Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4694 and GFF4695 are separated by 477 nucleotides GFF4695 and GFF4696 are separated by 253 nucleotides GFF4696 and GFF4697 overlap by 4 nucleotides
GFF4694 - C-terminal domain of CinA type S; Protein Implicated in DNA repair function with RecA and MutS, at 5,780 to 6,277
GFF4694
GFF4695 - Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-), at 6,755 to 7,834
GFF4695
GFF4696 - PTS system, glucitol/sorbitol-specific IIC component (EC 2.7.1.69), at 8,088 to 8,651
GFF4696
GFF4697 - PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components (EC 2.7.1.69), at 8,648 to 9,619
GFF4697
Position (kb)
6
7
8 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5.823 kb on + strand at 5.972 kb on - strand, within GFF4694 at 6.032 kb on + strand, within GFF4694 at 6.107 kb on - strand, within GFF4694 at 6.107 kb on - strand, within GFF4694 at 6.110 kb on - strand, within GFF4694 at 6.110 kb on - strand, within GFF4694 at 6.110 kb on - strand, within GFF4694 at 6.110 kb on - strand, within GFF4694 at 6.112 kb on - strand, within GFF4694 at 6.112 kb on - strand, within GFF4694 at 6.356 kb on - strand at 6.393 kb on - strand at 6.410 kb on + strand at 6.527 kb on + strand at 6.555 kb on - strand at 6.555 kb on - strand at 6.654 kb on + strand at 6.655 kb on - strand at 6.655 kb on - strand at 6.719 kb on - strand at 6.843 kb on + strand at 6.941 kb on + strand, within GFF4695 at 7.066 kb on - strand, within GFF4695 at 7.162 kb on + strand, within GFF4695 at 7.163 kb on - strand, within GFF4695 at 7.163 kb on - strand, within GFF4695 at 7.292 kb on - strand, within GFF4695 at 7.339 kb on + strand, within GFF4695 at 7.339 kb on + strand, within GFF4695 at 7.340 kb on - strand, within GFF4695 at 7.340 kb on - strand, within GFF4695 at 7.340 kb on - strand, within GFF4695 at 7.399 kb on - strand, within GFF4695 at 7.401 kb on - strand, within GFF4695 at 7.401 kb on - strand, within GFF4695 at 7.566 kb on + strand, within GFF4695 at 7.567 kb on - strand, within GFF4695 at 7.567 kb on - strand, within GFF4695 at 7.579 kb on + strand, within GFF4695 at 7.627 kb on + strand, within GFF4695 at 7.627 kb on + strand, within GFF4695 at 7.640 kb on - strand, within GFF4695 at 7.699 kb on + strand, within GFF4695 at 7.876 kb on - strand at 7.915 kb on + strand at 8.102 kb on + strand at 8.174 kb on + strand, within GFF4696 at 8.289 kb on + strand, within GFF4696 at 8.290 kb on - strand, within GFF4696 at 8.299 kb on + strand, within GFF4696 at 8.383 kb on - strand, within GFF4696 at 8.386 kb on - strand, within GFF4696 at 8.400 kb on + strand, within GFF4696 at 8.435 kb on + strand, within GFF4696 at 8.435 kb on + strand, within GFF4696 at 8.524 kb on - strand, within GFF4696 at 8.534 kb on - strand, within GFF4696 at 8.534 kb on - strand, within GFF4696 at 8.711 kb on - strand at 8.732 kb on + strand at 8.732 kb on + strand at 8.734 kb on + strand at 8.734 kb on + strand at 8.735 kb on - strand at 8.735 kb on - strand at 8.781 kb on + strand, within GFF4697
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 5,823 + -0.5 5,972 - GFF4694 0.39 -0.2 6,032 + GFF4694 0.51 -1.0 6,107 - GFF4694 0.66 -1.0 6,107 - GFF4694 0.66 -1.0 6,110 - GFF4694 0.66 -0.2 6,110 - GFF4694 0.66 -0.7 6,110 - GFF4694 0.66 +0.0 6,110 - GFF4694 0.66 +0.2 6,112 - GFF4694 0.67 +1.6 6,112 - GFF4694 0.67 +0.6 6,356 - -0.8 6,393 - +0.7 6,410 + -0.0 6,527 + -1.3 6,555 - -0.7 6,555 - -0.3 6,654 + -1.1 6,655 - -0.3 6,655 - -0.9 6,719 - -0.1 6,843 + -0.9 6,941 + GFF4695 0.17 -0.0 7,066 - GFF4695 0.29 -0.3 7,162 + GFF4695 0.38 -0.9 7,163 - GFF4695 0.38 +0.4 7,163 - GFF4695 0.38 -0.8 7,292 - GFF4695 0.50 -0.1 7,339 + GFF4695 0.54 +0.8 7,339 + GFF4695 0.54 -0.6 7,340 - GFF4695 0.54 -1.0 7,340 - GFF4695 0.54 -0.9 7,340 - GFF4695 0.54 +0.4 7,399 - GFF4695 0.60 -0.2 7,401 - GFF4695 0.60 +0.4 7,401 - GFF4695 0.60 -1.9 7,566 + GFF4695 0.75 -1.5 7,567 - GFF4695 0.75 -0.1 7,567 - GFF4695 0.75 -0.7 7,579 + GFF4695 0.76 -0.5 7,627 + GFF4695 0.81 -1.4 7,627 + GFF4695 0.81 -0.6 7,640 - GFF4695 0.82 -0.2 7,699 + GFF4695 0.87 -0.3 7,876 - +0.1 7,915 + -0.6 8,102 + +0.4 8,174 + GFF4696 0.15 +0.0 8,289 + GFF4696 0.36 +1.1 8,290 - GFF4696 0.36 +0.5 8,299 + GFF4696 0.37 -0.2 8,383 - GFF4696 0.52 +0.4 8,386 - GFF4696 0.53 -1.0 8,400 + GFF4696 0.55 +0.1 8,435 + GFF4696 0.62 +0.2 8,435 + GFF4696 0.62 +0.4 8,524 - GFF4696 0.77 -0.0 8,534 - GFF4696 0.79 +0.8 8,534 - GFF4696 0.79 +0.2 8,711 - +0.7 8,732 + -0.0 8,732 + +0.0 8,734 + -0.9 8,734 + +0.7 8,735 - +0.5 8,735 - +2.5 8,781 + GFF4697 0.14 +0.1
Or see this region's nucleotide sequence