Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2558

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2557 and GFF2558 are separated by 90 nucleotidesGFF2558 and GFF2559 overlap by 8 nucleotidesGFF2559 and GFF2560 are separated by 13 nucleotides GFF2557 - Alcohol dehydrogenase (EC 1.1.1.1), at 73,411 to 74,421 GFF2557 GFF2558 - Formate dehydrogenase N gamma subunit (EC 1.2.1.2), at 74,512 to 75,168 GFF2558 GFF2559 - Formate dehydrogenase N beta subunit (EC 1.2.1.2), at 75,161 to 76,045 GFF2559 GFF2560 - Formate dehydrogenase N alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 76,059 to 79,106 GFF2560 Position (kb) 74 75 76Strain fitness (log2 ratio) -3 -2 -1 0 1at 73.532 kb on + strand, within GFF2557at 73.666 kb on + strand, within GFF2557at 73.666 kb on + strand, within GFF2557at 73.774 kb on - strand, within GFF2557at 73.887 kb on + strand, within GFF2557at 73.887 kb on + strand, within GFF2557at 73.888 kb on - strand, within GFF2557at 73.912 kb on + strand, within GFF2557at 74.043 kb on + strand, within GFF2557at 74.148 kb on - strand, within GFF2557at 74.398 kb on - strandat 74.445 kb on + strandat 74.658 kb on - strand, within GFF2558at 74.658 kb on - strand, within GFF2558at 74.658 kb on - strand, within GFF2558at 74.661 kb on + strand, within GFF2558at 74.662 kb on - strand, within GFF2558at 74.662 kb on - strand, within GFF2558at 74.662 kb on - strand, within GFF2558at 74.718 kb on + strand, within GFF2558at 74.719 kb on - strand, within GFF2558at 74.808 kb on + strand, within GFF2558at 74.809 kb on - strand, within GFF2558at 74.844 kb on - strand, within GFF2558at 74.844 kb on - strand, within GFF2558at 74.865 kb on + strand, within GFF2558at 74.866 kb on - strand, within GFF2558at 74.885 kb on - strand, within GFF2558at 75.050 kb on - strand, within GFF2558at 75.220 kb on - strandat 75.220 kb on - strandat 75.369 kb on + strand, within GFF2559at 75.409 kb on + strand, within GFF2559at 75.745 kb on + strand, within GFF2559at 75.745 kb on + strand, within GFF2559at 75.746 kb on - strand, within GFF2559at 75.850 kb on - strand, within GFF2559at 75.883 kb on + strand, within GFF2559at 75.969 kb on + strandat 76.045 kb on + strandat 76.046 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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73,532 + GFF2557 0.12 +0.1
73,666 + GFF2557 0.25 +0.2
73,666 + GFF2557 0.25 -0.0
73,774 - GFF2557 0.36 +0.9
73,887 + GFF2557 0.47 +1.5
73,887 + GFF2557 0.47 -2.2
73,888 - GFF2557 0.47 +1.5
73,912 + GFF2557 0.50 -0.3
74,043 + GFF2557 0.63 -0.5
74,148 - GFF2557 0.73 +1.3
74,398 - +0.2
74,445 + -1.3
74,658 - GFF2558 0.22 -0.5
74,658 - GFF2558 0.22 -0.3
74,658 - GFF2558 0.22 -1.1
74,661 + GFF2558 0.23 -2.8
74,662 - GFF2558 0.23 -0.3
74,662 - GFF2558 0.23 -0.1
74,662 - GFF2558 0.23 -0.3
74,718 + GFF2558 0.31 +0.5
74,719 - GFF2558 0.32 -0.2
74,808 + GFF2558 0.45 +0.3
74,809 - GFF2558 0.45 -0.4
74,844 - GFF2558 0.51 +0.7
74,844 - GFF2558 0.51 -0.4
74,865 + GFF2558 0.54 +0.9
74,866 - GFF2558 0.54 -0.8
74,885 - GFF2558 0.57 +1.0
75,050 - GFF2558 0.82 -0.4
75,220 - -0.6
75,220 - +0.0
75,369 + GFF2559 0.24 +0.4
75,409 + GFF2559 0.28 +0.0
75,745 + GFF2559 0.66 +0.2
75,745 + GFF2559 0.66 +0.4
75,746 - GFF2559 0.66 +0.6
75,850 - GFF2559 0.78 -0.2
75,883 + GFF2559 0.82 +0.3
75,969 + -1.0
76,045 + +0.0
76,046 - -0.3

Or see this region's nucleotide sequence