Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4845 and GFF4846 are separated by 18 nucleotides GFF4846 and GFF4847 overlap by 1 nucleotides GFF4847 and GFF4848 are separated by 611 nucleotides
GFF4845 - Hypothetical flavoprotein YqcA (clustered with tRNA pseudouridine synthase C), at 145,042 to 145,491
GFF4845
GFF4846 - tRNA pseudouridine synthase C (EC 4.2.1.70), at 145,510 to 146,292
GFF4846
GFF4847 - Hypothetical protein YqcC (clustered with tRNA pseudouridine synthase C), at 146,292 to 146,621
GFF4847
GFF4848 - Syd protein, at 147,233 to 147,778
GFF4848
Position (kb)
146
147 Strain fitness (log2 ratio)
-2
-1
0
1 at 145.344 kb on + strand, within GFF4845 at 145.344 kb on + strand, within GFF4845 at 145.458 kb on - strand at 145.458 kb on - strand at 145.458 kb on - strand at 145.492 kb on + strand at 145.493 kb on - strand at 145.512 kb on - strand at 145.770 kb on + strand, within GFF4846 at 146.277 kb on - strand at 146.300 kb on + strand at 146.389 kb on + strand, within GFF4847 at 146.390 kb on - strand, within GFF4847 at 146.447 kb on + strand, within GFF4847 at 146.447 kb on + strand, within GFF4847 at 146.447 kb on + strand, within GFF4847 at 146.448 kb on - strand, within GFF4847 at 146.448 kb on - strand, within GFF4847 at 146.448 kb on - strand, within GFF4847 at 146.585 kb on + strand, within GFF4847 at 146.585 kb on + strand, within GFF4847 at 146.586 kb on - strand, within GFF4847 at 146.586 kb on - strand, within GFF4847 at 146.586 kb on - strand, within GFF4847 at 146.953 kb on - strand at 147.032 kb on - strand at 147.057 kb on - strand at 147.289 kb on + strand, within GFF4848 at 147.480 kb on - strand, within GFF4848 at 147.484 kb on + strand, within GFF4848 at 147.560 kb on + strand, within GFF4848 at 147.561 kb on - strand, within GFF4848 at 147.561 kb on - strand, within GFF4848 at 147.566 kb on - strand, within GFF4848
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 145,344 + GFF4845 0.67 -0.0 145,344 + GFF4845 0.67 -0.9 145,458 - +0.4 145,458 - -1.5 145,458 - -0.5 145,492 + -0.4 145,493 - -1.6 145,512 - -0.3 145,770 + GFF4846 0.33 -0.2 146,277 - -0.6 146,300 + +0.2 146,389 + GFF4847 0.29 +0.3 146,390 - GFF4847 0.30 -1.0 146,447 + GFF4847 0.47 -0.7 146,447 + GFF4847 0.47 -1.4 146,447 + GFF4847 0.47 -0.1 146,448 - GFF4847 0.47 -0.6 146,448 - GFF4847 0.47 -0.9 146,448 - GFF4847 0.47 -0.1 146,585 + GFF4847 0.89 -1.5 146,585 + GFF4847 0.89 -1.4 146,586 - GFF4847 0.89 -0.6 146,586 - GFF4847 0.89 -0.7 146,586 - GFF4847 0.89 +0.2 146,953 - -2.5 147,032 - -1.4 147,057 - -1.4 147,289 + GFF4848 0.10 +0.5 147,480 - GFF4848 0.45 -0.1 147,484 + GFF4848 0.46 +0.6 147,560 + GFF4848 0.60 +0.2 147,561 - GFF4848 0.60 -0.3 147,561 - GFF4848 0.60 -0.0 147,566 - GFF4848 0.61 -0.7
Or see this region's nucleotide sequence