Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4643

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4641 and GFF4642 overlap by 1 nucleotidesGFF4642 and GFF4643 are separated by 28 nucleotidesGFF4643 and GFF4644 are separated by 86 nucleotidesGFF4644 and GFF4645 are separated by 621 nucleotides GFF4641 - Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5), at 436,455 to 437,621 GFF4641 GFF4642 - Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5), at 437,621 to 438,490 GFF4642 GFF4643 - hypothetical protein, at 438,519 to 438,644 GFF4643 GFF4644 - hypothetical protein, at 438,731 to 438,856 GFF4644 GFF4645 - hypothetical protein, at 439,478 to 439,603 GFF4645 Position (kb) 438 439Strain fitness (log2 ratio) -1 0 1 2at 437.620 kb on - strandat 437.636 kb on - strandat 437.645 kb on - strandat 438.095 kb on + strand, within GFF4642at 438.096 kb on - strand, within GFF4642at 438.572 kb on + strand, within GFF4643at 438.807 kb on - strand, within GFF4644at 438.850 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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437,620 - -0.2
437,636 - +0.1
437,645 - -0.8
438,095 + GFF4642 0.54 +0.7
438,096 - GFF4642 0.55 -1.0
438,572 + GFF4643 0.42 -0.8
438,807 - GFF4644 0.60 -1.2
438,850 - +1.8

Or see this region's nucleotide sequence