Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4369 and GFF4370 are separated by 214 nucleotides GFF4370 and GFF4371 are separated by 32 nucleotides GFF4371 and GFF4372 are separated by 230 nucleotides
GFF4369 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 152,494 to 153,354
GFF4369
GFF4370 - Glycoprotein-polysaccharide metabolism, at 153,569 to 154,138
GFF4370
GFF4371 - methylated-DNA--protein-cysteine methyltransferase-related protein, at 154,171 to 154,560
GFF4371
GFF4372 - Uncharacterized protein ylaB, at 154,791 to 156,341
GFF4372
Position (kb) 154
155 Strain fitness (log2 ratio) -1
0
1
2 at 153.354 kb on + strand at 153.354 kb on + strand at 153.355 kb on - strand at 153.358 kb on + strand at 153.401 kb on + strand at 153.402 kb on - strand at 153.685 kb on + strand, within GFF4370 at 153.685 kb on + strand, within GFF4370 at 153.698 kb on - strand, within GFF4370 at 153.835 kb on - strand, within GFF4370 at 153.893 kb on - strand, within GFF4370 at 153.956 kb on - strand, within GFF4370 at 153.997 kb on + strand, within GFF4370 at 154.172 kb on + strand at 154.275 kb on + strand, within GFF4371 at 154.486 kb on - strand, within GFF4371 at 154.817 kb on + strand at 154.818 kb on - strand at 154.846 kb on + strand at 154.848 kb on + strand at 154.848 kb on + strand at 154.869 kb on + strand at 154.931 kb on + strand at 154.980 kb on - strand, within GFF4372 at 155.060 kb on - strand, within GFF4372 at 155.214 kb on + strand, within GFF4372 at 155.270 kb on + strand, within GFF4372 at 155.427 kb on + strand, within GFF4372 at 155.471 kb on + strand, within GFF4372 at 155.471 kb on + strand, within GFF4372 at 155.484 kb on + strand, within GFF4372 at 155.484 kb on + strand, within GFF4372 at 155.484 kb on + strand, within GFF4372 at 155.484 kb on + strand, within GFF4372 at 155.484 kb on + strand, within GFF4372 at 155.553 kb on - strand, within GFF4372
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 153,354 + +0.2 153,354 + -0.2 153,355 - +0.4 153,358 + +0.3 153,401 + -1.2 153,402 - -1.1 153,685 + GFF4370 0.20 +0.5 153,685 + GFF4370 0.20 -0.8 153,698 - GFF4370 0.23 -0.7 153,835 - GFF4370 0.47 +0.4 153,893 - GFF4370 0.57 -0.0 153,956 - GFF4370 0.68 -0.5 153,997 + GFF4370 0.75 +0.7 154,172 + +0.3 154,275 + GFF4371 0.27 -1.1 154,486 - GFF4371 0.81 -0.1 154,817 + +1.0 154,818 - +1.9 154,846 + +0.6 154,848 + -0.7 154,848 + -0.2 154,869 + -0.0 154,931 + -0.2 154,980 - GFF4372 0.12 -0.6 155,060 - GFF4372 0.17 -0.4 155,214 + GFF4372 0.27 -0.5 155,270 + GFF4372 0.31 -1.6 155,427 + GFF4372 0.41 -0.3 155,471 + GFF4372 0.44 -0.0 155,471 + GFF4372 0.44 -0.5 155,484 + GFF4372 0.45 +0.7 155,484 + GFF4372 0.45 +0.4 155,484 + GFF4372 0.45 +0.7 155,484 + GFF4372 0.45 -0.5 155,484 + GFF4372 0.45 +0.4 155,553 - GFF4372 0.49 +2.3
Or see this region's nucleotide sequence