Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4515 and GFF4516 are separated by 223 nucleotides GFF4516 and GFF4517 overlap by 4 nucleotides GFF4517 and GFF4518 overlap by 1 nucleotides
GFF4515 - Putative oxidoreductase component of anaerobic dehydrogenases; Chaperone protein TorD, at 303,365 to 303,925
GFF4515
GFF4516 - Putative oxidoreductase STM0611-0613, chain A, at 304,149 to 306,197
GFF4516
GFF4517 - Anaerobic dimethyl sulfoxide reductase chain B (EC 1.8.5.3), at 306,194 to 306,751
GFF4517
GFF4518 - Anaerobic dimethyl sulfoxide reductase chain C (EC 1.8.5.3), at 306,751 to 307,518
GFF4518
Position (kb) 304
305
306
307 Strain fitness (log2 ratio) -1
0
1 at 303.880 kb on - strand at 304.077 kb on - strand at 304.143 kb on - strand at 304.176 kb on - strand at 304.241 kb on + strand at 304.242 kb on - strand at 304.359 kb on - strand, within GFF4516 at 304.383 kb on + strand, within GFF4516 at 304.411 kb on - strand, within GFF4516 at 304.411 kb on - strand, within GFF4516 at 304.444 kb on - strand, within GFF4516 at 304.446 kb on - strand, within GFF4516 at 304.501 kb on - strand, within GFF4516 at 304.539 kb on - strand, within GFF4516 at 304.562 kb on + strand, within GFF4516 at 304.608 kb on + strand, within GFF4516 at 304.692 kb on + strand, within GFF4516 at 304.758 kb on - strand, within GFF4516 at 304.821 kb on + strand, within GFF4516 at 305.057 kb on + strand, within GFF4516 at 305.073 kb on + strand, within GFF4516 at 305.073 kb on + strand, within GFF4516 at 305.075 kb on + strand, within GFF4516 at 305.268 kb on - strand, within GFF4516 at 305.482 kb on - strand, within GFF4516 at 305.535 kb on - strand, within GFF4516 at 305.535 kb on - strand, within GFF4516 at 305.731 kb on - strand, within GFF4516 at 305.908 kb on - strand, within GFF4516 at 306.040 kb on - strand at 306.040 kb on - strand at 306.066 kb on + strand at 306.141 kb on - strand at 306.223 kb on + strand at 306.326 kb on + strand, within GFF4517 at 306.326 kb on + strand, within GFF4517 at 306.327 kb on - strand, within GFF4517 at 306.327 kb on - strand, within GFF4517 at 306.445 kb on + strand, within GFF4517 at 306.485 kb on - strand, within GFF4517 at 306.511 kb on + strand, within GFF4517 at 306.512 kb on - strand, within GFF4517 at 306.544 kb on + strand, within GFF4517 at 306.544 kb on + strand, within GFF4517 at 306.682 kb on + strand, within GFF4517 at 306.712 kb on + strand at 306.791 kb on + strand at 306.868 kb on + strand, within GFF4518 at 306.868 kb on + strand, within GFF4518 at 306.906 kb on + strand, within GFF4518 at 306.946 kb on + strand, within GFF4518 at 306.947 kb on - strand, within GFF4518 at 306.947 kb on - strand, within GFF4518 at 306.980 kb on + strand, within GFF4518
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 303,880 - +0.1 304,077 - -1.4 304,143 - +0.3 304,176 - +0.6 304,241 + -0.5 304,242 - +0.9 304,359 - GFF4516 0.10 -0.1 304,383 + GFF4516 0.11 -0.4 304,411 - GFF4516 0.13 -0.3 304,411 - GFF4516 0.13 -0.8 304,444 - GFF4516 0.14 -0.0 304,446 - GFF4516 0.14 -0.8 304,501 - GFF4516 0.17 -0.0 304,539 - GFF4516 0.19 +1.3 304,562 + GFF4516 0.20 +0.6 304,608 + GFF4516 0.22 -0.6 304,692 + GFF4516 0.27 +0.3 304,758 - GFF4516 0.30 +0.3 304,821 + GFF4516 0.33 +0.5 305,057 + GFF4516 0.44 -0.6 305,073 + GFF4516 0.45 +0.4 305,073 + GFF4516 0.45 +0.2 305,075 + GFF4516 0.45 +0.1 305,268 - GFF4516 0.55 -0.2 305,482 - GFF4516 0.65 -0.9 305,535 - GFF4516 0.68 -0.1 305,535 - GFF4516 0.68 +0.5 305,731 - GFF4516 0.77 +0.1 305,908 - GFF4516 0.86 +0.6 306,040 - +0.0 306,040 - -0.6 306,066 + -0.0 306,141 - +0.3 306,223 + +0.1 306,326 + GFF4517 0.24 +1.0 306,326 + GFF4517 0.24 +0.1 306,327 - GFF4517 0.24 +0.0 306,327 - GFF4517 0.24 -0.2 306,445 + GFF4517 0.45 -0.2 306,485 - GFF4517 0.52 +0.8 306,511 + GFF4517 0.57 -1.2 306,512 - GFF4517 0.57 -0.7 306,544 + GFF4517 0.63 +0.4 306,544 + GFF4517 0.63 +0.3 306,682 + GFF4517 0.87 +0.1 306,712 + +0.7 306,791 + -1.1 306,868 + GFF4518 0.15 -0.1 306,868 + GFF4518 0.15 +0.4 306,906 + GFF4518 0.20 -0.0 306,946 + GFF4518 0.25 -0.5 306,947 - GFF4518 0.26 +0.1 306,947 - GFF4518 0.26 -0.1 306,980 + GFF4518 0.30 +0.1
Or see this region's nucleotide sequence