Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3292 and GFF3293 overlap by 11 nucleotides GFF3293 and GFF3294 are separated by 132 nucleotides GFF3294 and GFF3295 are separated by 33 nucleotides GFF3295 and GFF3296 are separated by 20 nucleotides
GFF3292 - Secreted protein, suppressor for copper-sensitivity ScsC, at 64,314 to 64,937
GFF3292
GFF3293 - Membrane protein, suppressor for copper-sensitivity ScsD, at 64,927 to 65,433
GFF3293
GFF3294 - Glucose-1-phosphatase (EC 3.1.3.10), at 65,566 to 66,807
GFF3294
GFF3295 - FIG00895798: hypothetical protein, at 66,841 to 67,068
GFF3295
GFF3296 - Flavoprotein WrbA, at 67,089 to 67,685
GFF3296
Position (kb) 65
66
67 Strain fitness (log2 ratio) -1
0
1
2 at 64.581 kb on - strand, within GFF3292 at 64.599 kb on - strand, within GFF3292 at 64.705 kb on - strand, within GFF3292 at 64.721 kb on + strand, within GFF3292 at 65.054 kb on + strand, within GFF3293 at 65.055 kb on - strand, within GFF3293 at 65.058 kb on + strand, within GFF3293 at 65.059 kb on - strand, within GFF3293 at 65.154 kb on + strand, within GFF3293 at 65.157 kb on + strand, within GFF3293 at 65.158 kb on - strand, within GFF3293 at 65.390 kb on - strand at 65.472 kb on + strand at 65.561 kb on + strand at 65.562 kb on - strand at 65.658 kb on - strand at 65.658 kb on - strand at 65.806 kb on + strand, within GFF3294 at 65.885 kb on + strand, within GFF3294 at 65.885 kb on + strand, within GFF3294 at 65.886 kb on - strand, within GFF3294 at 65.886 kb on - strand, within GFF3294 at 65.886 kb on - strand, within GFF3294 at 65.887 kb on + strand, within GFF3294 at 65.887 kb on + strand, within GFF3294 at 65.888 kb on - strand, within GFF3294 at 65.888 kb on - strand, within GFF3294 at 65.997 kb on + strand, within GFF3294 at 66.021 kb on - strand, within GFF3294 at 66.024 kb on + strand, within GFF3294 at 66.089 kb on + strand, within GFF3294 at 66.090 kb on - strand, within GFF3294 at 66.090 kb on - strand, within GFF3294 at 66.270 kb on + strand, within GFF3294 at 66.280 kb on + strand, within GFF3294 at 66.283 kb on + strand, within GFF3294 at 66.434 kb on - strand, within GFF3294 at 66.562 kb on + strand, within GFF3294 at 66.646 kb on + strand, within GFF3294 at 66.812 kb on - strand at 66.812 kb on - strand at 66.843 kb on - strand at 66.844 kb on + strand at 67.011 kb on + strand, within GFF3295 at 67.012 kb on - strand, within GFF3295 at 67.012 kb on - strand, within GFF3295 at 67.579 kb on - strand, within GFF3296 at 67.581 kb on - strand, within GFF3296 at 67.690 kb on - strand at 67.705 kb on - strand at 67.733 kb on + strand at 67.734 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 64,581 - GFF3292 0.43 +1.8 64,599 - GFF3292 0.46 +0.9 64,705 - GFF3292 0.63 +1.2 64,721 + GFF3292 0.65 -0.3 65,054 + GFF3293 0.25 +1.6 65,055 - GFF3293 0.25 +1.2 65,058 + GFF3293 0.26 -0.1 65,059 - GFF3293 0.26 +0.9 65,154 + GFF3293 0.45 +1.9 65,157 + GFF3293 0.45 -0.2 65,158 - GFF3293 0.46 +0.3 65,390 - -0.6 65,472 + -0.4 65,561 + +0.1 65,562 - -0.0 65,658 - +1.4 65,658 - +0.5 65,806 + GFF3294 0.19 -0.8 65,885 + GFF3294 0.26 -0.0 65,885 + GFF3294 0.26 -0.8 65,886 - GFF3294 0.26 -1.2 65,886 - GFF3294 0.26 -0.2 65,886 - GFF3294 0.26 +0.6 65,887 + GFF3294 0.26 +0.3 65,887 + GFF3294 0.26 -0.4 65,888 - GFF3294 0.26 +1.1 65,888 - GFF3294 0.26 +0.1 65,997 + GFF3294 0.35 -0.5 66,021 - GFF3294 0.37 +0.6 66,024 + GFF3294 0.37 +0.3 66,089 + GFF3294 0.42 -0.5 66,090 - GFF3294 0.42 +1.1 66,090 - GFF3294 0.42 +0.8 66,270 + GFF3294 0.57 -0.3 66,280 + GFF3294 0.57 +0.2 66,283 + GFF3294 0.58 +1.4 66,434 - GFF3294 0.70 +0.6 66,562 + GFF3294 0.80 +0.4 66,646 + GFF3294 0.87 -0.4 66,812 - +0.4 66,812 - -0.7 66,843 - +0.4 66,844 + +1.5 67,011 + GFF3295 0.75 +0.1 67,012 - GFF3295 0.75 -0.9 67,012 - GFF3295 0.75 -0.1 67,579 - GFF3296 0.82 +1.4 67,581 - GFF3296 0.82 -0.0 67,690 - +0.4 67,705 - -0.3 67,733 + -0.5 67,734 - -0.6
Or see this region's nucleotide sequence