Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2191 and GFF2192 are separated by 203 nucleotides GFF2192 and GFF2193 are separated by 124 nucleotides GFF2193 and GFF2194 are separated by 127 nucleotides GFF2194 and GFF2195 are separated by 62 nucleotides
GFF2191 - Beta-lactamase class C and other penicillin binding proteins, at 42,157 to 43,455
GFF2191
GFF2192 - GDP-mannose pyrophosphatase YffH, at 43,659 to 44,234
GFF2192
GFF2193 - FIG01200701: possible membrane protein, at 44,359 to 45,402
GFF2193
GFF2194 - FIG01046050: hypothetical protein, at 45,530 to 45,760
GFF2194
GFF2195 - Transketolase (EC 2.2.1.1), at 45,823 to 47,823
GFF2195
Position (kb) 44
45
46 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 43.426 kb on + strand at 43.531 kb on - strand at 43.531 kb on - strand at 43.556 kb on - strand at 43.564 kb on - strand at 43.630 kb on + strand at 43.727 kb on + strand, within GFF2192 at 43.816 kb on - strand, within GFF2192 at 43.898 kb on - strand, within GFF2192 at 43.946 kb on + strand, within GFF2192 at 44.002 kb on + strand, within GFF2192 at 44.003 kb on - strand, within GFF2192 at 44.013 kb on + strand, within GFF2192 at 44.013 kb on + strand, within GFF2192 at 44.014 kb on - strand, within GFF2192 at 44.015 kb on + strand, within GFF2192 at 44.015 kb on + strand, within GFF2192 at 44.016 kb on - strand, within GFF2192 at 44.016 kb on - strand, within GFF2192 at 44.065 kb on - strand, within GFF2192 at 44.072 kb on + strand, within GFF2192 at 44.195 kb on - strand at 44.526 kb on - strand, within GFF2193 at 44.624 kb on + strand, within GFF2193 at 44.804 kb on + strand, within GFF2193 at 44.812 kb on - strand, within GFF2193 at 45.058 kb on - strand, within GFF2193 at 45.094 kb on + strand, within GFF2193 at 45.097 kb on - strand, within GFF2193 at 45.156 kb on - strand, within GFF2193 at 45.174 kb on + strand, within GFF2193 at 45.354 kb on + strand at 45.382 kb on - strand at 45.464 kb on + strand at 45.474 kb on + strand at 45.613 kb on - strand, within GFF2194 at 45.838 kb on + strand at 45.962 kb on + strand at 45.962 kb on + strand at 45.983 kb on - strand at 46.138 kb on - strand, within GFF2195 at 46.174 kb on - strand, within GFF2195
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 43,426 + -0.9 43,531 - -0.5 43,531 - -1.4 43,556 - -1.5 43,564 - +0.3 43,630 + -0.3 43,727 + GFF2192 0.12 -0.9 43,816 - GFF2192 0.27 -0.1 43,898 - GFF2192 0.41 +0.1 43,946 + GFF2192 0.50 -0.3 44,002 + GFF2192 0.60 -0.3 44,003 - GFF2192 0.60 -2.6 44,013 + GFF2192 0.61 -0.2 44,013 + GFF2192 0.61 +0.1 44,014 - GFF2192 0.62 -3.1 44,015 + GFF2192 0.62 +0.7 44,015 + GFF2192 0.62 -0.1 44,016 - GFF2192 0.62 +0.2 44,016 - GFF2192 0.62 +1.1 44,065 - GFF2192 0.70 -1.6 44,072 + GFF2192 0.72 +0.4 44,195 - +1.8 44,526 - GFF2193 0.16 +0.8 44,624 + GFF2193 0.25 +1.7 44,804 + GFF2193 0.43 +0.9 44,812 - GFF2193 0.43 +1.4 45,058 - GFF2193 0.67 +1.3 45,094 + GFF2193 0.70 -0.1 45,097 - GFF2193 0.71 +1.1 45,156 - GFF2193 0.76 +0.7 45,174 + GFF2193 0.78 +1.1 45,354 + +1.3 45,382 - +1.3 45,464 + -1.1 45,474 + +0.9 45,613 - GFF2194 0.36 +0.0 45,838 + -0.8 45,962 + +0.4 45,962 + +0.0 45,983 - -0.8 46,138 - GFF2195 0.16 -0.5 46,174 - GFF2195 0.18 -0.5
Or see this region's nucleotide sequence