Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4669 and GFF4670 are separated by 207 nucleotides GFF4670 and GFF4671 are separated by 61 nucleotides GFF4671 and GFF4672 overlap by 4 nucleotides GFF4672 and GFF4673 overlap by 4 nucleotides
GFF4669 - Phage outer membrane lytic protein Rz; Endopeptidase (EC 3.4.-.-), at 9 to 473
GFF4669
GFF4670 - FIG01049225: hypothetical protein, at 681 to 1,214
GFF4670
GFF4671 - Phage terminase, large subunit, at 1,276 to 3,309
GFF4671
GFF4672 - FIG01047026: hypothetical protein, at 3,306 to 3,512
GFF4672
GFF4673 - Phage portal protein, at 3,509 to 5,056
GFF4673
Position (kb)
1
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1 at 0.458 kb on + strand at 0.660 kb on - strand at 0.709 kb on + strand at 0.800 kb on + strand, within GFF4670 at 0.804 kb on - strand, within GFF4670 at 0.935 kb on + strand, within GFF4670 at 1.137 kb on + strand, within GFF4670 at 1.138 kb on - strand, within GFF4670 at 1.212 kb on + strand at 1.363 kb on + strand at 1.363 kb on + strand at 1.363 kb on + strand at 1.479 kb on + strand at 1.621 kb on - strand, within GFF4671 at 1.633 kb on - strand, within GFF4671 at 1.788 kb on + strand, within GFF4671 at 1.813 kb on - strand, within GFF4671 at 1.855 kb on - strand, within GFF4671 at 1.904 kb on + strand, within GFF4671 at 2.154 kb on + strand, within GFF4671 at 2.182 kb on - strand, within GFF4671 at 2.269 kb on + strand, within GFF4671 at 2.451 kb on - strand, within GFF4671 at 2.494 kb on + strand, within GFF4671 at 2.834 kb on - strand, within GFF4671 at 2.893 kb on + strand, within GFF4671 at 2.893 kb on + strand, within GFF4671 at 2.900 kb on + strand, within GFF4671 at 2.965 kb on + strand, within GFF4671 at 2.966 kb on - strand, within GFF4671 at 3.016 kb on + strand, within GFF4671 at 3.091 kb on + strand, within GFF4671 at 3.333 kb on + strand, within GFF4672 at 3.390 kb on - strand, within GFF4672 at 3.585 kb on + strand at 3.707 kb on - strand, within GFF4673 at 3.888 kb on - strand, within GFF4673 at 3.890 kb on - strand, within GFF4673 at 3.890 kb on - strand, within GFF4673 at 3.890 kb on - strand, within GFF4673 at 4.064 kb on - strand, within GFF4673 at 4.082 kb on + strand, within GFF4673 at 4.082 kb on + strand, within GFF4673 at 4.278 kb on - strand, within GFF4673
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 458 + +0.6 660 - -0.7 709 + -1.1 800 + GFF4670 0.22 -0.0 804 - GFF4670 0.23 -0.5 935 + GFF4670 0.48 +0.7 1,137 + GFF4670 0.85 -0.8 1,138 - GFF4670 0.86 -0.7 1,212 + -0.9 1,363 + +0.2 1,363 + -1.2 1,363 + -1.1 1,479 + +0.0 1,621 - GFF4671 0.17 -0.0 1,633 - GFF4671 0.18 +1.2 1,788 + GFF4671 0.25 -0.3 1,813 - GFF4671 0.26 +1.1 1,855 - GFF4671 0.28 +0.3 1,904 + GFF4671 0.31 -1.0 2,154 + GFF4671 0.43 -1.7 2,182 - GFF4671 0.45 -0.3 2,269 + GFF4671 0.49 -0.7 2,451 - GFF4671 0.58 -0.2 2,494 + GFF4671 0.60 -0.1 2,834 - GFF4671 0.77 +0.8 2,893 + GFF4671 0.79 -1.0 2,893 + GFF4671 0.79 -0.7 2,900 + GFF4671 0.80 -0.6 2,965 + GFF4671 0.83 -0.6 2,966 - GFF4671 0.83 -0.5 3,016 + GFF4671 0.86 -0.2 3,091 + GFF4671 0.89 -0.4 3,333 + GFF4672 0.13 -0.3 3,390 - GFF4672 0.41 +0.5 3,585 + -0.4 3,707 - GFF4673 0.13 -0.1 3,888 - GFF4673 0.24 +0.7 3,890 - GFF4673 0.25 +0.3 3,890 - GFF4673 0.25 +0.6 3,890 - GFF4673 0.25 -0.2 4,064 - GFF4673 0.36 +0.3 4,082 + GFF4673 0.37 -0.6 4,082 + GFF4673 0.37 +0.1 4,278 - GFF4673 0.50 -1.8
Or see this region's nucleotide sequence