Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1711 and GFF1712 overlap by 7 nucleotides GFF1712 and GFF1713 are separated by 4 nucleotides
GFF1711 - Putative regulator protein, at 10,593 to 12,089
GFF1711
GFF1712 - FIG00637882: hypothetical protein, at 12,083 to 13,534
GFF1712
GFF1713 - Aspartate--ammonia ligase (EC 6.3.1.1), at 13,539 to 14,531
GFF1713
Position (kb) 12
13
14 Strain fitness (log2 ratio) -1
0
1
2 at 11.226 kb on + strand, within GFF1711 at 11.291 kb on + strand, within GFF1711 at 11.292 kb on - strand, within GFF1711 at 11.313 kb on - strand, within GFF1711 at 11.332 kb on - strand, within GFF1711 at 11.424 kb on - strand, within GFF1711 at 11.588 kb on + strand, within GFF1711 at 11.621 kb on + strand, within GFF1711 at 11.622 kb on - strand, within GFF1711 at 11.643 kb on + strand, within GFF1711 at 11.644 kb on - strand, within GFF1711 at 11.684 kb on - strand, within GFF1711 at 11.715 kb on - strand, within GFF1711 at 11.732 kb on + strand, within GFF1711 at 11.873 kb on - strand, within GFF1711 at 11.880 kb on - strand, within GFF1711 at 11.915 kb on + strand, within GFF1711 at 11.976 kb on - strand at 12.055 kb on + strand at 12.056 kb on - strand at 12.056 kb on - strand at 12.106 kb on + strand at 12.106 kb on + strand at 12.107 kb on - strand at 12.311 kb on + strand, within GFF1712 at 12.399 kb on - strand, within GFF1712 at 12.436 kb on + strand, within GFF1712 at 12.511 kb on - strand, within GFF1712 at 12.522 kb on + strand, within GFF1712 at 12.523 kb on - strand, within GFF1712 at 12.613 kb on + strand, within GFF1712 at 12.638 kb on - strand, within GFF1712 at 12.655 kb on + strand, within GFF1712 at 12.669 kb on - strand, within GFF1712 at 12.718 kb on - strand, within GFF1712 at 12.862 kb on + strand, within GFF1712 at 12.863 kb on - strand, within GFF1712 at 13.049 kb on - strand, within GFF1712 at 13.049 kb on - strand, within GFF1712 at 13.063 kb on + strand, within GFF1712 at 13.087 kb on + strand, within GFF1712 at 13.087 kb on + strand, within GFF1712 at 13.159 kb on + strand, within GFF1712 at 13.163 kb on - strand, within GFF1712 at 13.478 kb on - strand at 13.604 kb on + strand at 13.605 kb on - strand at 13.879 kb on + strand, within GFF1713 at 13.879 kb on + strand, within GFF1713 at 13.880 kb on - strand, within GFF1713 at 14.076 kb on - strand, within GFF1713 at 14.099 kb on + strand, within GFF1713 at 14.114 kb on + strand, within GFF1713 at 14.195 kb on - strand, within GFF1713 at 14.254 kb on + strand, within GFF1713 at 14.393 kb on + strand, within GFF1713 at 14.393 kb on + strand, within GFF1713 at 14.403 kb on - strand, within GFF1713
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 11,226 + GFF1711 0.42 -0.1 11,291 + GFF1711 0.47 +0.1 11,292 - GFF1711 0.47 -0.4 11,313 - GFF1711 0.48 +0.9 11,332 - GFF1711 0.49 +0.2 11,424 - GFF1711 0.56 -0.8 11,588 + GFF1711 0.66 +0.2 11,621 + GFF1711 0.69 +1.9 11,622 - GFF1711 0.69 +2.2 11,643 + GFF1711 0.70 +0.8 11,644 - GFF1711 0.70 -1.0 11,684 - GFF1711 0.73 +0.5 11,715 - GFF1711 0.75 +0.4 11,732 + GFF1711 0.76 -0.6 11,873 - GFF1711 0.86 +0.2 11,880 - GFF1711 0.86 -0.1 11,915 + GFF1711 0.88 +0.2 11,976 - +1.4 12,055 + +0.1 12,056 - +0.1 12,056 - -0.2 12,106 + +0.5 12,106 + -0.3 12,107 - +0.2 12,311 + GFF1712 0.16 -1.0 12,399 - GFF1712 0.22 -0.8 12,436 + GFF1712 0.24 +0.2 12,511 - GFF1712 0.29 +0.1 12,522 + GFF1712 0.30 +0.1 12,523 - GFF1712 0.30 -0.3 12,613 + GFF1712 0.37 -0.1 12,638 - GFF1712 0.38 -0.1 12,655 + GFF1712 0.39 +0.2 12,669 - GFF1712 0.40 +0.3 12,718 - GFF1712 0.44 -0.1 12,862 + GFF1712 0.54 +0.7 12,863 - GFF1712 0.54 +0.7 13,049 - GFF1712 0.67 -0.2 13,049 - GFF1712 0.67 +0.0 13,063 + GFF1712 0.67 +0.0 13,087 + GFF1712 0.69 +0.1 13,087 + GFF1712 0.69 +0.6 13,159 + GFF1712 0.74 -0.1 13,163 - GFF1712 0.74 -0.3 13,478 - +1.5 13,604 + +1.0 13,605 - +0.6 13,879 + GFF1713 0.34 +0.6 13,879 + GFF1713 0.34 +0.2 13,880 - GFF1713 0.34 +0.3 14,076 - GFF1713 0.54 +0.5 14,099 + GFF1713 0.56 -0.2 14,114 + GFF1713 0.58 -0.7 14,195 - GFF1713 0.66 +0.1 14,254 + GFF1713 0.72 -0.4 14,393 + GFF1713 0.86 -0.7 14,393 + GFF1713 0.86 +0.1 14,403 - GFF1713 0.87 +0.0
Or see this region's nucleotide sequence