Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3005

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3003 and GFF3004 are separated by 162 nucleotidesGFF3004 and GFF3005 are separated by 25 nucleotidesGFF3005 and GFF3006 are separated by 40 nucleotidesGFF3006 and GFF3007 are separated by 273 nucleotides GFF3003 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 17,271 to 20,144 GFF3003 GFF3004 - Glycine cleavage system H protein, at 20,307 to 20,696 GFF3004 GFF3005 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 20,722 to 21,816 GFF3005 GFF3006 - hypothetical protein, at 21,857 to 21,997 GFF3006 GFF3007 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (EC 1.14.13.-), at 22,271 to 23,437 GFF3007 Position (kb) 20 21 22Strain fitness (log2 ratio) -2 -1 0 1at 19.810 kb on + strand, within GFF3003at 19.810 kb on + strand, within GFF3003at 19.819 kb on + strand, within GFF3003at 19.879 kb on + strandat 19.879 kb on + strandat 19.894 kb on + strandat 19.895 kb on - strandat 19.913 kb on + strandat 20.624 kb on - strand, within GFF3004at 20.624 kb on - strand, within GFF3004at 20.624 kb on - strand, within GFF3004at 20.643 kb on - strand, within GFF3004at 20.702 kb on - strandat 20.849 kb on + strand, within GFF3005at 20.938 kb on - strand, within GFF3005at 20.938 kb on - strand, within GFF3005at 21.025 kb on - strand, within GFF3005at 21.087 kb on + strand, within GFF3005at 21.088 kb on - strand, within GFF3005at 21.234 kb on - strand, within GFF3005at 21.306 kb on + strand, within GFF3005at 21.306 kb on + strand, within GFF3005at 21.306 kb on + strand, within GFF3005at 21.307 kb on - strand, within GFF3005at 21.307 kb on - strand, within GFF3005at 21.307 kb on - strand, within GFF3005at 21.336 kb on + strand, within GFF3005at 21.477 kb on + strand, within GFF3005at 21.509 kb on + strand, within GFF3005at 21.512 kb on + strand, within GFF3005at 21.587 kb on - strand, within GFF3005at 21.614 kb on + strand, within GFF3005at 21.619 kb on + strand, within GFF3005at 21.729 kb on - strandat 21.844 kb on - strandat 22.483 kb on - strand, within GFF3007at 22.650 kb on + strand, within GFF3007at 22.697 kb on + strand, within GFF3007at 22.697 kb on + strand, within GFF3007at 22.724 kb on - strand, within GFF3007at 22.766 kb on - strand, within GFF3007at 22.766 kb on - strand, within GFF3007

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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19,810 + GFF3003 0.88 +0.1
19,810 + GFF3003 0.88 +0.4
19,819 + GFF3003 0.89 +0.7
19,879 + -2.1
19,879 + -1.1
19,894 + +0.1
19,895 - -0.0
19,913 + +0.7
20,624 - GFF3004 0.81 +0.4
20,624 - GFF3004 0.81 +0.1
20,624 - GFF3004 0.81 +1.0
20,643 - GFF3004 0.86 +0.5
20,702 - +0.1
20,849 + GFF3005 0.12 +0.4
20,938 - GFF3005 0.20 +0.4
20,938 - GFF3005 0.20 +0.3
21,025 - GFF3005 0.28 +0.2
21,087 + GFF3005 0.33 +0.6
21,088 - GFF3005 0.33 +0.0
21,234 - GFF3005 0.47 +0.0
21,306 + GFF3005 0.53 +1.6
21,306 + GFF3005 0.53 +0.4
21,306 + GFF3005 0.53 +1.3
21,307 - GFF3005 0.53 +0.2
21,307 - GFF3005 0.53 +1.0
21,307 - GFF3005 0.53 +0.2
21,336 + GFF3005 0.56 -1.1
21,477 + GFF3005 0.69 -0.9
21,509 + GFF3005 0.72 +0.5
21,512 + GFF3005 0.72 +0.6
21,587 - GFF3005 0.79 +0.7
21,614 + GFF3005 0.81 -0.5
21,619 + GFF3005 0.82 +0.3
21,729 - +0.0
21,844 - -0.1
22,483 - GFF3007 0.18 +1.3
22,650 + GFF3007 0.32 +0.2
22,697 + GFF3007 0.37 +0.5
22,697 + GFF3007 0.37 +0.4
22,724 - GFF3007 0.39 -0.1
22,766 - GFF3007 0.42 +0.2
22,766 - GFF3007 0.42 -0.1

Or see this region's nucleotide sequence