Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3359 and GFF3360 are separated by 87 nucleotides GFF3360 and GFF3361 are separated by 11 nucleotides GFF3361 and GFF3362 overlap by 1 nucleotides
GFF3359 - Flagellar basal-body rod protein FlgG, at 123,861 to 124,643
GFF3359
GFF3360 - Flagellar L-ring protein FlgH, at 124,731 to 125,396
GFF3360
GFF3361 - Flagellar P-ring protein FlgI, at 125,408 to 126,505
GFF3361
GFF3362 - Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-), at 126,505 to 127,455
GFF3362
Position (kb) 125
126
127 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 124.435 kb on - strand, within GFF3359 at 124.503 kb on + strand, within GFF3359 at 124.512 kb on + strand, within GFF3359 at 124.779 kb on - strand at 124.871 kb on + strand, within GFF3360 at 124.875 kb on + strand, within GFF3360 at 124.882 kb on - strand, within GFF3360 at 125.044 kb on + strand, within GFF3360 at 125.180 kb on - strand, within GFF3360 at 125.180 kb on - strand, within GFF3360 at 125.316 kb on + strand, within GFF3360 at 125.316 kb on + strand, within GFF3360 at 125.394 kb on + strand at 125.395 kb on - strand at 125.395 kb on - strand at 125.405 kb on + strand at 125.405 kb on + strand at 125.406 kb on - strand at 125.406 kb on - strand at 125.641 kb on + strand, within GFF3361 at 125.642 kb on - strand, within GFF3361 at 125.773 kb on - strand, within GFF3361 at 125.784 kb on + strand, within GFF3361 at 125.807 kb on + strand, within GFF3361 at 125.808 kb on - strand, within GFF3361 at 125.808 kb on - strand, within GFF3361 at 125.827 kb on + strand, within GFF3361 at 125.888 kb on - strand, within GFF3361 at 125.962 kb on + strand, within GFF3361 at 126.076 kb on - strand, within GFF3361 at 126.130 kb on + strand, within GFF3361 at 126.220 kb on + strand, within GFF3361 at 126.481 kb on - strand at 126.520 kb on - strand at 127.220 kb on - strand, within GFF3362 at 127.241 kb on + strand, within GFF3362 at 127.280 kb on - strand, within GFF3362 at 127.454 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 124,435 - GFF3359 0.73 +1.0 124,503 + GFF3359 0.82 +1.4 124,512 + GFF3359 0.83 +1.6 124,779 - +1.1 124,871 + GFF3360 0.21 +0.9 124,875 + GFF3360 0.22 +1.1 124,882 - GFF3360 0.23 +1.2 125,044 + GFF3360 0.47 +4.0 125,180 - GFF3360 0.67 +2.4 125,180 - GFF3360 0.67 +1.9 125,316 + GFF3360 0.88 +1.1 125,316 + GFF3360 0.88 +0.3 125,394 + +0.3 125,395 - +2.6 125,395 - +1.0 125,405 + +1.2 125,405 + +0.8 125,406 - +0.6 125,406 - +0.0 125,641 + GFF3361 0.21 -0.2 125,642 - GFF3361 0.21 +1.4 125,773 - GFF3361 0.33 +1.2 125,784 + GFF3361 0.34 +1.8 125,807 + GFF3361 0.36 +1.9 125,808 - GFF3361 0.36 +0.9 125,808 - GFF3361 0.36 +2.0 125,827 + GFF3361 0.38 -0.3 125,888 - GFF3361 0.44 +0.4 125,962 + GFF3361 0.50 +1.0 126,076 - GFF3361 0.61 +1.7 126,130 + GFF3361 0.66 +0.3 126,220 + GFF3361 0.74 +0.4 126,481 - +2.2 126,520 - +1.7 127,220 - GFF3362 0.75 +1.6 127,241 + GFF3362 0.77 +1.0 127,280 - GFF3362 0.81 +1.0 127,454 - -1.4
Or see this region's nucleotide sequence