Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3923 and GFF3924 are separated by 152 nucleotides GFF3924 and GFF3925 are separated by 118 nucleotides GFF3925 and GFF3926 are separated by 244 nucleotides GFF3926 and GFF3927 are separated by 33 nucleotides
GFF3923 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 207,656 to 208,828
GFF3923
GFF3924 - DNA gyrase inhibitory protein, at 208,981 to 209,448
GFF3924
GFF3925 - Putative inner membrane protein, at 209,567 to 210,625
GFF3925
GFF3926 - UPF0265 protein YeeX, at 210,870 to 211,205
GFF3926
GFF3927 - Threonine kinase in B12 biosynthesis, at 211,239 to 212,105
GFF3927
Position (kb) 209
210
211 Strain fitness (log2 ratio) -1
0
1
2 at 208.652 kb on + strand, within GFF3923 at 208.664 kb on - strand, within GFF3923 at 208.786 kb on + strand at 208.827 kb on - strand at 209.018 kb on + strand at 209.153 kb on + strand, within GFF3924 at 209.153 kb on + strand, within GFF3924 at 209.348 kb on - strand, within GFF3924 at 209.354 kb on + strand, within GFF3924 at 209.427 kb on + strand at 209.519 kb on + strand at 209.520 kb on - strand at 209.529 kb on - strand at 209.529 kb on - strand at 209.586 kb on + strand at 209.609 kb on + strand at 209.610 kb on - strand at 209.642 kb on - strand at 209.722 kb on - strand, within GFF3925 at 209.731 kb on + strand, within GFF3925 at 209.934 kb on + strand, within GFF3925 at 210.119 kb on + strand, within GFF3925 at 210.119 kb on + strand, within GFF3925 at 210.295 kb on + strand, within GFF3925 at 210.295 kb on + strand, within GFF3925 at 210.296 kb on - strand, within GFF3925 at 210.475 kb on + strand, within GFF3925 at 210.476 kb on - strand, within GFF3925 at 210.558 kb on + strand at 210.693 kb on - strand at 210.712 kb on + strand at 210.815 kb on + strand at 211.104 kb on + strand, within GFF3926 at 211.104 kb on + strand, within GFF3926 at 211.104 kb on + strand, within GFF3926 at 211.112 kb on + strand, within GFF3926 at 211.193 kb on - strand at 211.212 kb on - strand at 211.227 kb on + strand at 211.323 kb on - strand at 211.340 kb on + strand, within GFF3927 at 211.386 kb on - strand, within GFF3927 at 211.406 kb on - strand, within GFF3927 at 211.459 kb on - strand, within GFF3927 at 211.527 kb on - strand, within GFF3927
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 208,652 + GFF3923 0.85 -1.2 208,664 - GFF3923 0.86 -0.2 208,786 + +0.5 208,827 - -0.1 209,018 + -0.3 209,153 + GFF3924 0.37 -0.7 209,153 + GFF3924 0.37 -0.2 209,348 - GFF3924 0.78 +0.2 209,354 + GFF3924 0.80 +0.1 209,427 + -0.3 209,519 + +2.2 209,520 - -0.4 209,529 - -1.4 209,529 - -0.1 209,586 + -0.1 209,609 + -0.9 209,610 - -0.4 209,642 - -0.4 209,722 - GFF3925 0.15 -1.1 209,731 + GFF3925 0.15 -0.2 209,934 + GFF3925 0.35 -0.8 210,119 + GFF3925 0.52 -0.5 210,119 + GFF3925 0.52 +0.2 210,295 + GFF3925 0.69 +0.2 210,295 + GFF3925 0.69 -0.4 210,296 - GFF3925 0.69 -0.2 210,475 + GFF3925 0.86 -0.1 210,476 - GFF3925 0.86 -0.6 210,558 + -0.9 210,693 - -0.2 210,712 + -0.7 210,815 + -0.8 211,104 + GFF3926 0.70 -0.9 211,104 + GFF3926 0.70 -0.9 211,104 + GFF3926 0.70 -0.9 211,112 + GFF3926 0.72 -0.1 211,193 - -0.1 211,212 - +2.2 211,227 + -0.5 211,323 - -1.1 211,340 + GFF3927 0.12 -0.8 211,386 - GFF3927 0.17 +0.6 211,406 - GFF3927 0.19 -1.3 211,459 - GFF3927 0.25 +0.2 211,527 - GFF3927 0.33 -1.0
Or see this region's nucleotide sequence