Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF562 and GFF563 overlap by 4 nucleotides GFF563 and GFF564 are separated by 53 nucleotides GFF564 and GFF565 are separated by 15 nucleotides
GFF562 - Puative phophotriesterase, at 292,661 to 293,695
GFF562
GFF563 - Putative inner membrane protein, at 293,692 to 294,663
GFF563
GFF564 - FIG074102: hypothetical protein, at 294,717 to 295,478
GFF564
GFF565 - Putative ribokinase, at 295,494 to 296,708
GFF565
Position (kb) 293
294
295 Strain fitness (log2 ratio) -1
0
1 at 292.694 kb on - strand at 292.712 kb on - strand at 292.741 kb on - strand at 292.823 kb on + strand, within GFF562 at 292.953 kb on - strand, within GFF562 at 292.972 kb on + strand, within GFF562 at 293.104 kb on + strand, within GFF562 at 293.129 kb on - strand, within GFF562 at 293.129 kb on - strand, within GFF562 at 293.162 kb on - strand, within GFF562 at 293.254 kb on + strand, within GFF562 at 293.287 kb on + strand, within GFF562 at 293.288 kb on - strand, within GFF562 at 293.319 kb on + strand, within GFF562 at 293.443 kb on + strand, within GFF562 at 293.580 kb on + strand, within GFF562 at 293.580 kb on + strand, within GFF562 at 293.673 kb on - strand at 293.735 kb on + strand at 293.802 kb on - strand, within GFF563 at 293.896 kb on + strand, within GFF563 at 294.061 kb on - strand, within GFF563 at 294.061 kb on - strand, within GFF563 at 294.061 kb on - strand, within GFF563 at 294.532 kb on + strand, within GFF563 at 294.547 kb on - strand, within GFF563 at 294.647 kb on + strand at 294.648 kb on - strand at 294.676 kb on - strand at 294.780 kb on - strand at 295.478 kb on + strand at 295.502 kb on + strand at 295.536 kb on - strand at 295.568 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 292,694 - -0.5 292,712 - -0.3 292,741 - -0.6 292,823 + GFF562 0.16 -0.4 292,953 - GFF562 0.28 -1.1 292,972 + GFF562 0.30 -0.5 293,104 + GFF562 0.43 -0.3 293,129 - GFF562 0.45 -0.5 293,129 - GFF562 0.45 -0.4 293,162 - GFF562 0.48 +0.1 293,254 + GFF562 0.57 +0.0 293,287 + GFF562 0.60 -1.4 293,288 - GFF562 0.61 -0.8 293,319 + GFF562 0.64 -0.8 293,443 + GFF562 0.76 +0.9 293,580 + GFF562 0.89 -0.9 293,580 + GFF562 0.89 -0.1 293,673 - +0.2 293,735 + +0.7 293,802 - GFF563 0.11 -0.8 293,896 + GFF563 0.21 -0.4 294,061 - GFF563 0.38 +0.0 294,061 - GFF563 0.38 -0.1 294,061 - GFF563 0.38 -1.2 294,532 + GFF563 0.86 -1.7 294,547 - GFF563 0.88 -0.2 294,647 + -0.0 294,648 - -0.4 294,676 - -1.2 294,780 - +0.4 295,478 + -0.8 295,502 + +0.7 295,536 - -1.3 295,568 - -0.3
Or see this region's nucleotide sequence