Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2944

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2942 and GFF2943 overlap by 4 nucleotidesGFF2943 and GFF2944 overlap by 32 nucleotidesGFF2944 and GFF2945 are separated by 6 nucleotidesGFF2945 and GFF2946 are separated by 47 nucleotides GFF2942 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (EC 3.1.3.45), at 23,445 to 24,011 GFF2942 GFF2943 - Uncharacterized protein YrbK clustered with lipopolysaccharide transporters, at 24,008 to 24,583 GFF2943 GFF2944 - LptA, protein essential for LPS transport across the periplasm, at 24,552 to 25,106 GFF2944 GFF2945 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 25,113 to 25,838 GFF2945 GFF2946 - RNA polymerase sigma-54 factor RpoN, at 25,886 to 27,319 GFF2946 Position (kb) 24 25 26Strain fitness (log2 ratio) -1 0 1 2 3at 23.571 kb on + strand, within GFF2942at 23.571 kb on + strand, within GFF2942at 23.808 kb on + strand, within GFF2942at 24.436 kb on + strand, within GFF2943at 24.436 kb on + strand, within GFF2943at 24.459 kb on + strand, within GFF2943at 25.863 kb on - strandat 25.941 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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23,571 + GFF2942 0.22 +2.7
23,571 + GFF2942 0.22 +2.9
23,808 + GFF2942 0.64 +3.5
24,436 + GFF2943 0.74 +2.1
24,436 + GFF2943 0.74 -0.4
24,459 + GFF2943 0.78 +1.0
25,863 - +2.7
25,941 + +1.4

Or see this region's nucleotide sequence