Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1933

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1932 and GFF1933 are separated by 149 nucleotidesGFF1933 and GFF1934 are separated by 48 nucleotidesGFF1934 and GFF1935 overlap by 1 nucleotides GFF1932 - Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2), at 125,080 to 125,994 GFF1932 GFF1933 - DedA protein, at 126,144 to 126,803 GFF1933 GFF1934 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 126,852 to 127,664 GFF1934 GFF1935 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 127,664 to 128,677 GFF1935 Position (kb) 126 127Strain fitness (log2 ratio) -1 0 1 2 3at 126.032 kb on - strandat 126.138 kb on + strandat 126.240 kb on - strand, within GFF1933at 126.327 kb on - strand, within GFF1933at 126.327 kb on - strand, within GFF1933at 126.327 kb on - strand, within GFF1933at 126.328 kb on + strand, within GFF1933at 126.786 kb on - strandat 126.853 kb on + strandat 127.047 kb on - strand, within GFF1934at 127.086 kb on - strand, within GFF1934at 127.099 kb on + strand, within GFF1934at 127.247 kb on - strand, within GFF1934at 127.273 kb on - strand, within GFF1934at 127.298 kb on - strand, within GFF1934at 127.305 kb on - strand, within GFF1934at 127.357 kb on - strand, within GFF1934at 127.514 kb on + strand, within GFF1934at 127.515 kb on - strand, within GFF1934at 127.598 kb on - strandat 127.675 kb on - strandat 127.675 kb on - strandat 127.799 kb on - strand, within GFF1935

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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126,032 - +0.8
126,138 + +1.2
126,240 - GFF1933 0.15 +0.1
126,327 - GFF1933 0.28 -0.8
126,327 - GFF1933 0.28 -1.7
126,327 - GFF1933 0.28 -0.2
126,328 + GFF1933 0.28 -0.6
126,786 - -1.0
126,853 + -0.7
127,047 - GFF1934 0.24 +3.6
127,086 - GFF1934 0.29 +1.1
127,099 + GFF1934 0.30 +2.9
127,247 - GFF1934 0.49 +1.8
127,273 - GFF1934 0.52 +1.0
127,298 - GFF1934 0.55 +2.3
127,305 - GFF1934 0.56 +1.4
127,357 - GFF1934 0.62 +0.5
127,514 + GFF1934 0.81 +1.2
127,515 - GFF1934 0.82 +2.5
127,598 - +2.1
127,675 - +0.7
127,675 - +2.6
127,799 - GFF1935 0.13 -1.6

Or see this region's nucleotide sequence