Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3733 and GFF3734 are separated by 231 nucleotides GFF3734 and GFF3735 are separated by 317 nucleotides GFF3735 and GFF3736 are separated by 90 nucleotides GFF3736 and GFF3737 are separated by 79 nucleotides
GFF3733 - Phage tail fiber protein, at 140 to 931
GFF3733
GFF3734 - Phage tail fibers, at 1,163 to 1,282
GFF3734
GFF3735 - Secreted effector protein, at 1,600 to 3,966
GFF3735
GFF3736 - Phage-related protein, at 4,057 to 4,287
GFF3736
GFF3737 - Phage antitermination protein Q, at 4,367 to 5,284
GFF3737
Position (kb)
1
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 0.803 kb on + strand, within GFF3733 at 0.804 kb on - strand, within GFF3733 at 0.838 kb on + strand, within GFF3733 at 1.106 kb on - strand at 1.167 kb on + strand at 1.574 kb on - strand at 1.574 kb on - strand at 1.574 kb on - strand at 1.919 kb on + strand, within GFF3735 at 1.919 kb on + strand, within GFF3735 at 1.951 kb on + strand, within GFF3735 at 1.951 kb on + strand, within GFF3735 at 1.951 kb on + strand, within GFF3735 at 1.958 kb on + strand, within GFF3735 at 2.104 kb on + strand, within GFF3735 at 2.295 kb on + strand, within GFF3735 at 2.409 kb on + strand, within GFF3735 at 2.410 kb on - strand, within GFF3735 at 2.451 kb on - strand, within GFF3735 at 2.451 kb on - strand, within GFF3735 at 2.454 kb on + strand, within GFF3735 at 2.454 kb on + strand, within GFF3735 at 2.454 kb on + strand, within GFF3735 at 2.455 kb on - strand, within GFF3735 at 2.455 kb on - strand, within GFF3735 at 2.455 kb on - strand, within GFF3735 at 2.455 kb on - strand, within GFF3735 at 2.575 kb on - strand, within GFF3735 at 2.589 kb on + strand, within GFF3735 at 2.589 kb on + strand, within GFF3735 at 2.590 kb on - strand, within GFF3735 at 2.593 kb on - strand, within GFF3735 at 2.645 kb on + strand, within GFF3735 at 2.690 kb on + strand, within GFF3735 at 2.694 kb on + strand, within GFF3735 at 2.888 kb on - strand, within GFF3735 at 3.153 kb on + strand, within GFF3735 at 3.312 kb on + strand, within GFF3735 at 3.313 kb on - strand, within GFF3735 at 3.380 kb on + strand, within GFF3735 at 3.381 kb on - strand, within GFF3735 at 3.394 kb on - strand, within GFF3735 at 3.689 kb on + strand, within GFF3735 at 3.938 kb on - strand at 4.086 kb on - strand, within GFF3736 at 4.341 kb on + strand at 4.341 kb on + strand at 4.657 kb on + strand, within GFF3737 at 4.933 kb on - strand, within GFF3737
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 803 + GFF3733 0.84 -0.1 804 - GFF3733 0.84 +1.4 838 + GFF3733 0.88 -0.3 1,106 - -0.2 1,167 + -0.6 1,574 - -0.0 1,574 - +0.4 1,574 - -0.4 1,919 + GFF3735 0.13 -0.6 1,919 + GFF3735 0.13 -0.5 1,951 + GFF3735 0.15 -0.1 1,951 + GFF3735 0.15 -0.5 1,951 + GFF3735 0.15 -0.5 1,958 + GFF3735 0.15 -0.6 2,104 + GFF3735 0.21 -0.2 2,295 + GFF3735 0.29 +0.2 2,409 + GFF3735 0.34 -0.8 2,410 - GFF3735 0.34 -0.3 2,451 - GFF3735 0.36 +0.2 2,451 - GFF3735 0.36 -0.5 2,454 + GFF3735 0.36 -0.9 2,454 + GFF3735 0.36 -0.8 2,454 + GFF3735 0.36 +0.8 2,455 - GFF3735 0.36 -1.8 2,455 - GFF3735 0.36 -0.2 2,455 - GFF3735 0.36 +1.8 2,455 - GFF3735 0.36 +0.1 2,575 - GFF3735 0.41 +0.3 2,589 + GFF3735 0.42 -0.5 2,589 + GFF3735 0.42 -0.6 2,590 - GFF3735 0.42 -1.5 2,593 - GFF3735 0.42 +0.6 2,645 + GFF3735 0.44 -0.3 2,690 + GFF3735 0.46 -0.7 2,694 + GFF3735 0.46 +0.3 2,888 - GFF3735 0.54 +0.1 3,153 + GFF3735 0.66 +1.3 3,312 + GFF3735 0.72 +0.6 3,313 - GFF3735 0.72 -0.3 3,380 + GFF3735 0.75 -0.2 3,381 - GFF3735 0.75 -0.4 3,394 - GFF3735 0.76 +0.4 3,689 + GFF3735 0.88 -2.6 3,938 - +0.6 4,086 - GFF3736 0.13 -0.2 4,341 + -0.1 4,341 + -0.5 4,657 + GFF3737 0.32 -0.2 4,933 - GFF3737 0.62 +0.6
Or see this region's nucleotide sequence