Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2668 and GFF2669 are separated by 213 nucleotides GFF2669 and GFF2670 are separated by 203 nucleotides GFF2670 and GFF2671 are separated by 77 nucleotides GFF2671 and GFF2672 are separated by 90 nucleotides
GFF2668 - FIG01046415: hypothetical protein, at 191,262 to 192,371
GFF2668
GFF2669 - Putative outer membrane lipoprotein, at 192,585 to 192,746
GFF2669
GFF2670 - Putative HTH-type transcriptional regulator YjgJ, TetR family, at 192,950 to 193,540
GFF2670
GFF2671 - Uncharacterized oxidoreductase YjgI (EC 1.-.-.-), at 193,618 to 194,331
GFF2671
GFF2672 - oxidoreductase, aldo/keto reductase family, at 194,422 to 195,291
GFF2672
Position (kb) 192
193
194 Strain fitness (log2 ratio) -2
-1
0
1 at 191.961 kb on - strand, within GFF2668 at 191.961 kb on - strand, within GFF2668 at 192.517 kb on + strand at 192.625 kb on - strand, within GFF2669 at 192.653 kb on - strand, within GFF2669 at 192.671 kb on - strand, within GFF2669 at 192.761 kb on + strand at 192.786 kb on + strand at 192.791 kb on + strand at 192.791 kb on + strand at 192.791 kb on + strand at 192.792 kb on - strand at 192.792 kb on - strand at 192.792 kb on - strand at 192.792 kb on - strand at 192.843 kb on + strand at 192.897 kb on - strand at 193.113 kb on + strand, within GFF2670 at 193.114 kb on - strand, within GFF2670 at 193.114 kb on - strand, within GFF2670 at 193.354 kb on - strand, within GFF2670 at 193.681 kb on - strand at 193.685 kb on + strand at 193.686 kb on - strand at 193.932 kb on + strand, within GFF2671 at 193.999 kb on - strand, within GFF2671 at 194.382 kb on - strand at 194.501 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 191,961 - GFF2668 0.63 +1.3 191,961 - GFF2668 0.63 -0.4 192,517 + +1.2 192,625 - GFF2669 0.25 -1.2 192,653 - GFF2669 0.42 -0.5 192,671 - GFF2669 0.53 -0.9 192,761 + -0.6 192,786 + -0.8 192,791 + +0.8 192,791 + +0.2 192,791 + -2.6 192,792 - -0.4 192,792 - -0.0 192,792 - -0.9 192,792 - +1.4 192,843 + +0.5 192,897 - -0.6 193,113 + GFF2670 0.28 -0.8 193,114 - GFF2670 0.28 -0.4 193,114 - GFF2670 0.28 -1.0 193,354 - GFF2670 0.68 -1.1 193,681 - +0.5 193,685 + -0.3 193,686 - -0.3 193,932 + GFF2671 0.44 +0.9 193,999 - GFF2671 0.53 +0.5 194,382 - +0.4 194,501 - -0.3
Or see this region's nucleotide sequence