Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF358 and GFF359 are separated by 54 nucleotides GFF359 and GFF360 are separated by 64 nucleotides GFF360 and GFF361 are separated by 42 nucleotides GFF361 and GFF362 overlap by 4 nucleotides GFF362 and GFF363 are separated by 250 nucleotides
GFF358 - DNA-directed RNA polymerase omega subunit (EC 2.7.7.6), at 79,027 to 79,302
GFF358
GFF359 - Guanylate kinase (EC 2.7.4.8), at 79,357 to 79,980
GFF359
GFF360 - hypothetical protein, at 80,045 to 80,194
GFF360
GFF361 - DNA ligase (EC 6.5.1.2), LigB, at 80,237 to 81,922
GFF361
GFF362 - Putative inner membrane protein, at 81,919 to 82,536
GFF362
GFF363 - Putative beta-lactamase, at 82,787 to 83,668
GFF363
Position (kb)
80
81
82 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 79.303 kb on - strand at 79.307 kb on - strand at 79.414 kb on - strand at 80.029 kb on - strand at 80.029 kb on - strand at 80.029 kb on - strand at 80.033 kb on + strand at 80.056 kb on - strand at 80.175 kb on - strand, within GFF360 at 80.195 kb on + strand at 80.254 kb on + strand at 80.542 kb on + strand, within GFF361 at 80.565 kb on + strand, within GFF361 at 80.565 kb on + strand, within GFF361 at 80.628 kb on + strand, within GFF361 at 80.728 kb on + strand, within GFF361 at 80.728 kb on + strand, within GFF361 at 80.729 kb on - strand, within GFF361 at 80.769 kb on + strand, within GFF361 at 80.788 kb on - strand, within GFF361 at 80.803 kb on + strand, within GFF361 at 80.803 kb on + strand, within GFF361 at 80.804 kb on - strand, within GFF361 at 80.902 kb on + strand, within GFF361 at 80.946 kb on + strand, within GFF361 at 80.987 kb on + strand, within GFF361 at 80.987 kb on + strand, within GFF361 at 80.989 kb on + strand, within GFF361 at 81.075 kb on + strand, within GFF361 at 81.082 kb on - strand, within GFF361 at 81.082 kb on - strand, within GFF361 at 81.152 kb on + strand, within GFF361 at 81.225 kb on + strand, within GFF361 at 81.229 kb on - strand, within GFF361 at 81.229 kb on - strand, within GFF361 at 81.259 kb on + strand, within GFF361 at 81.404 kb on - strand, within GFF361 at 81.580 kb on + strand, within GFF361 at 81.580 kb on + strand, within GFF361 at 81.657 kb on + strand, within GFF361 at 81.658 kb on - strand, within GFF361 at 81.775 kb on - strand at 81.777 kb on + strand at 81.807 kb on + strand at 81.856 kb on + strand at 81.902 kb on - strand at 81.920 kb on + strand at 81.920 kb on + strand at 81.921 kb on - strand at 81.921 kb on - strand at 81.980 kb on - strand at 81.980 kb on - strand at 81.990 kb on + strand, within GFF362 at 82.003 kb on + strand, within GFF362 at 82.004 kb on - strand, within GFF362 at 82.009 kb on - strand, within GFF362 at 82.014 kb on + strand, within GFF362 at 82.041 kb on - strand, within GFF362 at 82.058 kb on + strand, within GFF362 at 82.059 kb on - strand, within GFF362 at 82.059 kb on - strand, within GFF362 at 82.059 kb on - strand, within GFF362 at 82.059 kb on - strand, within GFF362 at 82.059 kb on - strand, within GFF362 at 82.093 kb on + strand, within GFF362 at 82.093 kb on + strand, within GFF362 at 82.094 kb on - strand, within GFF362 at 82.094 kb on - strand, within GFF362 at 82.094 kb on - strand, within GFF362 at 82.094 kb on - strand, within GFF362 at 82.094 kb on - strand, within GFF362 at 82.134 kb on + strand, within GFF362 at 82.165 kb on - strand, within GFF362 at 82.217 kb on - strand, within GFF362 at 82.217 kb on - strand, within GFF362 at 82.217 kb on - strand, within GFF362 at 82.283 kb on - strand, within GFF362 at 82.338 kb on + strand, within GFF362 at 82.339 kb on - strand, within GFF362 at 82.346 kb on + strand, within GFF362 at 82.434 kb on + strand, within GFF362 at 82.438 kb on - strand, within GFF362 at 82.438 kb on - strand, within GFF362 at 82.438 kb on - strand, within GFF362 at 82.513 kb on + strand at 82.513 kb on + strand at 82.780 kb on + strand at 82.781 kb on - strand at 82.842 kb on + strand at 82.843 kb on - strand at 82.843 kb on - strand at 82.843 kb on - strand at 82.879 kb on + strand, within GFF363
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 79,303 - -1.6 79,307 - -1.2 79,414 - +0.3 80,029 - -0.8 80,029 - +0.3 80,029 - -0.8 80,033 + -0.4 80,056 - -0.1 80,175 - GFF360 0.87 -2.9 80,195 + -1.7 80,254 + -0.0 80,542 + GFF361 0.18 -0.7 80,565 + GFF361 0.19 +0.1 80,565 + GFF361 0.19 +0.0 80,628 + GFF361 0.23 -0.5 80,728 + GFF361 0.29 -0.2 80,728 + GFF361 0.29 -0.4 80,729 - GFF361 0.29 -0.8 80,769 + GFF361 0.32 -1.7 80,788 - GFF361 0.33 -0.1 80,803 + GFF361 0.34 -1.1 80,803 + GFF361 0.34 -2.5 80,804 - GFF361 0.34 +0.3 80,902 + GFF361 0.39 -0.8 80,946 + GFF361 0.42 -0.6 80,987 + GFF361 0.44 -1.3 80,987 + GFF361 0.44 -0.3 80,989 + GFF361 0.45 -1.2 81,075 + GFF361 0.50 +0.1 81,082 - GFF361 0.50 -0.4 81,082 - GFF361 0.50 -0.9 81,152 + GFF361 0.54 -0.3 81,225 + GFF361 0.59 -0.7 81,229 - GFF361 0.59 +0.6 81,229 - GFF361 0.59 +1.9 81,259 + GFF361 0.61 -0.4 81,404 - GFF361 0.69 -0.1 81,580 + GFF361 0.80 -0.2 81,580 + GFF361 0.80 -0.2 81,657 + GFF361 0.84 +0.3 81,658 - GFF361 0.84 -0.6 81,775 - -0.3 81,777 + -0.7 81,807 + +0.6 81,856 + -1.3 81,902 - -0.4 81,920 + -1.5 81,920 + -0.2 81,921 - -0.5 81,921 - -0.1 81,980 - +0.2 81,980 - +0.5 81,990 + GFF362 0.11 +0.1 82,003 + GFF362 0.14 -0.9 82,004 - GFF362 0.14 +0.1 82,009 - GFF362 0.15 +3.0 82,014 + GFF362 0.15 -0.8 82,041 - GFF362 0.20 +1.3 82,058 + GFF362 0.22 +0.5 82,059 - GFF362 0.23 -0.6 82,059 - GFF362 0.23 +0.5 82,059 - GFF362 0.23 -0.5 82,059 - GFF362 0.23 -0.5 82,059 - GFF362 0.23 -0.3 82,093 + GFF362 0.28 -0.3 82,093 + GFF362 0.28 -0.4 82,094 - GFF362 0.28 -0.6 82,094 - GFF362 0.28 +0.2 82,094 - GFF362 0.28 -0.5 82,094 - GFF362 0.28 +0.1 82,094 - GFF362 0.28 -1.1 82,134 + GFF362 0.35 -0.4 82,165 - GFF362 0.40 -0.0 82,217 - GFF362 0.48 -0.1 82,217 - GFF362 0.48 -0.4 82,217 - GFF362 0.48 -0.3 82,283 - GFF362 0.59 -0.2 82,338 + GFF362 0.68 -0.8 82,339 - GFF362 0.68 +0.5 82,346 + GFF362 0.69 +1.2 82,434 + GFF362 0.83 -0.2 82,438 - GFF362 0.84 +0.7 82,438 - GFF362 0.84 +0.6 82,438 - GFF362 0.84 -1.3 82,513 + +0.4 82,513 + +0.4 82,780 + +0.3 82,781 - -0.4 82,842 + +1.0 82,843 - -0.9 82,843 - -0.6 82,843 - -0.6 82,879 + GFF363 0.10 -0.7
Or see this region's nucleotide sequence