Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF563 and GFF564 are separated by 53 nucleotides GFF564 and GFF565 are separated by 15 nucleotides GFF565 and GFF566 are separated by 67 nucleotides GFF566 and GFF567 are separated by 80 nucleotides
GFF563 - Putative inner membrane protein, at 293,692 to 294,663
GFF563
GFF564 - FIG074102: hypothetical protein, at 294,717 to 295,478
GFF564
GFF565 - Putative ribokinase, at 295,494 to 296,708
GFF565
GFF566 - hypothetical protein, at 296,776 to 296,892
GFF566
GFF567 - Putative acetyltransferase, at 296,973 to 297,461
GFF567
Position (kb) 295
296
297 Strain fitness (log2 ratio) -1
0
1 at 294.532 kb on + strand, within GFF563 at 294.547 kb on - strand, within GFF563 at 294.647 kb on + strand at 294.648 kb on - strand at 294.676 kb on - strand at 294.780 kb on - strand at 295.478 kb on + strand at 295.502 kb on + strand at 295.536 kb on - strand at 295.568 kb on - strand at 295.704 kb on + strand, within GFF565 at 295.782 kb on - strand, within GFF565 at 296.160 kb on - strand, within GFF565 at 296.494 kb on + strand, within GFF565 at 296.750 kb on - strand at 296.904 kb on + strand at 296.904 kb on + strand at 297.069 kb on - strand, within GFF567 at 297.071 kb on - strand, within GFF567 at 297.116 kb on + strand, within GFF567 at 297.261 kb on - strand, within GFF567 at 297.308 kb on + strand, within GFF567 at 297.370 kb on - strand, within GFF567 at 297.383 kb on - strand, within GFF567 at 297.467 kb on - strand at 297.490 kb on - strand at 297.699 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 294,532 + GFF563 0.86 -1.7 294,547 - GFF563 0.88 -0.2 294,647 + -0.0 294,648 - -0.4 294,676 - -1.2 294,780 - +0.4 295,478 + -0.8 295,502 + +0.7 295,536 - -1.3 295,568 - -0.3 295,704 + GFF565 0.17 -0.0 295,782 - GFF565 0.24 -0.8 296,160 - GFF565 0.55 -0.5 296,494 + GFF565 0.82 -0.1 296,750 - -0.3 296,904 + +0.2 296,904 + -0.2 297,069 - GFF567 0.20 -1.0 297,071 - GFF567 0.20 -0.5 297,116 + GFF567 0.29 +0.3 297,261 - GFF567 0.59 -0.1 297,308 + GFF567 0.69 -0.3 297,370 - GFF567 0.81 +0.0 297,383 - GFF567 0.84 -0.6 297,467 - +1.1 297,490 - +0.2 297,699 + +0.0
Or see this region's nucleotide sequence