Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3527 and GFF3528 are separated by 4 nucleotides GFF3528 and GFF3529 are separated by 202 nucleotides GFF3529 and GFF3530 are separated by 91 nucleotides GFF3530 and GFF3531 are separated by 11 nucleotides
GFF3527 - Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20), at 278,730 to 281,117
GFF3527
GFF3528 - Integration host factor alpha subunit, at 281,122 to 281,421
GFF3528
GFF3529 - Vitamin B12 ABC transporter, permease component BtuC, at 281,624 to 282,604
GFF3529
GFF3530 - Glutathione peroxidase family protein, at 282,696 to 283,247
GFF3530
GFF3531 - Vitamin B12 ABC transporter, ATPase component BtuD, at 283,259 to 283,996
GFF3531
Position (kb) 281
282
283 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 281.272 kb on - strand, within GFF3528 at 281.331 kb on + strand, within GFF3528 at 281.337 kb on + strand, within GFF3528 at 281.452 kb on + strand at 281.452 kb on + strand at 281.464 kb on + strand at 281.465 kb on - strand at 281.484 kb on - strand at 281.504 kb on + strand at 281.504 kb on + strand at 281.505 kb on - strand at 281.715 kb on + strand at 282.007 kb on + strand, within GFF3529 at 282.086 kb on - strand, within GFF3529 at 282.152 kb on + strand, within GFF3529 at 282.421 kb on - strand, within GFF3529 at 282.487 kb on - strand, within GFF3529 at 282.642 kb on - strand at 282.648 kb on - strand at 282.750 kb on - strand at 283.245 kb on + strand at 283.371 kb on + strand, within GFF3531 at 283.371 kb on + strand, within GFF3531 at 283.382 kb on - strand, within GFF3531
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 281,272 - GFF3528 0.50 +3.4 281,331 + GFF3528 0.70 +3.4 281,337 + GFF3528 0.72 +4.4 281,452 + +1.7 281,452 + +2.0 281,464 + +2.1 281,465 - +3.2 281,484 - +4.1 281,504 + +0.7 281,504 + +0.1 281,505 - +1.5 281,715 + +1.0 282,007 + GFF3529 0.39 -0.3 282,086 - GFF3529 0.47 +0.0 282,152 + GFF3529 0.54 +2.2 282,421 - GFF3529 0.81 +1.0 282,487 - GFF3529 0.88 -0.1 282,642 - +0.3 282,648 - -0.6 282,750 - -1.5 283,245 + -1.5 283,371 + GFF3531 0.15 +0.8 283,371 + GFF3531 0.15 +1.4 283,382 - GFF3531 0.17 -0.7
Or see this region's nucleotide sequence