Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4273 and GFF4274 are separated by 37 nucleotides GFF4274 and GFF4275 are separated by 267 nucleotides GFF4275 and GFF4276 are separated by 25 nucleotides GFF4276 and GFF4277 are separated by 107 nucleotides GFF4277 and GFF4278 are separated by 168 nucleotides
GFF4273 - Putative outer membrane lipoprotein, at 61,806 to 62,900
GFF4273
GFF4274 - Putative inner membrane protein, at 62,938 to 63,246
GFF4274
GFF4275 - Putative inner membrane protein, at 63,514 to 63,729
GFF4275
GFF4276 - D-alanine--D-alanine ligase A (EC 6.3.2.4), at 63,755 to 64,849
GFF4276
GFF4277 - FIG01045207: hypothetical protein, at 64,957 to 65,640
GFF4277
GFF4278 - Multidrug-efflux transporter, major facilitator superfamily (MFS) (TC 2.A.1), at 65,809 to 67,020
GFF4278
Position (kb) 63
64
65 Strain fitness (log2 ratio) -2
-1
0
1
2 at 62.888 kb on + strand at 63.092 kb on + strand, within GFF4274 at 63.093 kb on - strand, within GFF4274 at 63.154 kb on + strand, within GFF4274 at 63.155 kb on - strand, within GFF4274 at 63.499 kb on + strand at 63.500 kb on - strand at 63.514 kb on - strand at 63.550 kb on + strand, within GFF4275 at 63.553 kb on + strand, within GFF4275 at 63.553 kb on + strand, within GFF4275 at 63.597 kb on - strand, within GFF4275 at 63.660 kb on + strand, within GFF4275 at 63.868 kb on - strand, within GFF4276 at 63.872 kb on - strand, within GFF4276 at 64.070 kb on - strand, within GFF4276 at 64.071 kb on + strand, within GFF4276 at 64.071 kb on + strand, within GFF4276 at 64.071 kb on + strand, within GFF4276 at 64.071 kb on + strand, within GFF4276 at 64.072 kb on - strand, within GFF4276 at 64.072 kb on - strand, within GFF4276 at 64.072 kb on - strand, within GFF4276 at 64.072 kb on - strand, within GFF4276 at 64.075 kb on + strand, within GFF4276 at 64.148 kb on + strand, within GFF4276 at 64.148 kb on + strand, within GFF4276 at 64.148 kb on + strand, within GFF4276 at 64.149 kb on - strand, within GFF4276 at 64.149 kb on - strand, within GFF4276 at 64.149 kb on - strand, within GFF4276 at 64.261 kb on + strand, within GFF4276 at 64.372 kb on + strand, within GFF4276 at 64.372 kb on + strand, within GFF4276 at 64.476 kb on - strand, within GFF4276 at 64.546 kb on + strand, within GFF4276 at 64.546 kb on + strand, within GFF4276 at 64.652 kb on - strand, within GFF4276 at 64.652 kb on - strand, within GFF4276 at 64.652 kb on - strand, within GFF4276 at 64.718 kb on - strand, within GFF4276 at 64.722 kb on - strand, within GFF4276 at 64.824 kb on - strand at 64.824 kb on - strand at 64.824 kb on - strand at 64.827 kb on - strand at 64.888 kb on + strand at 65.021 kb on - strand at 65.032 kb on + strand, within GFF4277 at 65.085 kb on + strand, within GFF4277 at 65.096 kb on + strand, within GFF4277 at 65.097 kb on - strand, within GFF4277 at 65.201 kb on + strand, within GFF4277 at 65.371 kb on + strand, within GFF4277 at 65.372 kb on - strand, within GFF4277 at 65.534 kb on + strand, within GFF4277 at 65.578 kb on + strand at 65.580 kb on + strand at 65.646 kb on - strand at 65.670 kb on + strand at 65.670 kb on + strand at 65.793 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 62,888 + +2.1 63,092 + GFF4274 0.50 +0.3 63,093 - GFF4274 0.50 -0.1 63,154 + GFF4274 0.70 +1.0 63,155 - GFF4274 0.70 +0.3 63,499 + -0.3 63,500 - -0.3 63,514 - -0.8 63,550 + GFF4275 0.17 -0.8 63,553 + GFF4275 0.18 -0.4 63,553 + GFF4275 0.18 -0.8 63,597 - GFF4275 0.38 -0.9 63,660 + GFF4275 0.68 -0.3 63,868 - GFF4276 0.10 -0.4 63,872 - GFF4276 0.11 +0.0 64,070 - GFF4276 0.29 -0.1 64,071 + GFF4276 0.29 -0.9 64,071 + GFF4276 0.29 -0.0 64,071 + GFF4276 0.29 -1.0 64,071 + GFF4276 0.29 -0.2 64,072 - GFF4276 0.29 -0.1 64,072 - GFF4276 0.29 +0.5 64,072 - GFF4276 0.29 -0.0 64,072 - GFF4276 0.29 +0.1 64,075 + GFF4276 0.29 +0.1 64,148 + GFF4276 0.36 +0.5 64,148 + GFF4276 0.36 -0.9 64,148 + GFF4276 0.36 +0.5 64,149 - GFF4276 0.36 -0.1 64,149 - GFF4276 0.36 -1.0 64,149 - GFF4276 0.36 -0.0 64,261 + GFF4276 0.46 -0.1 64,372 + GFF4276 0.56 +1.6 64,372 + GFF4276 0.56 +0.2 64,476 - GFF4276 0.66 -0.6 64,546 + GFF4276 0.72 +0.8 64,546 + GFF4276 0.72 +0.7 64,652 - GFF4276 0.82 +0.5 64,652 - GFF4276 0.82 -1.3 64,652 - GFF4276 0.82 -0.1 64,718 - GFF4276 0.88 +0.4 64,722 - GFF4276 0.88 -0.3 64,824 - -0.5 64,824 - +0.2 64,824 - -0.3 64,827 - -0.6 64,888 + -0.4 65,021 - +0.0 65,032 + GFF4277 0.11 +2.1 65,085 + GFF4277 0.19 -2.0 65,096 + GFF4277 0.20 +0.3 65,097 - GFF4277 0.20 -0.7 65,201 + GFF4277 0.36 -0.0 65,371 + GFF4277 0.61 +0.0 65,372 - GFF4277 0.61 +0.2 65,534 + GFF4277 0.84 +0.3 65,578 + +0.8 65,580 + +0.6 65,646 - -0.4 65,670 + -0.1 65,670 + +0.2 65,793 + +0.1
Or see this region's nucleotide sequence