Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3249

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3248 and GFF3249 are separated by 332 nucleotidesGFF3249 and GFF3250 are separated by 216 nucleotidesGFF3250 and GFF3251 overlap by 14 nucleotides GFF3248 - Outer membrane protein A precursor, at 22,831 to 23,907 GFF3248 GFF3249 - Cell division inhibitor, at 24,240 to 24,749 GFF3249 GFF3250 - DNA transformation protein TfoX, at 24,966 to 25,571 GFF3250 GFF3251 - Putative efflux (PET) family inner membrane protein YccS, at 25,558 to 27,711 GFF3251 Position (kb) 24 25Strain fitness (log2 ratio) -2 -1 0 1at 23.243 kb on - strand, within GFF3248at 23.318 kb on - strand, within GFF3248at 23.473 kb on - strand, within GFF3248at 23.519 kb on - strand, within GFF3248at 23.605 kb on - strand, within GFF3248at 23.658 kb on - strand, within GFF3248at 23.831 kb on - strandat 23.988 kb on - strandat 23.988 kb on - strandat 23.992 kb on - strandat 24.022 kb on - strandat 24.036 kb on - strandat 24.048 kb on - strandat 24.093 kb on + strandat 24.121 kb on - strandat 24.181 kb on + strandat 24.339 kb on + strand, within GFF3249at 24.405 kb on + strand, within GFF3249at 24.406 kb on - strand, within GFF3249at 24.416 kb on - strand, within GFF3249at 24.426 kb on - strand, within GFF3249at 24.580 kb on - strand, within GFF3249at 24.746 kb on - strandat 24.749 kb on + strandat 24.750 kb on - strandat 24.750 kb on - strandat 25.207 kb on - strand, within GFF3250at 25.209 kb on + strand, within GFF3250at 25.266 kb on + strand, within GFF3250at 25.309 kb on + strand, within GFF3250at 25.337 kb on + strand, within GFF3250at 25.392 kb on - strand, within GFF3250at 25.392 kb on - strand, within GFF3250at 25.471 kb on - strand, within GFF3250at 25.499 kb on - strand, within GFF3250at 25.573 kb on + strandat 25.574 kb on - strandat 25.574 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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23,243 - GFF3248 0.38 +0.4
23,318 - GFF3248 0.45 -0.1
23,473 - GFF3248 0.60 +0.3
23,519 - GFF3248 0.64 -1.8
23,605 - GFF3248 0.72 +0.4
23,658 - GFF3248 0.77 -0.7
23,831 - +0.9
23,988 - +0.1
23,988 - -0.5
23,992 - -2.1
24,022 - +0.4
24,036 - -0.2
24,048 - -0.2
24,093 + -0.2
24,121 - +0.7
24,181 + -0.4
24,339 + GFF3249 0.19 -2.7
24,405 + GFF3249 0.32 +0.1
24,406 - GFF3249 0.33 -2.4
24,416 - GFF3249 0.35 -0.0
24,426 - GFF3249 0.36 -1.2
24,580 - GFF3249 0.67 +0.1
24,746 - -2.6
24,749 + +0.2
24,750 - +0.4
24,750 - -0.9
25,207 - GFF3250 0.40 +0.9
25,209 + GFF3250 0.40 +0.8
25,266 + GFF3250 0.50 -0.1
25,309 + GFF3250 0.57 -0.8
25,337 + GFF3250 0.61 -0.6
25,392 - GFF3250 0.70 +0.4
25,392 - GFF3250 0.70 +1.1
25,471 - GFF3250 0.83 -0.1
25,499 - GFF3250 0.88 -1.0
25,573 + -0.0
25,574 - -0.8
25,574 - +1.4

Or see this region's nucleotide sequence