Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2675

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2674 and GFF2675 are separated by 96 nucleotidesGFF2675 and GFF2676 are separated by 189 nucleotidesGFF2676 and GFF2677 overlap by 26 nucleotides GFF2674 - FIG00553873: hypothetical protein, at 196,589 to 197,518 GFF2674 GFF2675 - Periplasmic Murein Peptide-Binding Protein MppA, at 197,615 to 199,228 GFF2675 GFF2676 - Gamma-D-Glutamyl-meso-Diaminopimelate Amidase, at 199,418 to 200,146 GFF2676 GFF2677 - L-alanine-DL-glutamate epimerase, at 200,121 to 201,086 GFF2677 Position (kb) 197 198 199 200Strain fitness (log2 ratio) -1 0 1at 196.874 kb on - strand, within GFF2674at 197.086 kb on - strand, within GFF2674at 197.090 kb on - strand, within GFF2674at 197.329 kb on + strand, within GFF2674at 197.341 kb on - strand, within GFF2674at 197.432 kb on - strandat 197.632 kb on - strandat 197.911 kb on - strand, within GFF2675at 198.286 kb on + strand, within GFF2675at 198.941 kb on - strand, within GFF2675at 198.941 kb on - strand, within GFF2675at 198.953 kb on - strand, within GFF2675at 199.085 kb on + strandat 199.377 kb on + strandat 199.476 kb on - strandat 199.504 kb on - strand, within GFF2676at 199.614 kb on + strand, within GFF2676at 199.614 kb on + strand, within GFF2676at 199.645 kb on - strand, within GFF2676at 199.681 kb on - strand, within GFF2676at 199.708 kb on - strand, within GFF2676at 199.848 kb on + strand, within GFF2676at 199.978 kb on - strand, within GFF2676at 199.978 kb on - strand, within GFF2676

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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196,874 - GFF2674 0.31 +0.3
197,086 - GFF2674 0.53 +0.6
197,090 - GFF2674 0.54 -0.6
197,329 + GFF2674 0.80 +0.2
197,341 - GFF2674 0.81 +0.4
197,432 - -0.1
197,632 - +0.0
197,911 - GFF2675 0.18 -0.1
198,286 + GFF2675 0.42 -1.1
198,941 - GFF2675 0.82 -0.4
198,941 - GFF2675 0.82 -1.4
198,953 - GFF2675 0.83 -0.0
199,085 + +1.1
199,377 + -0.4
199,476 - -1.6
199,504 - GFF2676 0.12 -1.1
199,614 + GFF2676 0.27 +0.0
199,614 + GFF2676 0.27 +0.1
199,645 - GFF2676 0.31 +0.3
199,681 - GFF2676 0.36 +0.7
199,708 - GFF2676 0.40 -0.5
199,848 + GFF2676 0.59 -0.6
199,978 - GFF2676 0.77 +0.3
199,978 - GFF2676 0.77 +1.2

Or see this region's nucleotide sequence