Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3275

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3273 and GFF3274 are separated by 135 nucleotidesGFF3274 and GFF3275 are separated by 332 nucleotidesGFF3275 and GFF3276 are separated by 17 nucleotides GFF3273 - Putative two-component system response regulator YedW, at 45,503 to 46,183 GFF3273 GFF3274 - 5-Hydroxyisourate Hydrolase (HIUase) (EC 3.5.2.17), at 46,319 to 46,729 GFF3274 GFF3275 - 4-hydroxyphenylacetate 3-monooxygenase, reductase component (EC 1.6.8.-), at 47,062 to 47,574 GFF3275 GFF3276 - 4-hydroxyphenylacetate 3-monooxygenase (EC 1.14.13.3), at 47,592 to 49,154 GFF3276 Position (kb) 47 48Strain fitness (log2 ratio) -3 -2 -1 0 1at 46.099 kb on - strand, within GFF3273at 46.111 kb on - strand, within GFF3273at 46.192 kb on + strandat 46.240 kb on + strandat 46.240 kb on + strandat 46.241 kb on - strandat 46.284 kb on + strandat 46.284 kb on + strandat 46.284 kb on + strandat 46.330 kb on + strandat 46.330 kb on + strandat 46.331 kb on - strandat 46.331 kb on - strandat 46.332 kb on + strandat 46.348 kb on - strandat 46.348 kb on - strandat 46.411 kb on + strand, within GFF3274at 46.412 kb on - strand, within GFF3274at 46.525 kb on + strand, within GFF3274at 46.526 kb on - strand, within GFF3274at 46.569 kb on - strand, within GFF3274at 46.690 kb on - strandat 46.707 kb on - strandat 46.933 kb on + strandat 47.008 kb on - strandat 47.140 kb on - strand, within GFF3275at 47.149 kb on - strand, within GFF3275at 47.194 kb on + strand, within GFF3275at 47.235 kb on + strand, within GFF3275at 47.347 kb on + strand, within GFF3275at 47.349 kb on + strand, within GFF3275at 47.349 kb on + strand, within GFF3275at 47.350 kb on - strand, within GFF3275at 47.350 kb on - strand, within GFF3275at 47.353 kb on + strand, within GFF3275at 47.353 kb on + strand, within GFF3275at 47.358 kb on + strand, within GFF3275at 47.415 kb on + strand, within GFF3275at 47.415 kb on + strand, within GFF3275at 47.415 kb on + strand, within GFF3275at 47.416 kb on - strand, within GFF3275at 47.420 kb on - strand, within GFF3275at 47.630 kb on + strandat 47.706 kb on - strandat 47.712 kb on - strandat 47.838 kb on - strand, within GFF3276at 47.998 kb on + strand, within GFF3276at 48.089 kb on + strand, within GFF3276at 48.090 kb on - strand, within GFF3276at 48.253 kb on + strand, within GFF3276at 48.315 kb on + strand, within GFF3276at 48.316 kb on - strand, within GFF3276at 48.433 kb on + strand, within GFF3276at 48.547 kb on + strand, within GFF3276at 48.548 kb on - strand, within GFF3276

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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46,099 - GFF3273 0.88 +1.1
46,111 - GFF3273 0.89 -0.1
46,192 + -0.7
46,240 + -0.1
46,240 + +0.0
46,241 - -0.2
46,284 + -0.8
46,284 + +0.3
46,284 + -2.1
46,330 + -0.3
46,330 + -0.2
46,331 - +0.0
46,331 - -0.1
46,332 + +0.1
46,348 - +0.3
46,348 - -0.7
46,411 + GFF3274 0.22 -0.7
46,412 - GFF3274 0.23 -1.3
46,525 + GFF3274 0.50 -0.4
46,526 - GFF3274 0.50 -0.5
46,569 - GFF3274 0.61 -0.6
46,690 - -0.1
46,707 - +1.3
46,933 + -0.0
47,008 - +0.9
47,140 - GFF3275 0.15 -0.2
47,149 - GFF3275 0.17 -1.7
47,194 + GFF3275 0.26 -0.5
47,235 + GFF3275 0.34 -0.5
47,347 + GFF3275 0.56 -3.1
47,349 + GFF3275 0.56 -0.5
47,349 + GFF3275 0.56 +0.1
47,350 - GFF3275 0.56 -0.3
47,350 - GFF3275 0.56 -0.4
47,353 + GFF3275 0.57 +0.6
47,353 + GFF3275 0.57 -0.0
47,358 + GFF3275 0.58 -0.8
47,415 + GFF3275 0.69 +0.4
47,415 + GFF3275 0.69 -0.2
47,415 + GFF3275 0.69 +0.2
47,416 - GFF3275 0.69 +0.2
47,420 - GFF3275 0.70 -0.1
47,630 + +0.4
47,706 - -1.2
47,712 - -1.6
47,838 - GFF3276 0.16 -0.4
47,998 + GFF3276 0.26 -1.1
48,089 + GFF3276 0.32 +0.5
48,090 - GFF3276 0.32 -1.2
48,253 + GFF3276 0.42 +0.8
48,315 + GFF3276 0.46 +0.4
48,316 - GFF3276 0.46 +0.2
48,433 + GFF3276 0.54 +0.6
48,547 + GFF3276 0.61 -0.2
48,548 - GFF3276 0.61 -0.6

Or see this region's nucleotide sequence