Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3031

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3030 and GFF3031 are separated by 204 nucleotidesGFF3031 and GFF3032 are separated by 279 nucleotidesGFF3032 and GFF3033 are separated by 38 nucleotides GFF3030 - Agmatinase (EC 3.5.3.11), at 44,653 to 45,573 GFF3030 GFF3031 - FIG01048344: hypothetical protein, at 45,778 to 46,668 GFF3031 GFF3032 - Putative mannitol dehydrogenase, at 46,948 to 47,421 GFF3032 GFF3033 - Malate dehydrogenase (EC 1.1.1.37), at 47,460 to 48,467 GFF3033 Position (kb) 45 46 47Strain fitness (log2 ratio) -1 0 1at 44.897 kb on + strand, within GFF3030at 44.941 kb on - strand, within GFF3030at 45.071 kb on + strand, within GFF3030at 45.110 kb on + strand, within GFF3030at 45.219 kb on + strand, within GFF3030at 45.220 kb on - strand, within GFF3030at 45.297 kb on - strand, within GFF3030at 45.297 kb on - strand, within GFF3030at 45.667 kb on - strandat 45.707 kb on - strandat 45.711 kb on + strandat 45.760 kb on + strandat 45.779 kb on + strandat 45.827 kb on + strandat 45.925 kb on - strand, within GFF3031at 45.970 kb on + strand, within GFF3031at 45.970 kb on + strand, within GFF3031at 46.105 kb on - strand, within GFF3031at 46.205 kb on + strand, within GFF3031at 46.205 kb on + strand, within GFF3031at 46.258 kb on + strand, within GFF3031at 46.387 kb on - strand, within GFF3031at 46.387 kb on - strand, within GFF3031at 47.022 kb on + strand, within GFF3032at 47.078 kb on - strand, within GFF3032at 47.149 kb on + strand, within GFF3032at 47.421 kb on + strandat 47.583 kb on + strand, within GFF3033at 47.593 kb on - strand, within GFF3033

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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44,897 + GFF3030 0.26 +0.1
44,941 - GFF3030 0.31 +0.9
45,071 + GFF3030 0.45 -0.5
45,110 + GFF3030 0.50 -0.3
45,219 + GFF3030 0.61 -0.3
45,220 - GFF3030 0.62 -0.6
45,297 - GFF3030 0.70 +0.3
45,297 - GFF3030 0.70 -0.0
45,667 - -0.0
45,707 - -0.4
45,711 + +0.5
45,760 + -0.1
45,779 + -0.2
45,827 + -0.3
45,925 - GFF3031 0.16 -0.7
45,970 + GFF3031 0.22 -0.9
45,970 + GFF3031 0.22 -0.4
46,105 - GFF3031 0.37 -1.0
46,205 + GFF3031 0.48 -1.1
46,205 + GFF3031 0.48 -0.6
46,258 + GFF3031 0.54 -0.6
46,387 - GFF3031 0.68 -0.8
46,387 - GFF3031 0.68 -0.7
47,022 + GFF3032 0.16 +0.1
47,078 - GFF3032 0.27 +1.1
47,149 + GFF3032 0.42 +1.1
47,421 + -0.1
47,583 + GFF3033 0.12 -0.6
47,593 - GFF3033 0.13 +0.3

Or see this region's nucleotide sequence