Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2942

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2941 and GFF2942 are separated by 20 nucleotidesGFF2942 and GFF2943 overlap by 4 nucleotidesGFF2943 and GFF2944 overlap by 32 nucleotides GFF2941 - Arabinose 5-phosphate isomerase (EC 5.3.1.13), at 22,438 to 23,424 GFF2941 GFF2942 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (EC 3.1.3.45), at 23,445 to 24,011 GFF2942 GFF2943 - Uncharacterized protein YrbK clustered with lipopolysaccharide transporters, at 24,008 to 24,583 GFF2943 GFF2944 - LptA, protein essential for LPS transport across the periplasm, at 24,552 to 25,106 GFF2944 Position (kb) 23 24 25Strain fitness (log2 ratio) -1 0 1 2 3at 22.534 kb on + strandat 22.536 kb on + strandat 22.878 kb on + strand, within GFF2941at 22.926 kb on + strand, within GFF2941at 22.926 kb on + strand, within GFF2941at 22.973 kb on + strand, within GFF2941at 23.042 kb on + strand, within GFF2941at 23.391 kb on + strandat 23.391 kb on + strandat 23.391 kb on + strandat 23.571 kb on + strand, within GFF2942at 23.571 kb on + strand, within GFF2942at 23.808 kb on + strand, within GFF2942at 24.436 kb on + strand, within GFF2943at 24.436 kb on + strand, within GFF2943at 24.459 kb on + strand, within GFF2943

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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22,534 + +2.3
22,536 + +1.7
22,878 + GFF2941 0.45 +2.1
22,926 + GFF2941 0.49 +1.7
22,926 + GFF2941 0.49 +2.3
22,973 + GFF2941 0.54 +1.5
23,042 + GFF2941 0.61 +1.7
23,391 + +1.6
23,391 + +2.6
23,391 + +1.8
23,571 + GFF2942 0.22 +2.7
23,571 + GFF2942 0.22 +2.9
23,808 + GFF2942 0.64 +3.5
24,436 + GFF2943 0.74 +2.1
24,436 + GFF2943 0.74 -0.4
24,459 + GFF2943 0.78 +1.0

Or see this region's nucleotide sequence