Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4549 and GFF4550 are separated by 3 nucleotides GFF4550 and GFF4551 are separated by 328 nucleotides GFF4551 and GFF4552 are separated by 96 nucleotides
GFF4549 - LSU m3Psi1915 methyltransferase RlmH, at 334,169 to 334,636
GFF4549
GFF4550 - Iojap protein, at 334,640 to 334,957
GFF4550
GFF4551 - 'Alpha-ribazole-5'-phosphate phosphatase (EC 3.1.3.73)' transl_table=11, at 335,286 to 335,894
GFF4551
GFF4552 - L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), at 335,991 to 337,085
GFF4552
Position (kb) 335
336 Strain fitness (log2 ratio) -2
-1
0
1 at 334.386 kb on - strand, within GFF4549 at 334.570 kb on - strand, within GFF4549 at 334.808 kb on - strand, within GFF4550 at 334.814 kb on - strand, within GFF4550 at 334.850 kb on - strand, within GFF4550 at 334.884 kb on - strand, within GFF4550 at 334.884 kb on - strand, within GFF4550 at 335.066 kb on - strand at 335.069 kb on - strand at 335.099 kb on - strand at 335.312 kb on + strand at 335.316 kb on + strand at 335.346 kb on + strand at 335.346 kb on + strand at 335.347 kb on - strand, within GFF4551 at 335.347 kb on - strand, within GFF4551 at 335.347 kb on - strand, within GFF4551 at 335.480 kb on + strand, within GFF4551 at 335.647 kb on + strand, within GFF4551 at 335.648 kb on - strand, within GFF4551 at 335.658 kb on - strand, within GFF4551 at 335.777 kb on + strand, within GFF4551 at 335.778 kb on - strand, within GFF4551 at 335.778 kb on - strand, within GFF4551 at 335.778 kb on - strand, within GFF4551 at 335.778 kb on - strand, within GFF4551 at 335.839 kb on + strand at 335.898 kb on - strand at 335.898 kb on - strand at 335.989 kb on + strand at 336.068 kb on - strand at 336.226 kb on + strand, within GFF4552 at 336.449 kb on + strand, within GFF4552 at 336.563 kb on + strand, within GFF4552 at 336.585 kb on - strand, within GFF4552 at 336.885 kb on + strand, within GFF4552 at 336.885 kb on + strand, within GFF4552
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 334,386 - GFF4549 0.46 +0.8 334,570 - GFF4549 0.86 +1.4 334,808 - GFF4550 0.53 -0.5 334,814 - GFF4550 0.55 -0.2 334,850 - GFF4550 0.66 -0.4 334,884 - GFF4550 0.77 +0.9 334,884 - GFF4550 0.77 +1.7 335,066 - -0.3 335,069 - -1.1 335,099 - -1.2 335,312 + +0.3 335,316 + -0.5 335,346 + +0.5 335,346 + -0.4 335,347 - GFF4551 0.10 -0.3 335,347 - GFF4551 0.10 -0.4 335,347 - GFF4551 0.10 -0.3 335,480 + GFF4551 0.32 -0.0 335,647 + GFF4551 0.59 -0.5 335,648 - GFF4551 0.59 -0.2 335,658 - GFF4551 0.61 -0.0 335,777 + GFF4551 0.81 +0.4 335,778 - GFF4551 0.81 +0.2 335,778 - GFF4551 0.81 +0.9 335,778 - GFF4551 0.81 -0.6 335,778 - GFF4551 0.81 -2.2 335,839 + +0.7 335,898 - -1.3 335,898 - -0.3 335,989 + -0.2 336,068 - -0.4 336,226 + GFF4552 0.21 -0.2 336,449 + GFF4552 0.42 +0.4 336,563 + GFF4552 0.52 +0.1 336,585 - GFF4552 0.54 -1.1 336,885 + GFF4552 0.82 -0.9 336,885 + GFF4552 0.82 -0.3
Or see this region's nucleotide sequence