Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3382 and GFF3383 are separated by 23 nucleotides GFF3383 and GFF3384 are separated by 133 nucleotides GFF3384 and GFF3385 are separated by 84 nucleotides
GFF3382 - Putative deoxyribonuclease YcfH, at 147,329 to 148,126
GFF3382
GFF3383 - hypothetical protein, at 148,150 to 148,287
GFF3383
GFF3384 - PTS system, glucose-specific IIB component (EC 2.7.1.69) / PTS system, glucose-specific IIC component (EC 2.7.1.69), at 148,421 to 149,854
GFF3384
GFF3385 - Putative OMR family iron-siderophore receptor precursor, at 149,939 to 152,113
GFF3385
Position (kb) 148
149
150 Strain fitness (log2 ratio) -1
0
1
2 at 147.551 kb on + strand, within GFF3382 at 147.624 kb on - strand, within GFF3382 at 147.668 kb on - strand, within GFF3382 at 147.739 kb on + strand, within GFF3382 at 147.786 kb on + strand, within GFF3382 at 147.790 kb on - strand, within GFF3382 at 147.845 kb on + strand, within GFF3382 at 147.927 kb on + strand, within GFF3382 at 147.928 kb on - strand, within GFF3382 at 147.930 kb on + strand, within GFF3382 at 147.950 kb on + strand, within GFF3382 at 148.016 kb on + strand, within GFF3382 at 148.093 kb on + strand at 148.307 kb on - strand at 148.401 kb on - strand at 148.427 kb on - strand at 148.786 kb on + strand, within GFF3384 at 148.787 kb on - strand, within GFF3384 at 148.787 kb on - strand, within GFF3384 at 148.808 kb on + strand, within GFF3384 at 148.809 kb on - strand, within GFF3384 at 148.809 kb on - strand, within GFF3384 at 148.809 kb on - strand, within GFF3384 at 148.810 kb on + strand, within GFF3384 at 148.848 kb on - strand, within GFF3384 at 149.165 kb on - strand, within GFF3384 at 149.360 kb on - strand, within GFF3384 at 149.399 kb on - strand, within GFF3384 at 149.399 kb on - strand, within GFF3384 at 149.423 kb on - strand, within GFF3384 at 149.669 kb on - strand, within GFF3384 at 149.846 kb on - strand at 149.852 kb on + strand at 150.102 kb on + strand at 150.146 kb on - strand at 150.254 kb on - strand, within GFF3385 at 150.328 kb on + strand, within GFF3385 at 150.442 kb on + strand, within GFF3385 at 150.520 kb on - strand, within GFF3385 at 150.523 kb on + strand, within GFF3385 at 150.592 kb on + strand, within GFF3385 at 150.592 kb on + strand, within GFF3385 at 150.593 kb on - strand, within GFF3385 at 150.630 kb on + strand, within GFF3385 at 150.703 kb on + strand, within GFF3385 at 150.703 kb on + strand, within GFF3385 at 150.704 kb on - strand, within GFF3385 at 150.763 kb on - strand, within GFF3385 at 150.784 kb on + strand, within GFF3385
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 147,551 + GFF3382 0.28 +1.5 147,624 - GFF3382 0.37 +0.1 147,668 - GFF3382 0.42 +0.4 147,739 + GFF3382 0.51 +1.0 147,786 + GFF3382 0.57 +0.8 147,790 - GFF3382 0.58 -0.4 147,845 + GFF3382 0.65 +0.1 147,927 + GFF3382 0.75 +1.1 147,928 - GFF3382 0.75 +0.6 147,930 + GFF3382 0.75 -0.5 147,950 + GFF3382 0.78 +0.8 148,016 + GFF3382 0.86 +0.8 148,093 + +1.2 148,307 - +0.2 148,401 - +1.0 148,427 - +1.1 148,786 + GFF3384 0.25 +1.0 148,787 - GFF3384 0.26 +0.8 148,787 - GFF3384 0.26 +1.7 148,808 + GFF3384 0.27 +0.6 148,809 - GFF3384 0.27 +1.1 148,809 - GFF3384 0.27 +0.8 148,809 - GFF3384 0.27 +0.9 148,810 + GFF3384 0.27 +0.3 148,848 - GFF3384 0.30 +1.0 149,165 - GFF3384 0.52 +1.5 149,360 - GFF3384 0.65 +0.9 149,399 - GFF3384 0.68 -0.2 149,399 - GFF3384 0.68 +0.3 149,423 - GFF3384 0.70 +1.2 149,669 - GFF3384 0.87 +0.1 149,846 - +0.5 149,852 + +0.8 150,102 + +0.4 150,146 - +2.4 150,254 - GFF3385 0.14 -0.1 150,328 + GFF3385 0.18 +0.8 150,442 + GFF3385 0.23 -1.2 150,520 - GFF3385 0.27 -0.1 150,523 + GFF3385 0.27 +0.4 150,592 + GFF3385 0.30 +2.3 150,592 + GFF3385 0.30 +0.1 150,593 - GFF3385 0.30 +1.0 150,630 + GFF3385 0.32 -0.4 150,703 + GFF3385 0.35 +0.3 150,703 + GFF3385 0.35 -0.1 150,704 - GFF3385 0.35 +0.3 150,763 - GFF3385 0.38 +0.3 150,784 + GFF3385 0.39 -0.7
Or see this region's nucleotide sequence