.987 kb on + strand, within GFF751 at 111.126 kb on - strand, within GFF751 at 111.260 kb on + strand at 111.313 kb on - strand at 111.585 kb on + strand at 111.611 kb on + strand at 111.611 kb on + strand at 111.803 kb on + strand at 111.804 kb on - strand at 111.811 kb on + strand at 111.811 kb on + strand at 111.812 kb on - strand at 111.812 kb on - strand at 112.015 kb on + strand, within GFF752 at 112.234 kb on + strand, within GFF753 at 112.296 kb on + strand, within GFF753
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 108,814 + +1.2 108,814 + +0.9 108,814 + +0.3 108,868 + +1.5 108,906 + +1.3 108,942 + +0.2 108,943 - +0.9 108,997 + +0.6 109,071 + -0.3 109,193 - GFF750 0.25 -0.9 109,369 + GFF750 0.54 -0.1 109,376 + GFF750 0.55 -2.1 109,376 + GFF750 0.55 -0.5 109,376 + GFF750 0.55 +0.2 109,376 + GFF750 0.55 -0.1 109,377 - GFF750 0.55 -1.5 109,377 - GFF750 0.55 -0.0 109,700 - -0.1 109,700 - +0.4 109,734 + +0.4 109,822 - +0.2 109,973 - GFF751 0.13 +0.7 109,973 - GFF751 0.13 -0.2 110,102 + GFF751 0.21 +0.1 110,136 - GFF751 0.23 +1.6 110,155 + GFF751 0.25 +0.4 110,166 - GFF751 0.25 +0.4 110,166 - GFF751 0.25 +0.1 110,262 - GFF751 0.31 +0.6 110,262 - GFF751 0.31 +0.4 110,328 - GFF751 0.36 +0.5 110,395 - GFF751 0.40 +0.8 110,561 + GFF751 0.51 +0.7 110,562 - GFF751 0.51 +0.3 110,583 + GFF751 0.52 +0.3 110,700 - GFF751 0.60 -0.2 110,725 + GFF751 0.61 +0.9 110,907 + GFF751 0.73 +0.4 110,959 + GFF751 0.76 -1.3 110,987 + GFF751 0.78 +0.7 111,126 - GFF751 0.87 -1.1 111,260 + -0.2 111,313 - -0.1 111,585 + -0.2 111,611 + +0.4 111,611 + -0.2 111,803 + -1.2 111,804 - -0.6 111,811 + +0.6 111,811 + -0.1 111,812 - -0.8 111,812 - +0.9 112,015 + GFF752 0.70 +0.2 112,234 + GFF753 0.36 +0.5 112,296 + GFF753 0.60 +1.2
Or see this region's nucleotide sequence