Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3661 and GFF3662 are separated by 446 nucleotides GFF3662 and GFF3663 are separated by 136 nucleotides
GFF3661 - Formate efflux transporter (TC 2.A.44 family), at 9,038 to 9,853
GFF3661
GFF3662 - FIG00638589: hypothetical protein, at 10,300 to 12,060
GFF3662
GFF3663 - Putative inner membrane protein, at 12,197 to 12,889
GFF3663
Position (kb)
10
11
12
13 Strain fitness (log2 ratio)
-1
0
1 at 9.349 kb on - strand, within GFF3661 at 9.413 kb on - strand, within GFF3661 at 9.481 kb on + strand, within GFF3661 at 9.482 kb on - strand, within GFF3661 at 9.484 kb on - strand, within GFF3661 at 9.543 kb on + strand, within GFF3661 at 9.593 kb on - strand, within GFF3661 at 9.593 kb on - strand, within GFF3661 at 9.603 kb on - strand, within GFF3661 at 9.943 kb on + strand at 10.253 kb on - strand at 10.254 kb on + strand at 10.283 kb on - strand at 10.338 kb on + strand at 10.490 kb on + strand, within GFF3662 at 10.553 kb on + strand, within GFF3662 at 10.572 kb on + strand, within GFF3662 at 10.649 kb on - strand, within GFF3662 at 10.680 kb on + strand, within GFF3662 at 10.680 kb on + strand, within GFF3662 at 10.680 kb on + strand, within GFF3662 at 10.791 kb on + strand, within GFF3662 at 10.908 kb on + strand, within GFF3662 at 10.919 kb on + strand, within GFF3662 at 10.950 kb on - strand, within GFF3662 at 10.950 kb on - strand, within GFF3662 at 11.110 kb on - strand, within GFF3662 at 11.302 kb on - strand, within GFF3662 at 11.422 kb on - strand, within GFF3662 at 11.528 kb on + strand, within GFF3662 at 11.639 kb on - strand, within GFF3662 at 11.639 kb on - strand, within GFF3662 at 11.811 kb on - strand, within GFF3662 at 11.896 kb on + strand at 11.919 kb on + strand at 11.919 kb on + strand at 11.919 kb on + strand at 12.046 kb on - strand at 12.106 kb on + strand at 12.106 kb on + strand at 12.120 kb on + strand at 12.152 kb on - strand at 12.271 kb on + strand, within GFF3663 at 12.271 kb on + strand, within GFF3663 at 12.279 kb on - strand, within GFF3663 at 12.435 kb on + strand, within GFF3663 at 12.436 kb on - strand, within GFF3663 at 12.829 kb on - strand at 13.021 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 9,349 - GFF3661 0.38 -0.4 9,413 - GFF3661 0.46 -0.8 9,481 + GFF3661 0.54 +0.4 9,482 - GFF3661 0.54 -1.0 9,484 - GFF3661 0.55 -0.1 9,543 + GFF3661 0.62 +0.6 9,593 - GFF3661 0.68 +0.3 9,593 - GFF3661 0.68 +0.2 9,603 - GFF3661 0.69 -0.1 9,943 + +0.8 10,253 - +0.2 10,254 + +0.2 10,283 - -0.3 10,338 + +0.6 10,490 + GFF3662 0.11 -1.0 10,553 + GFF3662 0.14 +0.0 10,572 + GFF3662 0.15 -0.7 10,649 - GFF3662 0.20 -0.5 10,680 + GFF3662 0.22 +0.9 10,680 + GFF3662 0.22 +1.1 10,680 + GFF3662 0.22 +0.1 10,791 + GFF3662 0.28 -1.3 10,908 + GFF3662 0.35 -0.9 10,919 + GFF3662 0.35 -0.7 10,950 - GFF3662 0.37 -0.5 10,950 - GFF3662 0.37 -1.0 11,110 - GFF3662 0.46 +0.3 11,302 - GFF3662 0.57 +0.3 11,422 - GFF3662 0.64 -0.5 11,528 + GFF3662 0.70 -0.3 11,639 - GFF3662 0.76 +0.4 11,639 - GFF3662 0.76 +0.3 11,811 - GFF3662 0.86 -0.1 11,896 + -1.4 11,919 + -0.0 11,919 + -0.8 11,919 + -0.1 12,046 - +0.0 12,106 + +0.0 12,106 + +0.0 12,120 + +0.2 12,152 - +0.1 12,271 + GFF3663 0.11 +1.0 12,271 + GFF3663 0.11 -0.2 12,279 - GFF3663 0.12 +0.9 12,435 + GFF3663 0.34 -1.3 12,436 - GFF3663 0.34 +1.3 12,829 - +0.4 13,021 + -0.2
Or see this region's nucleotide sequence