Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2304 and GFF2305 are separated by 106 nucleotides GFF2305 and GFF2306 are separated by 96 nucleotides GFF2306 and GFF2307 are separated by 111 nucleotides
GFF2304 - Putative membrane protein, at 90,766 to 91,194
GFF2304
GFF2305 - Ferredoxin--NADP(+) reductase (EC 1.18.1.2), at 91,301 to 92,047
GFF2305
GFF2306 - Fructose-1,6-bisphosphatase, GlpX type (EC 3.1.3.11), at 92,144 to 93,154
GFF2306
GFF2307 - Glycerol kinase (EC 2.7.1.30), at 93,266 to 94,774
GFF2307
Position (kb) 92
93
94 Strain fitness (log2 ratio) -1
0
1
2 at 91.325 kb on - strand at 91.563 kb on + strand, within GFF2305 at 91.673 kb on + strand, within GFF2305 at 91.674 kb on - strand, within GFF2305 at 91.674 kb on - strand, within GFF2305 at 91.674 kb on - strand, within GFF2305 at 91.674 kb on - strand, within GFF2305 at 91.852 kb on - strand, within GFF2305 at 92.018 kb on - strand at 92.077 kb on + strand at 92.078 kb on - strand at 92.078 kb on - strand at 92.133 kb on + strand at 92.153 kb on - strand at 92.176 kb on + strand at 92.187 kb on + strand at 92.194 kb on + strand at 92.320 kb on + strand, within GFF2306 at 92.320 kb on + strand, within GFF2306 at 92.567 kb on - strand, within GFF2306 at 92.567 kb on - strand, within GFF2306 at 92.798 kb on - strand, within GFF2306 at 92.799 kb on + strand, within GFF2306 at 92.799 kb on + strand, within GFF2306 at 92.799 kb on + strand, within GFF2306 at 92.800 kb on - strand, within GFF2306 at 92.800 kb on - strand, within GFF2306 at 92.800 kb on - strand, within GFF2306 at 92.831 kb on - strand, within GFF2306 at 92.955 kb on + strand, within GFF2306 at 92.955 kb on + strand, within GFF2306 at 93.031 kb on + strand, within GFF2306 at 93.031 kb on + strand, within GFF2306 at 93.032 kb on - strand, within GFF2306 at 93.190 kb on + strand at 93.191 kb on - strand at 93.191 kb on - strand at 93.267 kb on + strand at 93.267 kb on + strand at 93.267 kb on + strand at 93.268 kb on - strand at 93.330 kb on + strand at 93.331 kb on - strand at 93.335 kb on - strand at 93.757 kb on + strand, within GFF2307 at 93.757 kb on + strand, within GFF2307 at 93.758 kb on - strand, within GFF2307 at 93.873 kb on + strand, within GFF2307 at 93.978 kb on + strand, within GFF2307 at 94.063 kb on + strand, within GFF2307 at 94.063 kb on + strand, within GFF2307 at 94.063 kb on + strand, within GFF2307 at 94.063 kb on + strand, within GFF2307 at 94.063 kb on + strand, within GFF2307 at 94.064 kb on - strand, within GFF2307 at 94.064 kb on - strand, within GFF2307 at 94.069 kb on + strand, within GFF2307 at 94.078 kb on + strand, within GFF2307 at 94.117 kb on + strand, within GFF2307 at 94.117 kb on + strand, within GFF2307 at 94.117 kb on + strand, within GFF2307 at 94.118 kb on - strand, within GFF2307 at 94.118 kb on - strand, within GFF2307 at 94.121 kb on + strand, within GFF2307 at 94.122 kb on - strand, within GFF2307
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 91,325 - -0.4 91,563 + GFF2305 0.35 -0.3 91,673 + GFF2305 0.50 -0.9 91,674 - GFF2305 0.50 +0.8 91,674 - GFF2305 0.50 +1.6 91,674 - GFF2305 0.50 +0.3 91,674 - GFF2305 0.50 -0.5 91,852 - GFF2305 0.74 +0.3 92,018 - -0.0 92,077 + +0.3 92,078 - -0.2 92,078 - +0.0 92,133 + +0.4 92,153 - -0.4 92,176 + +0.8 92,187 + +1.1 92,194 + -0.0 92,320 + GFF2306 0.17 +0.7 92,320 + GFF2306 0.17 +0.6 92,567 - GFF2306 0.42 +0.1 92,567 - GFF2306 0.42 +0.9 92,798 - GFF2306 0.65 -0.3 92,799 + GFF2306 0.65 +0.3 92,799 + GFF2306 0.65 +0.5 92,799 + GFF2306 0.65 -0.5 92,800 - GFF2306 0.65 -0.3 92,800 - GFF2306 0.65 +1.0 92,800 - GFF2306 0.65 +0.1 92,831 - GFF2306 0.68 +0.3 92,955 + GFF2306 0.80 +1.5 92,955 + GFF2306 0.80 -0.1 93,031 + GFF2306 0.88 +0.7 93,031 + GFF2306 0.88 +0.4 93,032 - GFF2306 0.88 +1.0 93,190 + -0.4 93,191 - +0.6 93,191 - -0.7 93,267 + +0.8 93,267 + +0.2 93,267 + -0.2 93,268 - +0.1 93,330 + +0.8 93,331 - +0.5 93,335 - +2.1 93,757 + GFF2307 0.33 +0.8 93,757 + GFF2307 0.33 +1.0 93,758 - GFF2307 0.33 +0.6 93,873 + GFF2307 0.40 +0.6 93,978 + GFF2307 0.47 +1.0 94,063 + GFF2307 0.53 +0.8 94,063 + GFF2307 0.53 -1.0 94,063 + GFF2307 0.53 +1.0 94,063 + GFF2307 0.53 +0.6 94,063 + GFF2307 0.53 +2.1 94,064 - GFF2307 0.53 +1.1 94,064 - GFF2307 0.53 +1.1 94,069 + GFF2307 0.53 +1.5 94,078 + GFF2307 0.54 +0.2 94,117 + GFF2307 0.56 +0.8 94,117 + GFF2307 0.56 +0.6 94,117 + GFF2307 0.56 +0.6 94,118 - GFF2307 0.56 +0.7 94,118 - GFF2307 0.56 +1.0 94,121 + GFF2307 0.57 +0.1 94,122 - GFF2307 0.57 +1.4
Or see this region's nucleotide sequence