Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2644 and GFF2645 are separated by 49 nucleotides GFF2645 and GFF2646 overlap by 8 nucleotides GFF2646 and GFF2647 are separated by 18 nucleotides GFF2647 and GFF2648 are separated by 51 nucleotides GFF2648 and GFF2649 are separated by 43 nucleotides
GFF2644 - Universal stress protein F, at 171,749 to 172,183
GFF2644
GFF2645 - Ethidium bromide-methyl viologen resistance protein EmrE, at 172,233 to 172,571
GFF2645
GFF2646 - FIG01046477: hypothetical protein, at 172,564 to 172,716
GFF2646
GFF2647 - Integrase, at 172,735 to 172,875
GFF2647
GFF2648 - tRNA(Cytosine32)-2-thiocytidine synthetase, at 172,927 to 173,862
GFF2648
GFF2649 - ATP-dependent 23S rRNA helicase DbpA, at 173,906 to 175,279
GFF2649
Position (kb) 172
173
174 Strain fitness (log2 ratio) -2
-1
0
1
2 at 172.108 kb on + strand, within GFF2644 at 172.112 kb on - strand, within GFF2644 at 172.116 kb on - strand, within GFF2644 at 172.116 kb on - strand, within GFF2644 at 172.182 kb on - strand at 172.267 kb on - strand, within GFF2645 at 172.345 kb on - strand, within GFF2645 at 172.355 kb on + strand, within GFF2645 at 172.425 kb on - strand, within GFF2645 at 172.425 kb on - strand, within GFF2645 at 172.425 kb on - strand, within GFF2645 at 172.425 kb on - strand, within GFF2645 at 172.457 kb on + strand, within GFF2645 at 172.542 kb on - strand at 172.565 kb on + strand at 172.571 kb on + strand at 172.571 kb on + strand at 172.615 kb on - strand, within GFF2646 at 172.619 kb on - strand, within GFF2646 at 172.659 kb on + strand, within GFF2646 at 172.702 kb on + strand at 172.716 kb on + strand at 172.717 kb on - strand at 172.717 kb on - strand at 172.717 kb on - strand at 173.079 kb on + strand, within GFF2648 at 173.080 kb on - strand, within GFF2648 at 173.080 kb on - strand, within GFF2648 at 173.080 kb on - strand, within GFF2648 at 173.083 kb on + strand, within GFF2648 at 173.084 kb on - strand, within GFF2648 at 173.085 kb on + strand, within GFF2648 at 173.187 kb on + strand, within GFF2648 at 173.188 kb on - strand, within GFF2648 at 173.200 kb on + strand, within GFF2648 at 173.512 kb on - strand, within GFF2648 at 173.656 kb on - strand, within GFF2648 at 174.038 kb on - strand at 174.334 kb on - strand, within GFF2649 at 174.358 kb on + strand, within GFF2649 at 174.497 kb on - strand, within GFF2649 at 174.572 kb on - strand, within GFF2649 at 174.651 kb on - strand, within GFF2649 at 174.671 kb on + strand, within GFF2649 at 174.671 kb on + strand, within GFF2649 at 174.671 kb on + strand, within GFF2649 at 174.686 kb on + strand, within GFF2649
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 172,108 + GFF2644 0.83 -0.6 172,112 - GFF2644 0.83 -0.5 172,116 - GFF2644 0.84 +0.1 172,116 - GFF2644 0.84 -0.1 172,182 - -0.2 172,267 - GFF2645 0.10 -0.5 172,345 - GFF2645 0.33 -0.5 172,355 + GFF2645 0.36 -0.9 172,425 - GFF2645 0.57 +0.2 172,425 - GFF2645 0.57 +1.2 172,425 - GFF2645 0.57 -0.0 172,425 - GFF2645 0.57 -1.7 172,457 + GFF2645 0.66 +0.0 172,542 - +1.0 172,565 + -0.3 172,571 + +0.3 172,571 + -1.3 172,615 - GFF2646 0.33 +1.0 172,619 - GFF2646 0.36 -1.3 172,659 + GFF2646 0.62 -0.5 172,702 + -0.7 172,716 + -0.9 172,717 - +0.8 172,717 - +1.6 172,717 - +0.2 173,079 + GFF2648 0.16 +1.1 173,080 - GFF2648 0.16 +1.2 173,080 - GFF2648 0.16 +1.1 173,080 - GFF2648 0.16 +1.3 173,083 + GFF2648 0.17 +1.0 173,084 - GFF2648 0.17 +1.0 173,085 + GFF2648 0.17 +0.9 173,187 + GFF2648 0.28 -0.9 173,188 - GFF2648 0.28 +0.7 173,200 + GFF2648 0.29 +1.1 173,512 - GFF2648 0.62 +0.4 173,656 - GFF2648 0.78 +1.3 174,038 - -0.6 174,334 - GFF2649 0.31 +0.1 174,358 + GFF2649 0.33 +0.4 174,497 - GFF2649 0.43 +2.0 174,572 - GFF2649 0.48 +0.5 174,651 - GFF2649 0.54 +0.9 174,671 + GFF2649 0.56 -1.9 174,671 + GFF2649 0.56 -1.2 174,671 + GFF2649 0.56 +1.1 174,686 + GFF2649 0.57 +0.2
Or see this region's nucleotide sequence