Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4361 and GFF4362 are separated by 100 nucleotides GFF4362 and GFF4363 are separated by 49 nucleotides
GFF4361 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 142,643 to 144,343
GFF4361
GFF4362 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 144,444 to 145,262
GFF4362
GFF4363 - Cysteine synthase B (EC 2.5.1.47), at 145,312 to 146,367
GFF4363
Position (kb)
144
145
146 Strain fitness (log2 ratio)
-1
0
1 at 143.484 kb on + strand, within GFF4361 at 143.589 kb on + strand, within GFF4361 at 143.590 kb on - strand, within GFF4361 at 143.590 kb on - strand, within GFF4361 at 143.710 kb on + strand, within GFF4361 at 143.764 kb on + strand, within GFF4361 at 143.962 kb on - strand, within GFF4361 at 143.986 kb on + strand, within GFF4361 at 144.066 kb on - strand, within GFF4361 at 144.335 kb on + strand at 144.381 kb on - strand at 144.468 kb on - strand at 144.481 kb on + strand at 144.484 kb on + strand at 144.485 kb on - strand at 144.625 kb on - strand, within GFF4362 at 145.243 kb on + strand at 145.243 kb on + strand at 145.244 kb on - strand at 145.244 kb on - strand at 145.249 kb on - strand at 145.394 kb on + strand at 145.398 kb on - strand at 145.398 kb on - strand at 145.398 kb on - strand at 145.398 kb on - strand at 145.398 kb on - strand at 145.500 kb on + strand, within GFF4363 at 145.746 kb on + strand, within GFF4363 at 145.751 kb on + strand, within GFF4363 at 145.752 kb on - strand, within GFF4363 at 145.767 kb on + strand, within GFF4363 at 145.768 kb on - strand, within GFF4363 at 145.931 kb on + strand, within GFF4363 at 145.931 kb on + strand, within GFF4363 at 145.931 kb on + strand, within GFF4363 at 145.932 kb on - strand, within GFF4363 at 146.180 kb on + strand, within GFF4363 at 146.250 kb on - strand, within GFF4363
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 143,484 + GFF4361 0.49 +0.1 143,589 + GFF4361 0.56 +0.6 143,590 - GFF4361 0.56 -0.2 143,590 - GFF4361 0.56 -0.7 143,710 + GFF4361 0.63 +0.5 143,764 + GFF4361 0.66 -0.0 143,962 - GFF4361 0.78 -0.7 143,986 + GFF4361 0.79 +0.0 144,066 - GFF4361 0.84 +1.3 144,335 + +0.2 144,381 - -0.4 144,468 - -0.1 144,481 + -0.3 144,484 + -0.4 144,485 - +0.7 144,625 - GFF4362 0.22 +0.4 145,243 + +0.3 145,243 + -0.7 145,244 - +1.7 145,244 - +0.2 145,249 - -0.0 145,394 + +1.1 145,398 - -1.1 145,398 - -0.2 145,398 - -0.5 145,398 - +0.4 145,398 - +0.3 145,500 + GFF4363 0.18 +0.1 145,746 + GFF4363 0.41 +0.6 145,751 + GFF4363 0.42 +0.8 145,752 - GFF4363 0.42 -0.3 145,767 + GFF4363 0.43 +1.3 145,768 - GFF4363 0.43 +1.2 145,931 + GFF4363 0.59 +1.2 145,931 + GFF4363 0.59 -0.7 145,931 + GFF4363 0.59 +1.4 145,932 - GFF4363 0.59 -0.0 146,180 + GFF4363 0.82 -0.1 146,250 - GFF4363 0.89 -0.3
Or see this region's nucleotide sequence