Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF612 and GFF613 are separated by 225 nucleotides GFF613 and GFF614 are separated by 5 nucleotides GFF614 and GFF615 are separated by 74 nucleotides
GFF612 - Transcription elongation factor GreB, at 352,055 to 352,528
GFF612
GFF613 - Two-component system response regulator OmpR, at 352,754 to 353,473
GFF613
GFF614 - Osmolarity sensory histidine kinase EnvZ, at 353,479 to 354,822
GFF614
GFF615 - Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49), at 354,897 to 356,516
GFF615
Position (kb) 353
354
355 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 352.540 kb on - strand at 352.571 kb on - strand at 352.572 kb on + strand at 352.599 kb on - strand at 352.713 kb on + strand at 352.714 kb on - strand at 352.721 kb on + strand at 352.721 kb on + strand at 352.721 kb on + strand at 352.747 kb on - strand at 352.792 kb on + strand at 352.912 kb on - strand, within GFF613 at 352.912 kb on - strand, within GFF613 at 352.921 kb on - strand, within GFF613 at 352.993 kb on + strand, within GFF613 at 353.057 kb on + strand, within GFF613 at 353.112 kb on + strand, within GFF613 at 353.303 kb on - strand, within GFF613 at 353.417 kb on - strand at 353.608 kb on - strand at 353.608 kb on - strand at 353.782 kb on + strand, within GFF614 at 353.783 kb on - strand, within GFF614 at 353.793 kb on - strand, within GFF614 at 354.012 kb on - strand, within GFF614 at 354.056 kb on + strand, within GFF614 at 354.070 kb on - strand, within GFF614 at 354.135 kb on + strand, within GFF614 at 354.321 kb on + strand, within GFF614 at 354.322 kb on - strand, within GFF614 at 354.426 kb on + strand, within GFF614 at 354.499 kb on - strand, within GFF614 at 354.660 kb on + strand, within GFF614 at 354.820 kb on + strand at 354.820 kb on + strand at 354.821 kb on - strand at 354.833 kb on + strand at 354.833 kb on + strand at 354.833 kb on + strand at 354.833 kb on + strand at 354.836 kb on + strand at 354.836 kb on + strand at 354.947 kb on + strand at 355.021 kb on + strand at 355.157 kb on + strand, within GFF615 at 355.172 kb on + strand, within GFF615 at 355.173 kb on - strand, within GFF615 at 355.211 kb on - strand, within GFF615 at 355.235 kb on + strand, within GFF615 at 355.236 kb on - strand, within GFF615 at 355.258 kb on + strand, within GFF615 at 355.258 kb on + strand, within GFF615 at 355.259 kb on - strand, within GFF615 at 355.352 kb on + strand, within GFF615 at 355.504 kb on + strand, within GFF615 at 355.504 kb on + strand, within GFF615 at 355.505 kb on - strand, within GFF615
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 352,540 - -0.4 352,571 - +0.0 352,572 + -0.5 352,599 - -2.0 352,713 + +0.4 352,714 - +2.1 352,721 + +0.3 352,721 + +0.1 352,721 + -0.2 352,747 - +2.2 352,792 + +2.4 352,912 - GFF613 0.22 +1.6 352,912 - GFF613 0.22 +3.2 352,921 - GFF613 0.23 +2.5 352,993 + GFF613 0.33 +2.3 353,057 + GFF613 0.42 +2.2 353,112 + GFF613 0.50 +1.8 353,303 - GFF613 0.76 +2.3 353,417 - +3.0 353,608 - +2.3 353,608 - +2.5 353,782 + GFF614 0.23 +2.5 353,783 - GFF614 0.23 +2.3 353,793 - GFF614 0.23 +2.2 354,012 - GFF614 0.40 +1.0 354,056 + GFF614 0.43 +2.9 354,070 - GFF614 0.44 +2.7 354,135 + GFF614 0.49 +2.2 354,321 + GFF614 0.63 +2.5 354,322 - GFF614 0.63 +3.4 354,426 + GFF614 0.70 +2.2 354,499 - GFF614 0.76 +2.4 354,660 + GFF614 0.88 +1.9 354,820 + +1.1 354,820 + -0.1 354,821 - -0.4 354,833 + -0.5 354,833 + +0.3 354,833 + -0.0 354,833 + -0.1 354,836 + -1.2 354,836 + +1.6 354,947 + +0.1 355,021 + -0.2 355,157 + GFF615 0.16 +1.2 355,172 + GFF615 0.17 -0.1 355,173 - GFF615 0.17 +0.5 355,211 - GFF615 0.19 -0.0 355,235 + GFF615 0.21 -0.0 355,236 - GFF615 0.21 +0.4 355,258 + GFF615 0.22 +0.6 355,258 + GFF615 0.22 -0.3 355,259 - GFF615 0.22 -1.0 355,352 + GFF615 0.28 +0.1 355,504 + GFF615 0.37 +0.3 355,504 + GFF615 0.37 +0.2 355,505 - GFF615 0.38 +0.2
Or see this region's nucleotide sequence