Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF733

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF731 and GFF732 are separated by 26 nucleotidesGFF732 and GFF733 are separated by 69 nucleotidesGFF733 and GFF734 are separated by 712 nucleotides GFF731 - Ribosomal protein S12p Asp88 (E. coli) methylthiotransferase, at 90,593 to 91,918 GFF731 GFF732 - hypothetical protein, at 91,945 to 92,079 GFF732 GFF733 - biofilm regulator BssR, at 92,149 to 92,532 GFF733 GFF734 - putative cytoplasmic protein, at 93,245 to 93,760 GFF734 Position (kb) 92 93Strain fitness (log2 ratio) -2 -1 0 1at 91.210 kb on - strand, within GFF731at 91.317 kb on - strand, within GFF731at 91.333 kb on + strand, within GFF731at 91.334 kb on - strand, within GFF731at 91.395 kb on - strand, within GFF731at 91.497 kb on + strand, within GFF731at 91.517 kb on + strand, within GFF731at 91.580 kb on + strand, within GFF731at 91.602 kb on + strand, within GFF731at 91.603 kb on - strand, within GFF731at 91.765 kb on + strand, within GFF731at 91.766 kb on - strand, within GFF731at 92.148 kb on + strandat 92.218 kb on + strand, within GFF733at 92.245 kb on + strand, within GFF733at 92.247 kb on + strand, within GFF733at 92.247 kb on + strand, within GFF733at 92.248 kb on - strand, within GFF733at 92.248 kb on - strand, within GFF733at 92.787 kb on - strandat 92.923 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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91,210 - GFF731 0.47 -1.1
91,317 - GFF731 0.55 +0.7
91,333 + GFF731 0.56 +0.6
91,334 - GFF731 0.56 +0.8
91,395 - GFF731 0.60 -0.1
91,497 + GFF731 0.68 +0.4
91,517 + GFF731 0.70 -0.8
91,580 + GFF731 0.74 -0.0
91,602 + GFF731 0.76 -0.2
91,603 - GFF731 0.76 +0.2
91,765 + GFF731 0.88 +0.2
91,766 - GFF731 0.88 -0.0
92,148 + +0.2
92,218 + GFF733 0.18 +0.0
92,245 + GFF733 0.25 -0.2
92,247 + GFF733 0.26 +0.8
92,247 + GFF733 0.26 +1.1
92,248 - GFF733 0.26 -0.4
92,248 - GFF733 0.26 -2.1
92,787 - -0.2
92,923 + -0.2

Or see this region's nucleotide sequence