Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4154 and GFF4155 overlap by 4 nucleotides GFF4155 and GFF4156 are separated by 64 nucleotides
GFF4154 - Type I secretion system ATPase, LssB family LapB, at 34,792 to 36,972
GFF4154
GFF4155 - Type I secretion system, outer membrane component LapE, at 36,969 to 38,378
GFF4155
GFF4156 - T1SS secreted agglutinin RTX, at 38,443 to 49,917
GFF4156
Position (kb) 36
37
38
39 Strain fitness (log2 ratio) -1
0
1
2 at 36.087 kb on + strand, within GFF4154 at 36.088 kb on - strand, within GFF4154 at 36.138 kb on + strand, within GFF4154 at 36.139 kb on - strand, within GFF4154 at 36.205 kb on - strand, within GFF4154 at 36.364 kb on - strand, within GFF4154 at 36.369 kb on - strand, within GFF4154 at 36.466 kb on + strand, within GFF4154 at 36.470 kb on + strand, within GFF4154 at 36.471 kb on - strand, within GFF4154 at 36.471 kb on - strand, within GFF4154 at 36.528 kb on + strand, within GFF4154 at 36.529 kb on - strand, within GFF4154 at 36.585 kb on - strand, within GFF4154 at 36.676 kb on - strand, within GFF4154 at 36.742 kb on - strand, within GFF4154 at 36.832 kb on - strand at 36.896 kb on + strand at 36.950 kb on - strand at 37.040 kb on + strand at 37.042 kb on + strand at 37.042 kb on + strand at 37.108 kb on + strand at 37.177 kb on + strand, within GFF4155 at 37.405 kb on + strand, within GFF4155 at 37.439 kb on - strand, within GFF4155 at 37.442 kb on + strand, within GFF4155 at 37.462 kb on - strand, within GFF4155 at 37.508 kb on - strand, within GFF4155 at 37.799 kb on + strand, within GFF4155 at 37.843 kb on - strand, within GFF4155 at 37.869 kb on + strand, within GFF4155 at 37.870 kb on - strand, within GFF4155 at 37.870 kb on - strand, within GFF4155 at 38.014 kb on - strand, within GFF4155 at 38.041 kb on + strand, within GFF4155 at 38.042 kb on - strand, within GFF4155 at 38.054 kb on - strand, within GFF4155 at 38.090 kb on - strand, within GFF4155 at 38.277 kb on + strand at 38.278 kb on - strand at 38.291 kb on + strand at 38.292 kb on - strand at 38.383 kb on + strand at 38.485 kb on + strand at 38.530 kb on - strand at 38.563 kb on + strand at 38.571 kb on + strand at 38.867 kb on + strand at 38.871 kb on + strand at 38.872 kb on - strand at 38.872 kb on - strand at 38.872 kb on - strand at 38.931 kb on - strand at 39.375 kb on + strand at 39.375 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 36,087 + GFF4154 0.59 +0.4 36,088 - GFF4154 0.59 +0.3 36,138 + GFF4154 0.62 +0.8 36,139 - GFF4154 0.62 +0.5 36,205 - GFF4154 0.65 +0.6 36,364 - GFF4154 0.72 -0.0 36,369 - GFF4154 0.72 +0.4 36,466 + GFF4154 0.77 +0.6 36,470 + GFF4154 0.77 +0.2 36,471 - GFF4154 0.77 +0.8 36,471 - GFF4154 0.77 -0.1 36,528 + GFF4154 0.80 +0.6 36,529 - GFF4154 0.80 +0.3 36,585 - GFF4154 0.82 +1.6 36,676 - GFF4154 0.86 -0.4 36,742 - GFF4154 0.89 -0.2 36,832 - -0.2 36,896 + -0.0 36,950 - +0.9 37,040 + +0.7 37,042 + -0.0 37,042 + +0.4 37,108 + +0.4 37,177 + GFF4155 0.15 +0.3 37,405 + GFF4155 0.31 +1.2 37,439 - GFF4155 0.33 -0.4 37,442 + GFF4155 0.34 -0.7 37,462 - GFF4155 0.35 +0.5 37,508 - GFF4155 0.38 +0.4 37,799 + GFF4155 0.59 +0.2 37,843 - GFF4155 0.62 -0.1 37,869 + GFF4155 0.64 -1.3 37,870 - GFF4155 0.64 -0.3 37,870 - GFF4155 0.64 +0.4 38,014 - GFF4155 0.74 -0.2 38,041 + GFF4155 0.76 +0.4 38,042 - GFF4155 0.76 +0.2 38,054 - GFF4155 0.77 +0.6 38,090 - GFF4155 0.80 +1.2 38,277 + +0.2 38,278 - +0.6 38,291 + -0.5 38,292 - +0.5 38,383 + -0.7 38,485 + +0.8 38,530 - -0.7 38,563 + +0.1 38,571 + +0.2 38,867 + +0.2 38,871 + +1.0 38,872 - +0.9 38,872 - +0.2 38,872 - +0.2 38,931 - -1.3 39,375 + +0.0 39,375 + +2.3
Or see this region's nucleotide sequence