Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4006 and GFF4007 are separated by 8 nucleotides GFF4007 and GFF4008 are separated by 9 nucleotides GFF4008 and GFF4009 overlap by 1 nucleotides GFF4009 and GFF4010 are separated by 56 nucleotides GFF4010 and GFF4011 overlap by 4 nucleotides
GFF4006 - Flagellar biosynthesis protein FliR, at 277,708 to 278,502
GFF4006
GFF4007 - Flagellar biosynthesis protein FliQ, at 278,511 to 278,780
GFF4007
GFF4008 - Flagellar biosynthesis protein FliP, at 278,790 to 279,527
GFF4008
GFF4009 - Flagellar biosynthesis protein FliQ, at 279,527 to 279,847
GFF4009
GFF4010 - Flagellar motor switch protein FliN, at 279,904 to 280,317
GFF4010
GFF4011 - Flagellar motor switch protein FliM, at 280,314 to 281,318
GFF4011
Position (kb) 278
279
280 Strain fitness (log2 ratio) -1
0
1
2
3 at 278.099 kb on - strand, within GFF4006 at 278.113 kb on - strand, within GFF4006 at 278.118 kb on + strand, within GFF4006 at 278.139 kb on + strand, within GFF4006 at 278.457 kb on - strand at 278.472 kb on - strand at 278.473 kb on + strand at 278.522 kb on + strand at 278.525 kb on - strand at 278.554 kb on + strand, within GFF4007 at 278.594 kb on + strand, within GFF4007 at 278.707 kb on - strand, within GFF4007 at 279.262 kb on - strand, within GFF4008 at 279.513 kb on + strand at 279.513 kb on + strand at 279.817 kb on + strand at 279.828 kb on - strand at 279.925 kb on - strand at 279.942 kb on + strand at 279.969 kb on + strand, within GFF4010 at 280.167 kb on + strand, within GFF4010 at 280.168 kb on - strand, within GFF4010 at 280.303 kb on - strand at 280.368 kb on + strand at 280.381 kb on + strand at 280.443 kb on - strand, within GFF4011 at 280.443 kb on - strand, within GFF4011
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 278,099 - GFF4006 0.49 +0.2 278,113 - GFF4006 0.51 +0.7 278,118 + GFF4006 0.52 +1.1 278,139 + GFF4006 0.54 +2.2 278,457 - -1.3 278,472 - +1.8 278,473 + +1.5 278,522 + +1.0 278,525 - +1.3 278,554 + GFF4007 0.16 -0.7 278,594 + GFF4007 0.31 +2.0 278,707 - GFF4007 0.73 +0.9 279,262 - GFF4008 0.64 +1.0 279,513 + +1.4 279,513 + +2.1 279,817 + +1.8 279,828 - +0.8 279,925 - +0.6 279,942 + +2.2 279,969 + GFF4010 0.16 +1.3 280,167 + GFF4010 0.64 +0.2 280,168 - GFF4010 0.64 +0.9 280,303 - +0.8 280,368 + +1.1 280,381 + +0.9 280,443 - GFF4011 0.13 +0.8 280,443 - GFF4011 0.13 +2.9
Or see this region's nucleotide sequence